Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zubimendi, J.P.; Martinatto, A.; Valacco, M.P.; Moreno, S.; Andreo, C.S.; Drincovich, M.F.; Tronconi, M.A.
    The complex allosteric and redox regulation of the fumarate hydratase and malate dehydratase reactions of Arabidopsis thaliana fumarase 1 and 2 gives clues for understanding the massive accumulation of fumarate (2018), FEBS J., 285, 2205-2224 .
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.2 0.4
-
 fumarate pH 7.7, 30°C Arabidopsis thaliana
4.2.1.2 0.5
-
 fumarate pH 7.7, 30°C Arabidopsis thaliana
4.2.1.2 0.5
-
 fumarate presence of 3 mM oxalacetate, pH 8.0, 30°C Arabidopsis thaliana
4.2.1.2 0.5
-
 L-malate presence of 3 mM oxalacetate, pH 8.0, 30°C Arabidopsis thaliana
4.2.1.2 0.5
-
 fumarate presence of 3 mM oxaloacetate, pH 8.0, 30°C Arabidopsis thaliana
4.2.1.2 0.6
-
 L-malate pH 8.2, 30°C Arabidopsis thaliana
4.2.1.2 0.6
-
 L-malate presence of 3 mM oxaloacetate, pH 8.0, 30°C Arabidopsis thaliana
4.2.1.2 0.7
-
 fumarate pH 8.0, 30°C Arabidopsis thaliana
4.2.1.2 1.8
-
 L-malate pH 8.0, 30°C Arabidopsis thaliana
4.2.1.2 1.9
-
 L-malate pH 8.2, 30°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.2 cytosol
-
 Arabidopsis thaliana 5829
-
4.2.1.2 mitochondrion
-
 Arabidopsis thaliana 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.2 195000
-
 gel filtration Arabidopsis thaliana
4.2.1.2 216000
-
 gel filtration Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.2 Arabidopsis thaliana P93033
-
-
4.2.1.2 Arabidopsis thaliana Q9FI53
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.2 fumarate + H2O
-
 Arabidopsis thaliana L-malate
-
 r
4.2.1.2 L-malate
-
 Arabidopsis thaliana fumarate + H2O
-
 r
4.2.1.2 additional information fumarate hydratase activity is favored over the malate dehydratase activity. Presence of oxalacetate, glutamine, and/or asparagine cause the malate dehydratase reaction to become preferred over the fumarate hydratase reaction Arabidopsis thaliana ?
-
 ?
4.2.1.2 additional information fumarate hydratase activity is favored over the malate dehydratase activity. Presence of oxaloacetate, glutamine, and/or asparagine cause the malate dehydratase reaction to become preferred over the fumarate hydratase reaction Arabidopsis thaliana ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.2 tetramer 4 * 58000, SDS-PAGE Arabidopsis thaliana
4.2.1.2 tetramer 4 * 54000, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
4.2.1.2 At2g47510
-
 Arabidopsis thaliana
4.2.1.2 At5g50950
-
 Arabidopsis thaliana
4.2.1.2 FUM1
-
 Arabidopsis thaliana
4.2.1.2 FUM2
-
 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.2 7.7
-
 fumarate hydratase reaction Arabidopsis thaliana
4.2.1.2 8
-
-
 Arabidopsis thaliana
4.2.1.2 8.2
-
 malate dehydratase reaction Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.2 8.5
-
 L-malate pH 8.0, 30°C Arabidopsis thaliana
4.2.1.2 8.5
-
 fumarate presence of 3 mM oxalacetate, pH 8.0, 30°C Arabidopsis thaliana
4.2.1.2 8.7
-
 L-malate pH 8.2, 30°C Arabidopsis thaliana
4.2.1.2 9
-
 L-malate pH 8.2, 30°C Arabidopsis thaliana
4.2.1.2 9.7
-
 fumarate presence of 3 mM oxaloacetate, pH 8.0, 30°C Arabidopsis thaliana
4.2.1.2 30
-
 fumarate pH 8.0, 30°C Arabidopsis thaliana
4.2.1.2 36.6
-
 fumarate pH 7.7, 30°C Arabidopsis thaliana
4.2.1.2 37
-
 L-malate presence of 3 mM oxalacetate, pH 8.0, 30°C Arabidopsis thaliana
4.2.1.2 37.8
-
 L-malate presence of 3 mM oxaloacetate, pH 8.0, 30°C Arabidopsis thaliana
4.2.1.2 57.6
-
 fumarate pH 7.7, 30°C Arabidopsis thaliana