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Literature summary extracted from

  • Miyazaki, K.
    A hyperthermophilic laccase from Thermus thermophilus HB27 (2005), Extremophiles, 9, 415-425 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.2 by signal peptide prediction, the enzyme is assumed to be a secretory protein starting from Gln23. The DNA encoding the mature protein is then cloned and expressed in Escherichia coli BL21 (DE3). The recombinant enzyme, expressed as an apoprotein, is dialyzed against copper-containing buffer to yield a holoprotein Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 EDTA the reaction in the presence of 0.1 mM CuSO4 is completely inhibited by the addition of 1 mM EDTA Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.9
-
2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) pH and temperature not specified in the publication Thermus thermophilus
1.10.3.2 1.88
-
syringaldazine pH and temperature not specified in the publication Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Cu2+ the enzyme requires copper ions for activity. The dependence of the activity on Cu2+ concentration is sigmoidal with the midpoint at 0.00304 mM. Other metal ions (Mg2+, Mn2+, Ca2+, Ni2+, or Zn2+ each at 1 mM) fail to support the activity. The methionine-rich region from Met271 to Met283 may be involved in copper binding Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 48986
-
calculated from sequence Thermus thermophilus
1.10.3.2 53000
-
SDS-PAGE, recombinant enzyme Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Thermus thermophilus Q72HW2
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.10.3.2 proteolytic modification by signal peptide prediction, the enzyme is assumed to be a secretory protein starting from Gln23 Thermus thermophilus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2
-
Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) + O2
-
Thermus thermophilus ?
-
?
1.10.3.2 2,6-dimethoxyphenol + O2
-
Thermus thermophilus ?
-
?
1.10.3.2 guaiacol + O2
-
Thermus thermophilus ?
-
?
1.10.3.2 syringaldazine + O2
-
Thermus thermophilus ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.10.3.2 TTC1370
-
Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 92
-
at pH 5.0, maximal activity for 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 80
-
half-life: more than 14 h Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 6.47
-
syringaldazine pH and temperature not specified in the publication Thermus thermophilus
1.10.3.2 24.6
-
2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) pH and temperature not specified in the publication Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 4.5 5.5 maximal activity for 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) and syringaldazine Thermus thermophilus

Expression

EC Number Organism Comment Expression
1.10.3.2 Thermus thermophilus copper-inducible enzyme up