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Literature summary extracted from

  • Heath, C.; Jeffries, A.; Hough, D.; Danson, M.
    Discovery of the catalytic function of a putative 2-oxoacid dehydrogenase multienzyme complex in the thermophilic archaeon Thermoplasma acidophilum (2004), FEBS Lett., 577, 523-527 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.72 expression in Escherichia coli Thermoplasma acidophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.72 0.027
-
3-methyl-2-oxobutanoate pH 7.0, 55°C Thermoplasma acidophilum
4.1.1.72 0.042
-
4-methyl-2-oxopentanoate pH 7.0, 55°C Thermoplasma acidophilum
4.1.1.72 0.1
-
3-methyl-2-oxopentanoate pH 7.0, 55°C Thermoplasma acidophilum
4.1.1.72 0.6
-
pyruvate pH 7.0, 55°C Thermoplasma acidophilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.72 165000
-
gel filtration Thermoplasma acidophilum

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.72 Thermoplasma acidophilum Q9HIA4 AND Q9HIA3 Q9HIA4: chain E1-beta, Q9HIA3: chain E1-alpha
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.72
-
Thermoplasma acidophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.72 3-methyl-2-oxobutanoate
-
Thermoplasma acidophilum 2-methylpropanal + CO2
-
?
4.1.1.72 3-methyl-2-oxopentanoate
-
Thermoplasma acidophilum 2-methylbutanal + CO2
-
?
4.1.1.72 4-methyl-2-oxopentanoate
-
Thermoplasma acidophilum 4-methylbutanal + CO2
-
?
4.1.1.72 additional information no activity with 2-oxoglutarate Thermoplasma acidophilum ?
-
?
4.1.1.72 pyruvate
-
Thermoplasma acidophilum acetaldehyde + CO2
-
?

Subunits

EC Number Subunits Comment Organism
4.1.1.72 tetramer 2 * 43000 (alpha) + 2 * 37000 (beta), SDS-PAGE Thermoplasma acidophilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.72 55
-
assay at Thermoplasma acidophilum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.72 0.09
-
pyruvate pH 7.0, 55°C Thermoplasma acidophilum
4.1.1.72 0.17
-
4-methyl-2-oxopentanoate pH 7.0, 55°C Thermoplasma acidophilum
4.1.1.72 0.3
-
3-methyl-2-oxobutanoate pH 7.0, 55°C Thermoplasma acidophilum
4.1.1.72 0.5
-
3-methyl-2-oxopentanoate pH 7.0, 55°C Thermoplasma acidophilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.72 7
-
assay at Thermoplasma acidophilum

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.72 thiamine diphosphate required Thermoplasma acidophilum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.1.72 0.15
-
pyruvate pH 7.0, 55°C Thermoplasma acidophilum
4.1.1.72 4
-
4-methyl-2-oxopentanoate pH 7.0, 55°C Thermoplasma acidophilum
4.1.1.72 5
-
3-methyl-2-oxopentanoate pH 7.0, 55°C Thermoplasma acidophilum
4.1.1.72 11
-
3-methyl-2-oxobutanoate pH 7.0, 55°C Thermoplasma acidophilum