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Literature summary extracted from

  • Jugde, H.; Nguy, D.; Moller, I.; Cooney, J.M.; Atkinson, R.G.
    Isolation and characterization of a novel glycosyltransferase that converts phloretin to phlorizin, a potent antioxidant in apple (2008), FEBS J., 275, 3804-3814 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.357 expressed in Escherichia coli BL21-Codon-Plus-RIL cells Malus domestica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.357 0.013
-
 UDP-alpha-D-glucose at pH 7.5 and 30°C Malus domestica
2.4.1.357 0.62
-
 phloretin at pH 7.5 and 30°C Malus domestica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.357 additional information the enzyme activity is not affected by the addition of divalent cations (Mg2+ or Mn2+, 0-25 mM) or monovalent cations (Na+, 0-100 mM or K+, 0-10 mM) Malus domestica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.357 UDP-alpha-D-glucose + phloretin Malus domestica
-
 UDP + phlorizin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.357 Malus domestica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.357 His-Trap column chromatography and Superdex 200 gel filtration Malus domestica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.357 bud
-
 Malus domestica
-
2.4.1.357 flower highest expression Malus domestica
-
2.4.1.357 fruit
-
 Malus domestica
-
2.4.1.357 leaf
-
 Malus domestica
-
2.4.1.357 phloem
-
 Malus domestica
-
2.4.1.357 root
-
 Malus domestica
-
2.4.1.357 seed
-
 Malus domestica
-
2.4.1.357 shoot
-
 Malus domestica
-
2.4.1.357 xylem
-
 Malus domestica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.357 additional information no activity with caffeic acid, catechin, chlorogenic acid, 2-coumaric acid, 3-coumaric acid, 4-coumaric acid, cyanidin, 3-3,4 dihydroxyphenyl propionic acid, epicatechin, 3-hydroxybenzoic acid, naringenin, protocatechuic acid, quercetin and rutin Malus domestica ?
-
 ?
2.4.1.357 UDP-alpha-D-galactose + phloretin
-
 Malus domestica ?
-
 ?
2.4.1.357 UDP-alpha-D-glucose + phloretin
-
 Malus domestica UDP + phlorizin
-
 ?
2.4.1.357 UDP-alpha-D-xylose + phloretin
-
 Malus domestica ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.357 ? x * 53500, calculated from amino acid sequence Malus domestica
2.4.1.357 ? x * 59000, His6-tagged enzyme, SDS-PAGE Malus domestica

Synonyms

EC Number Synonyms Comment Organism
2.4.1.357 PGT1
-
 Malus domestica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.357 25
-
-
 Malus domestica

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.357 15 50
-
 Malus domestica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.357 0.0972
-
 phloretin at pH 7.5 and 30°C Malus domestica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.357 7.7
-
-
 Malus domestica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.357 7 8 the enzyme shows significant activity from pH 7.0-8.0. Activity decreases to 20% at pH 6.0 and 9.0 and less than 5% at pH 5.0 and 10.0, respectively Malus domestica

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.357 Malus domestica calculated from amino acid sequence
-
 5.8