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Literature summary extracted from

  • Ryu, S.; Kim, J.; Huong, N.; Woo, E.; Moon, S.; Lee, S.
    Molecular cloning and characterization of trehalose synthase from Thermotoga maritima DSM3109 Syntheses of trehalose disaccharide analogues and NDP-glucoses (2010), Enzyme Microb. Technol., 47, 249-256 .
No PubMed abstract available

Application

EC Number Application Comment Organism
2.4.1.245 synthesis the enzyme is a useful thermostable enzyme particularly for the production of the trehalose analogue containing a mannose that acts as the inhibitor of intestinal sucrase Thermotoga maritima

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.245 expressed in Escherichia coli Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.245 Ca2+ 5 mM, 35% loss of activity Thermotoga maritima
2.4.1.245 dimethylformamide 5 mM, 79% loss of activity Thermotoga maritima
2.4.1.245 DMSO 5 mM, 89% loss of activity Thermotoga maritima
2.4.1.245 ethanol 5 mM, 47% loss of activity Thermotoga maritima
2.4.1.245 methanol 5 mM, 36% loss of activity Thermotoga maritima
2.4.1.245 Mn2+ 5 mM, 82% loss of activity Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.245 1.7
-
 UDP-alpha-D-glucose pH 6.0, 60°C Thermotoga maritima
2.4.1.245 1.7
-
 GDP-alpha-D-glucose pH 6.0, 60°C Thermotoga maritima
2.4.1.245 2.3
-
 ADP-alpha-D-glucose pH 6.0, 60°C Thermotoga maritima
2.4.1.245 40
-
 ADP pH 6.0, 60°C Thermotoga maritima
2.4.1.245 54
-
 GDP pH 6.0, 60°C Thermotoga maritima
2.4.1.245 71
-
 UDP pH 6.0, 60°C Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.245 Mn2+ 5 mM, 1.2fold activation Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.245 47000
-
 SDS-PAGE Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.245 Thermotoga maritima
-
-
-
2.4.1.245 Thermotoga maritima DSM 3109
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.245
-
 Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.245 ADP-alpha-D-glucose + D-glucose
-
 Thermotoga maritima alpha,alpha-trehalose + ADP
-
 r
2.4.1.245 ADP-alpha-D-glucose + D-glucose
-
 Thermotoga maritima DSM 3109 alpha,alpha-trehalose + ADP
-
 r
2.4.1.245 alpha,alpha-trehalose + ADP both soluble and glutathione-S-transferase-fusion enzymes have the greatest preference for ADP as the acceptor substrate in the synthesis of NDP-Glc, and they also employ GDP, UDP, and CDP as less favorable substrates, approximately in this order Thermotoga maritima ADP-alpha-D-glucose + D-glucose
-
 r
2.4.1.245 alpha,alpha-trehalose + ADP both soluble and glutathione-S-transferase-fusion enzymes have the greatest preference for ADP as the acceptor substrate in the synthesis of NDP-Glc, and they also employ GDP, UDP, and CDP as less favorable substrates, approximately in this order Thermotoga maritima DSM 3109 ADP-alpha-D-glucose + D-glucose
-
 r
2.4.1.245 alpha,alpha-trehalose + CDP both soluble and glutathione-S-transferase-fusion enzymes have the greatest preference for ADP as the acceptor substrate in the synthesis of NDP-Glc, and they also employ GDP, UDP, and CDP as less favorable substrates, approximately in this order Thermotoga maritima CDP-alpha-D-glucose + D-glucose
-
 r
2.4.1.245 alpha,alpha-trehalose + GDP both soluble and glutathione-S-transferase-fusion enzymes have the greatest preference for ADP as the acceptor substrate in the synthesis of NDP-Glc, and they also employ GDP, UDP, and CDP as less favorable substrates, approximately in this order Thermotoga maritima GDP-alpha-D-glucose + D-glucose
-
 ?
2.4.1.245 alpha,alpha-trehalose + GDP both soluble and glutathione-S-transferase-fusion enzymes have the greatest preference for ADP as the acceptor substrate in the synthesis of NDP-Glc, and they also employ GDP, UDP, and CDP as less favorable substrates, approximately in this order Thermotoga maritima DSM 3109 GDP-alpha-D-glucose + D-glucose
-
 ?
2.4.1.245 alpha,alpha-trehalose + UDP both soluble and glutathione-S-transferase-fusion enzymes have the greatest preference for ADP as the acceptor substrate in the synthesis of NDP-Glc, and they also employ GDP, UDP, and CDP as less favorable substrates, approximately in this order Thermotoga maritima UDP-alpha-D-glucose + D-glucose
-
 ?
2.4.1.245 CDP-alpha-D-glucose + D-glucose
-
 Thermotoga maritima alpha,alpha-trehalose + CDP
-
 r
2.4.1.245 GDP-alpha-D-glucose + D-glucose
-
 Thermotoga maritima alpha,alpha-trehalose + GDP
-
 r
2.4.1.245 additional information the enzyme does not utilize galactose as an acceptor Thermotoga maritima ?
-
 ?
2.4.1.245 additional information the enzyme does not utilize galactose as an acceptor Thermotoga maritima DSM 3109 ?
-
 ?
2.4.1.245 UDP-alpha-D-glucose + D-fructose the enzyme employs a glucose acceptor with the highest preference and other monosaccharides (D-fructose, D-mannose) with less affinities acceptors. The trehalose analogue containing mannose is produced with approximately 70% of trehalose production and a two-fold greater amount than the analogue containing a fructose for the 12-24 h reaction Thermotoga maritima ?
-
 r
2.4.1.245 UDP-alpha-D-glucose + D-glucose the purified soluble enzyme, as well as the glutathione-S-transferase-fusion enzyme, show the catalytic specificity for synthesizing trehalose from UDP-alpha-D-glucose as the donor and glucose as the acceptor. The enzyme employs a glucose acceptor with the highest preference and other monosaccharides (D-fructose, D-mannose) with less affinities acceptors. Both soluble and glutathione-S-transferase-fusion enzymes have the greatest preference for ADP as the acceptor substrate in the synthesis of NDP-Glc, and they also employ GDP, UDP, and CDP as less favorable substrates, approximately in this order Thermotoga maritima alpha,alpha-trehalose + UDP
-
 r
2.4.1.245 UDP-alpha-D-glucose + D-mannose the enzyme employs a glucose acceptor with the highest preference and other monosaccharides (D-fructose, D-mannose) with less affinities acceptors. The trehalose analogue containing mannose is produced with approximately 70% of trehalose production and a two-fold greater amount than the analogue containing a fructose for the 12-24 h reaction Thermotoga maritima ?
-
 r
2.4.1.245 UDP-alpha-D-glucose + D-mannose the enzyme employs a glucose acceptor with the highest preference and other monosaccharides (D-fructose, D-mannose) with less affinities acceptors. The trehalose analogue containing mannose is produced with approximately 70% of trehalose production and a two-fold greater amount than the analogue containing a fructose for the 12-24 h reaction Thermotoga maritima DSM 3109 ?
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.245 monomer 1 * 47000, SDS-PAGE Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
2.4.1.245 TmTreT
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.245 65
-
-
 Thermotoga maritima

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.245 35 70 35°C: about 40% of maximal activity, 70°C: about 70% of maximal activity Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.245 50
-
 2 h, activity of the the glutathione-S-transferase-fusion enzyme is fully maintained Thermotoga maritima
2.4.1.245 60
-
 2 h, 10% decrease of the activity of the the glutathione-S-transferase-fusion enzyme, activity of the soluble enzyme is decreased by 35% Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.245 7.1
-
 UDP pH 6.0, 60°C Thermotoga maritima
2.4.1.245 9.7
-
 GDP pH 6.0, 60°C Thermotoga maritima
2.4.1.245 11.1
-
 ADP pH 6.0, 60°C Thermotoga maritima
2.4.1.245 20
-
 ADP-alpha-D-glucose pH 6.0, 60°C Thermotoga maritima
2.4.1.245 25
-
 UDP-alpha-D-glucose pH 6.0, 60°C Thermotoga maritima
2.4.1.245 34
-
 GDP-alpha-D-glucose pH 6.0, 60°C Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.245 6
-
-
 Thermotoga maritima

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.245 4.5 8 pH 4.5: about 45% of maximal activity, pH 8.0: about 40% of maximal activity Thermotoga maritima

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.245 5 10 activity is relatively stable in the wide pH range of 5.0-10.0 Thermotoga maritima

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.245 0.1
-
 UDP pH 6.0, 60°C Thermotoga maritima
2.4.1.245 0.2
-
 GDP pH 6.0, 60°C Thermotoga maritima
2.4.1.245 0.3
-
 ADP pH 6.0, 60°C Thermotoga maritima
2.4.1.245 9
-
 ADP-alpha-D-glucose pH 6.0, 60°C Thermotoga maritima
2.4.1.245 15
-
 UDP-alpha-D-glucose pH 6.0, 60°C Thermotoga maritima
2.4.1.245 20
-
 GDP-alpha-D-glucose pH 6.0, 60°C Thermotoga maritima