BRENDA - Enzyme Database

Complete catalytic cycle of cofactor-independent phosphoglycerate mutase involves a spring-loaded mechanism

Roychowdhury, A.; Kundu, A.; Bose, M.; Gujar, A.; Mukherjee, S.; Das, A.; FEBS J. 282, 1097-1110 (2015)

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization
Organism
5.4.2.12
hanging drop vapor diffusion method, using 0.2 M NaCl, 0.1 M Bis/Tris pH 6.3 and 25% (w/v) polyethylene glycol 3350
Staphylococcus aureus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
5.4.2.12
Mn2+
dependent on
Staphylococcus aureus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.2.12
2-phospho-D-glycerate
Staphylococcus aureus
-
3-phospho-D-glycerate
-
-
r
5.4.2.12
2-phospho-D-glycerate
Staphylococcus aureus NCTC 8325
-
3-phospho-D-glycerate
-
-
r
5.4.2.12
3-phospho-D-glycerate
Staphylococcus aureus
-
2-phospho-D-glycerate
-
-
r
5.4.2.12
3-phospho-D-glycerate
Staphylococcus aureus NCTC 8325
-
2-phospho-D-glycerate
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.2.12
Staphylococcus aureus
Q2G029
-
-
5.4.2.12
Staphylococcus aureus NCTC 8325
Q2G029
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.4.2.12
-
Staphylococcus aureus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.12
2-phospho-D-glycerate
-
747737
Staphylococcus aureus
3-phospho-D-glycerate
-
-
-
r
5.4.2.12
2-phospho-D-glycerate
-
747737
Staphylococcus aureus NCTC 8325
3-phospho-D-glycerate
-
-
-
r
5.4.2.12
3-phospho-D-glycerate
-
747737
Staphylococcus aureus
2-phospho-D-glycerate
-
-
-
r
5.4.2.12
3-phospho-D-glycerate
-
747737
Staphylococcus aureus NCTC 8325
2-phospho-D-glycerate
-
-
-
r
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.4.2.12
additional information
the enzyme is independent from 2,3-bisphosphoglycerate
Staphylococcus aureus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.4.2.12
additional information
the enzyme is independent from 2,3-bisphosphoglycerate
Staphylococcus aureus
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
5.4.2.12
hanging drop vapor diffusion method, using 0.2 M NaCl, 0.1 M Bis/Tris pH 6.3 and 25% (w/v) polyethylene glycol 3350
Staphylococcus aureus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
5.4.2.12
Mn2+
dependent on
Staphylococcus aureus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.2.12
2-phospho-D-glycerate
Staphylococcus aureus
-
3-phospho-D-glycerate
-
-
r
5.4.2.12
2-phospho-D-glycerate
Staphylococcus aureus NCTC 8325
-
3-phospho-D-glycerate
-
-
r
5.4.2.12
3-phospho-D-glycerate
Staphylococcus aureus
-
2-phospho-D-glycerate
-
-
r
5.4.2.12
3-phospho-D-glycerate
Staphylococcus aureus NCTC 8325
-
2-phospho-D-glycerate
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.2.12
-
Staphylococcus aureus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.12
2-phospho-D-glycerate
-
747737
Staphylococcus aureus
3-phospho-D-glycerate
-
-
-
r
5.4.2.12
2-phospho-D-glycerate
-
747737
Staphylococcus aureus NCTC 8325
3-phospho-D-glycerate
-
-
-
r
5.4.2.12
3-phospho-D-glycerate
-
747737
Staphylococcus aureus
2-phospho-D-glycerate
-
-
-
r
5.4.2.12
3-phospho-D-glycerate
-
747737
Staphylococcus aureus NCTC 8325
2-phospho-D-glycerate
-
-
-
r