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Literature summary extracted from

  • Kluskens, L.; Van Alebeek, G.; Walther, J.; Voragen, A.; De Vos, W.; Van Der Oost, J.
    Characterization and mode of action of an exopolygalacturonase from the hyperthermophilic bacterium Thermotoga maritima (2005), FEBS J., 272, 5464-5473 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.67 expression in Escherichia coli Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.67 0.06
-
 [(1->4)-alpha-D-galacturonide]8 pH 6.5, 80°C Thermotoga maritima
3.2.1.67 0.06
-
 [(1->4)-alpha-D-galacturonide]170 pH 6.5, 80°C Thermotoga maritima
3.2.1.67 0.07
-
 [(1->4)-alpha-D-galacturonide]7 pH 6.5, 80°C Thermotoga maritima
3.2.1.67 0.11
-
 [(1->4)-alpha-D-galacturonide]6 pH 6.5, 80°C Thermotoga maritima
3.2.1.67 0.24
-
 [(1->4)-alpha-D-galacturonide]5 pH 6.5, 80°C Thermotoga maritima
3.2.1.67 0.29
-
 [(1->4)-alpha-D-galacturonide]4 pH 6.5, 80°C Thermotoga maritima
3.2.1.67 0.34
-
 [(1->4)-alpha-D-galacturonide]2 pH 6.5, 80°C Thermotoga maritima
3.2.1.67 0.34
-
 [(1->4)-alpha-D-galacturonide]3 pH 6.5, 80°C Thermotoga maritima

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.67 intracellular
-
 Thermotoga maritima 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.67 212000
-
 gel filtration Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.67 Thermotoga maritima Q9WYR8
-
-
3.2.1.67 Thermotoga maritima DSM 3109 Q9WYR8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.67
-
 Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.67 additional information the enzyme shows low activity with xylogalacturonan Thermotoga maritima ?
-
 ?
3.2.1.67 additional information the enzyme shows low activity with xylogalacturonan Thermotoga maritima DSM 3109 ?
-
 ?
3.2.1.67 [(1->4)-alpha-D-galacturonide]170 + H2O the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA Thermotoga maritima [(1->4)-alpha-D-galacturonide]169 + D-galacturonate
-
 ?
3.2.1.67 [(1->4)-alpha-D-galacturonide]170 + H2O the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA Thermotoga maritima DSM 3109 [(1->4)-alpha-D-galacturonide]169 + D-galacturonate
-
 ?
3.2.1.67 [(1->4)-alpha-D-galacturonide]2 + H2O the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA Thermotoga maritima 2 D-galacturonate
-
 ?
3.2.1.67 [(1->4)-alpha-D-galacturonide]2 + H2O the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA Thermotoga maritima DSM 3109 2 D-galacturonate
-
 ?
3.2.1.67 [(1->4)-alpha-D-galacturonide]3 + H2O the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA Thermotoga maritima [(1->4)-alpha-D-galacturonide]2 + D-galacturonate
-
 ?
3.2.1.67 [(1->4)-alpha-D-galacturonide]3 + H2O the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA Thermotoga maritima DSM 3109 [(1->4)-alpha-D-galacturonide]2 + D-galacturonate
-
 ?
3.2.1.67 [(1->4)-alpha-D-galacturonide]4 + H2O the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA Thermotoga maritima [(1->4)-alpha-D-galacturonide]3 + D-galacturonate
-
 ?
3.2.1.67 [(1->4)-alpha-D-galacturonide]5 + H2O the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA Thermotoga maritima [(1->4)-alpha-D-galacturonide]4 + D-galacturonate
-
 ?
3.2.1.67 [(1->4)-alpha-D-galacturonide]6 + H2O the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA Thermotoga maritima [(1->4)-alpha-D-galacturonide]5 + D-galacturonate
-
 ?
3.2.1.67 [(1->4)-alpha-D-galacturonide]7 + H2O the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA Thermotoga maritima [(1->4)-alpha-D-galacturonide]6 + D-galacturonate
-
 ?
3.2.1.67 [(1->4)-alpha-D-galacturonide]8 + H2O the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA Thermotoga maritima [(1->4)-alpha-D-galacturonide]7 + D-galacturonate
-
 ?
3.2.1.67 [(1->4)-alpha-D-galacturonide]n + H2O the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA Thermotoga maritima [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate
-
 ?
3.2.1.67 [(1->4)-alpha-D-galacturonide]n + H2O the enzyme shows increasing activity on oligosaccharides with an increasing degree of polymerization. The enzyme displays specificity for saturated oligoGalpA and is unable to degrade unsaturated or methyl-esterified oligoGalpA Thermotoga maritima DSM 3109 [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.67 tetramer 4 * 50000, SDS-PAGE Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
3.2.1.67 exopolygalacturonase B
-
 Thermotoga maritima
3.2.1.67 PelB
-
 Thermotoga maritima
3.2.1.67 TM0437
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.67 80
-
-
 Thermotoga maritima

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.67 73 95 73°C: 25% of maximal activity, 95°C: about 75% of maximal activity Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.67 105
-
 melting temperature Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.67 182
-
 [(1->4)-alpha-D-galacturonide]2 pH 6.5, 80°C Thermotoga maritima
3.2.1.67 685
-
 [(1->4)-alpha-D-galacturonide]3 pH 6.5, 80°C Thermotoga maritima
3.2.1.67 821
-
 [(1->4)-alpha-D-galacturonide]6 pH 6.5, 80°C Thermotoga maritima
3.2.1.67 829
-
 [(1->4)-alpha-D-galacturonide]4 pH 6.5, 80°C Thermotoga maritima
3.2.1.67 843
-
 [(1->4)-alpha-D-galacturonide]8 pH 6.5, 80°C Thermotoga maritima
3.2.1.67 860
-
 [(1->4)-alpha-D-galacturonide]7 pH 6.5, 80°C Thermotoga maritima
3.2.1.67 934
-
 [(1->4)-alpha-D-galacturonide]5 pH 6.5, 80°C Thermotoga maritima
3.2.1.67 936
-
 [(1->4)-alpha-D-galacturonide]170 pH 6.5, 80°C Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.67 6.4
-
-
 Thermotoga maritima

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.67 5.9 6.9 pH 5.9: about 40% of maximal activity, pH 6.9: about 75% of maximal activity Thermotoga maritima

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.67 534
-
 [(1->4)-alpha-D-galacturonide]2 pH 6.5, 80°C Thermotoga maritima
3.2.1.67 2016
-
 [(1->4)-alpha-D-galacturonide]3 pH 6.5, 80°C Thermotoga maritima
3.2.1.67 2859
-
 [(1->4)-alpha-D-galacturonide]4 pH 6.5, 80°C Thermotoga maritima
3.2.1.67 3892
-
 [(1->4)-alpha-D-galacturonide]5 pH 6.5, 80°C Thermotoga maritima
3.2.1.67 7461
-
 [(1->4)-alpha-D-galacturonide]6 pH 6.5, 80°C Thermotoga maritima
3.2.1.67 12288
-
 [(1->4)-alpha-D-galacturonide]7 pH 6.5, 80°C Thermotoga maritima
3.2.1.67 14042
-
 [(1->4)-alpha-D-galacturonide]8 pH 6.5, 80°C Thermotoga maritima
3.2.1.67 15600
-
 [(1->4)-alpha-D-galacturonide]170 pH 6.5, 80°C Thermotoga maritima