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Literature summary extracted from

  • Hicks, P.; Rinker, K.; Baker, J.; Kelly, R.
    Homomultimeric protease in the hyperthermophilic bacterium Thermotoga maritima has structural and amino acid sequence homology to bacteriocins in mesophilic bacteria (1998), FEBS Lett., 440, 393-398 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.1 EDTA 10 mM, 50% loss of activity Thermotoga maritima
3.4.21.1 SDS 1%, activity is reduced 6fold Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.1 Ca2+ proteolytic activity is 1.5fold enhanced in the presence of 10 mM Ca2+ Thermotoga maritima
3.4.21.1 Co2+ proteolytic activity is 1.4fold enhanced in the presence of 10 mM Co2+ Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.1 669000
-
 gel filtration Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.1 Thermotoga maritima Q9WZP2
-
-
3.4.21.1 Thermotoga maritima DSM 3109 Q9WZP2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.1
-
 Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.1 additional information the protease exhibits activity toward chymotrypsin and trypsin substrates. Substrate specificity to a variety of 7-amino-4-methylcoumarin-linked peptides in the order of decreasing activity: AAF, AFK, IIW, LY, GGF, AAPV. Little or no activity is detected on FVR, LLVY or GGR. No activity is noted on casein Thermotoga maritima ?
-
 ?
3.4.21.1 additional information the protease exhibits activity toward chymotrypsin and trypsin substrates. Substrate specificity to a variety of 7-amino-4-methylcoumarin-linked peptides in the order of decreasing activity: AAF, AFK, IIW, LY, GGF, AAPV. Little or no activity is detected on FVR, LLVY or GGR. No activity is noted on casein Thermotoga maritima DSM 3109 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.1 homomultimer x * 31000, the protease consists of a large globular assembly, SDS-PAGE Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.1 90
-
-
 Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.1 95
-
 half-life: 36 min Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.1 7.1
-
-
 Thermotoga maritima