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Literature summary extracted from

  • Kotani, T.; Takagi, H.
    Identification of amino acid residues essential for the yeast N-acetyltransferase Mpr1 activity by site-directed mutagenesis (2008), FEMS Yeast Res., 8, 607-614 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.271 expressed in Saccharomyces cerevisiae strain CKY263 Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.271 R145A the mutation causes a large reduction in the affinity for l-azetidine-2-carboxylic acid and acetyl-CoA Saccharomyces cerevisiae
2.3.1.271 R145D the mutation causes a large reduction in the affinity for l-azetidine-2-carboxylic acid and acetyl-CoA Saccharomyces cerevisiae
2.3.1.271 R145E the mutation causes a large reduction in the affinity for l-azetidine-2-carboxylic acid and acetyl-CoA Saccharomyces cerevisiae
2.3.1.271 R145G the mutation causes a large reduction in the affinity for l-azetidine-2-carboxylic acid and acetyl-CoA Saccharomyces cerevisiae
2.3.1.271 R145W the mutation causes a large reduction in the affinity for l-azetidine-2-carboxylic acid and acetyl-CoA Saccharomyces cerevisiae
2.3.1.271 Y121A the mutation causes a large reduction in the affinity for l-azetidine-2-carboxylic acid and increases affinity for acetyl-CoA Saccharomyces cerevisiae
2.3.1.271 Y157A the mutation causes a large reduction in the affinity for l-azetidine-2-carboxylic acid and increases affinity for acetyl-CoA Saccharomyces cerevisiae
2.3.1.271 Y166A inactive Saccharomyces cerevisiae
2.3.1.271 Y166F the mutation causes a large reduction in the affinity for l-azetidine-2-carboxylic acid and increases affinity for acetyl-CoA Saccharomyces cerevisiae
2.3.1.271 Y166F the mutation leads to a remarkable decrease of the kcat/Km value for l-azetidine-2-carboxylic acid compared to the wild type enzyme Saccharomyces cerevisiae
2.3.1.271 Y168A the mutation causes a large reduction in the affinity for l-azetidine-2-carboxylic acid and increases affinity for acetyl-CoA Saccharomyces cerevisiae
2.3.1.271 Y75A the mutation causes a large reduction in the affinity for l-azetidine-2-carboxylic acid and acetyl-CoA Saccharomyces cerevisiae
2.3.1.271 Y89A the mutation causes a large reduction in the affinity for l-azetidine-2-carboxylic acid and increases affinity for acetyl-CoA Saccharomyces cerevisiae
2.3.1.271 Y989A the mutation results in a 2.6fold reduction in the kcat/Km ratio for l-azetidine-2-carboxylic acid compared to the wild type enzyme Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.271 0.00689
-
 acetyl-CoA mutant enzyme Y168A, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 0.00761
-
 acetyl-CoA mutant enzyme Y157A, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 0.0087
-
 acetyl-CoA mutant enzyme Y121A, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 0.00928
-
 acetyl-CoA mutant enzyme Y166F, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 0.0179
-
 acetyl-CoA mutant enzyme Y89A, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 0.0282
-
 acetyl-CoA wild type enzyme, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 0.0456
-
 acetyl-CoA mutant enzyme Y75A, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 0.569
-
 acetyl-CoA mutant enzyme R145A, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 0.747
-
 acetyl-CoA mutant enzyme R145W, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 1.076
-
 acetyl-CoA mutant enzyme R145G, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 1.17
-
 L-azetidine-2-carboxylic acid mutant enzyme Y168A, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 1.5 2 L-azetidine-2-carboxylic acid wild type enzyme, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 2.91
-
 L-azetidine-2-carboxylic acid mutant enzyme Y89A, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 3.144
-
 acetyl-CoA mutant enzyme R145E, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 3.387
-
 acetyl-CoA mutant enzyme R145D, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 3.49
-
 L-azetidine-2-carboxylic acid mutant enzyme Y157A, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 4.03
-
 L-azetidine-2-carboxylic acid mutant enzyme Y166F, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 12.7
-
 L-azetidine-2-carboxylic acid mutant enzyme Y121A, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 60.9
-
 L-azetidine-2-carboxylic acid mutant enzyme R145A, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 64.4
-
 L-azetidine-2-carboxylic acid mutant enzyme R145G, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 78.4
-
 L-azetidine-2-carboxylic acid mutant enzyme R145W, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 92.7
-
 L-azetidine-2-carboxylic acid mutant enzyme Y75A, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 116
-
 L-azetidine-2-carboxylic acid mutant enzyme R145E, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 192
-
 L-azetidine-2-carboxylic acid mutant enzyme R145D, at pH 8.5 and 30°C Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.271 Saccharomyces cerevisiae E9P8D2
-
-
2.3.1.271 Saccharomyces cerevisiae Sigma1278b E9P8D2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.271 acetyl-CoA + L-azetidine-2-carboxylic acid
-
 Saccharomyces cerevisiae CoA + N-acetyl-L-azetidine-2-carboxylic acid
-
 ?
2.3.1.271 acetyl-CoA + L-azetidine-2-carboxylic acid
-
 Saccharomyces cerevisiae Sigma1278b CoA + N-acetyl-L-azetidine-2-carboxylic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.271 ? x * 27900, SDS-PAGE Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
2.3.1.271 MPR1
-
 Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.271 50
-
 the enzyme retains 90% activity after 5 min at 50°C Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.271 4.31
-
 L-azetidine-2-carboxylic acid mutant enzyme Y157A, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 7.09
-
 L-azetidine-2-carboxylic acid mutant enzyme R145D, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 12.8
-
 L-azetidine-2-carboxylic acid mutant enzyme Y121A, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 13.7
-
 L-azetidine-2-carboxylic acid mutant enzyme Y166F, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 19.3
-
 L-azetidine-2-carboxylic acid mutant enzyme Y168A, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 24.8
-
 L-azetidine-2-carboxylic acid mutant enzyme Y89A, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 33.5
-
 L-azetidine-2-carboxylic acid wild type enzyme, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 63.1
-
 L-azetidine-2-carboxylic acid mutant enzyme R145E, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 73.6
-
 L-azetidine-2-carboxylic acid mutant enzyme Y75A, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 225
-
 L-azetidine-2-carboxylic acid mutant enzyme R145W, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 304
-
 L-azetidine-2-carboxylic acid mutant enzyme R145G, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 609
-
 L-azetidine-2-carboxylic acid mutant enzyme R145A, at pH 8.5 and 30°C Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
2.3.1.271 metabolism the enzyme confers resistance to the proline analogue azetidine-2-carboxylate Saccharomyces cerevisiae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.271 0.0369
-
 L-azetidine-2-carboxylic acid mutant enzyme R145D, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 0.548
-
 L-azetidine-2-carboxylic acid mutant enzyme R145E, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 0.794
-
 L-azetidine-2-carboxylic acid mutant enzyme Y75A, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 1
-
 L-azetidine-2-carboxylic acid mutant enzyme Y121A, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 1.24
-
 L-azetidine-2-carboxylic acid mutant enzyme Y157A, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 2.12
-
 acetyl-CoA mutant enzyme R145D, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 2.88
-
 L-azetidine-2-carboxylic acid mutant enzyme R145W, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 3.41
-
 L-azetidine-2-carboxylic acid mutant enzyme Y166F, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 4.72
-
 L-azetidine-2-carboxylic acid mutant enzyme R145G, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 8.51
-
 L-azetidine-2-carboxylic acid mutant enzyme Y89A, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 10
-
 L-azetidine-2-carboxylic acid mutant enzyme R145A, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 16.5
-
 L-azetidine-2-carboxylic acid mutant enzyme Y168A, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 20.1
-
 acetyl-CoA mutant enzyme R145E, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 22.4
-
 L-azetidine-2-carboxylic acid wild type enzyme, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 283
-
 acetyl-CoA mutant enzyme R145G, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 302
-
 acetyl-CoA mutant enzyme R145W, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 569
-
 acetyl-CoA mutant enzyme Y157A, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 1071
-
 acetyl-CoA mutant enzyme R145A, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 1190
-
 acetyl-CoA wild type enzyme, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 1392
-
 acetyl-CoA mutant enzyme Y89A, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 1470
-
 acetyl-CoA mutant enzyme Y121A, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 1481
-
 acetyl-CoA mutant enzyme Y166F, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 1615
-
 acetyl-CoA mutant enzyme Y75A, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 2814
-
 acetyl-CoA mutant enzyme Y168A, at pH 8.5 and 30°C Saccharomyces cerevisiae