EC Number | Cloned (Comment) | Organism |
---|---|---|
1.10.3.2 | by signal peptide prediction, the enzyme is assumed to be a secretory protein starting from Gln23. The DNA encoding the mature protein is then cloned and expressed in Escherichia coli BL21 (DE3). The recombinant enzyme, expressed as an apoprotein, is dialyzed against copper-containing buffer to yield a holoprotein | Thermus thermophilus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.10.3.2 | EDTA | the reaction in the presence of 0.1 mM CuSO4 is completely inhibited by the addition of 1 mM EDTA | Thermus thermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.10.3.2 | 0.9 | - |
2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) | pH and temperature not specified in the publication | Thermus thermophilus | |
1.10.3.2 | 1.88 | - |
syringaldazine | pH and temperature not specified in the publication | Thermus thermophilus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.10.3.2 | Cu2+ | the enzyme requires copper ions for activity. The dependence of the activity on Cu2+ concentration is sigmoidal with the midpoint at 0.00304 mM. Other metal ions (Mg2+, Mn2+, Ca2+, Ni2+, or Zn2+ each at 1 mM) fail to support the activity. The methionine-rich region from Met271 to Met283 may be involved in copper binding | Thermus thermophilus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 48986 | - |
calculated from sequence | Thermus thermophilus |
1.10.3.2 | 53000 | - |
SDS-PAGE, recombinant enzyme | Thermus thermophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.10.3.2 | Thermus thermophilus | Q72HW2 | - |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
1.10.3.2 | proteolytic modification | by signal peptide prediction, the enzyme is assumed to be a secretory protein starting from Gln23 | Thermus thermophilus |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.10.3.2 | - |
Thermus thermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.10.3.2 | 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) + O2 | - |
Thermus thermophilus | ? | - |
? | |
1.10.3.2 | 2,6-dimethoxyphenol + O2 | - |
Thermus thermophilus | ? | - |
? | |
1.10.3.2 | guaiacol + O2 | - |
Thermus thermophilus | ? | - |
? | |
1.10.3.2 | syringaldazine + O2 | - |
Thermus thermophilus | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.10.3.2 | TTC1370 | - |
Thermus thermophilus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 92 | - |
at pH 5.0, maximal activity for 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) | Thermus thermophilus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 80 | - |
half-life: more than 14 h | Thermus thermophilus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.10.3.2 | 6.47 | - |
syringaldazine | pH and temperature not specified in the publication | Thermus thermophilus | |
1.10.3.2 | 24.6 | - |
2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) | pH and temperature not specified in the publication | Thermus thermophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 4.5 | 5.5 | maximal activity for 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) and syringaldazine | Thermus thermophilus |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.10.3.2 | Thermus thermophilus | copper-inducible enzyme | up |