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Literature summary extracted from

  • Cool, R.; Veenstra, M.; Van Klompenburg, W.; Heyne, R.; Mueller, M.; De Vries, E.; Van Veen, H.; Konings, W.
    S-decyl-glutathione nonspecifically stimulates the ATPase activity of the nucleotide-binding domains of the human multidrug resistance-associated protein, MRP1 (ABCC1) (2002), Eur. J. Biochem., 269, 3470-3478 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.6.2.3 decyl-maltoside 1.0 mM decyl-maltoside is able to stimulate the ATPase activities of the isolated nucleotide-binding domains more than 2fold Homo sapiens
7.6.2.3 additional information vincristine (0.046 mM), vincristine (0.046 mM) plus reduced glutathione (0.5 mM), reduced glutathione (0.5 mM), oxidized glutathione (0.5 mM), probenecid (1 mM), sulfinpyranoside (1 mM), N-ethyl-maleimide-glutathione (0.1-4.0 mM), leukotriene C4 (0.1-1.6 mM), and 17beta-estradiol (0.01-0.075 mM) have no significant effect on the basal ATPase activity of the enzyme Homo sapiens
7.6.2.3 S-decylglutathione 0.2-0.25 mM S-decylglutathione is able to stimulate the ATPase activities of the isolated nucleotide-binding domains more than 2fold Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.6.2.3 expressed in Escherichia coli DH5alpha cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.6.2.3 Ca2+
-
 Homo sapiens
7.6.2.3 Co2+
-
 Homo sapiens
7.6.2.3 Decanol decanol causes a small, but significant and concentration-dependent (0.1-1.0 mM) inhibition of the ATPase activity Homo sapiens
7.6.2.3 EDTA complete inhibition at 2 mM Homo sapiens
7.6.2.3 Mn2+
-
 Homo sapiens
7.6.2.3 additional information N-ethylmaleimide does not significantly inhibit the ATPase activities at concentrations of up to 2 mM Homo sapiens
7.6.2.3 N-ethylmaleimide
-
 Homo sapiens
7.6.2.3 orthovanadate the ATPase activity of GST-tagged nucleotide-binding domains 1 and 2 is inhibited more than 40% by 2 mM orthovanadate Homo sapiens
7.6.2.3 Sodium azide the ATPase activity of GST-tagged nucleotide-binding domains 1 and 2 is inhibited more than 70% by 2 mM sodium azide Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.6.2.3 0.34
-
 ATP GST-fused form of nucleotide-binding domain 1 of the enzyme MRP1, at pH 7.5 and 30°C Homo sapiens
7.6.2.3 0.91
-
 ATP GST-fused form of nucleotide-binding domain 2 of the enzyme MRP1, at pH 7.5 and 30°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.6.2.3 Mg2+ 100% activity in the presence of 2 mM Mg2+ Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
7.6.2.3 Homo sapiens P33527
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.6.2.3 glutathione-Sepharose column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.6.2.3 ATP + H2O + S-decylglutathione[side 1]
-
 Homo sapiens ADP + phosphate + S-decylglutathione[side 2]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.6.2.3 ABCC1
-
 Homo sapiens
7.6.2.3 MRP1
-
 Homo sapiens
7.6.2.3 multidrug resistance-associated protein
-
 Homo sapiens