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Literature summary extracted from

  • Lu, Y.; Wang, L.; Teng, F.; Zhang, J.; Hu, M.; Tao, Y.
    Production of myo-inositol from glucose by a novel trienzymatic cascade of polyphosphate glucokinase, inositol 1-phosphate synthase and inositol monophosphatase (2018), Enzyme Microb. Technol., 112, 1-5 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.1.63 synthesis production of myo-inositol from glucose by a novel trienzymatic cascade of polyphosphate glucokinase, inositol 1-phosphate synthase and inositol monophosphatase. myo-Inositol (inositol) is important in the cosmetics, pharmaceutical and functional food industries. The conversion ratio from glucose to inositol reaches 90%, which is promising for the enzymatic synthesis of inositol without ATP supplementation Arthrobacter sp.
3.1.3.25 synthesis construction of a pathway to produce inositol from glucose using polyphosphate glucokinase PPGK from Arthrobacter sp. OY3WO11, inositol 1-phosphate synthase IPS from Trypanosoma brucei TREU927 and inositol monophosphatase IMP Escherichia coli, all expressed in Escherichia coli. The conversion ratio from glucose to inositol reaches 90% Escherichia coli
3.1.3.25 synthesis production of myo-inositol from glucose by a novel trienzymatic cascade of polyphosphate glucokinase, inositol 1-phosphate synthase and inositol monophosphatase. myo-Inositol (inositol) is important in the cosmetics, pharmaceutical and functional food industries. The conversion ratio from glucose to inositol reaches 90%, which is promising for the enzymatic synthesis of inositol without ATP supplementation Escherichia coli
5.5.1.4 synthesis production of myo-inositol from glucose by a novel trienzymatic cascade of polyphosphate glucokinase, inositol 1-phosphate synthase and inositol monophosphatase. myo-Inositol (inositol) is important in the cosmetics, pharmaceutical and functional food industries. The conversion ratio from glucose to inositol reaches 90%, which is promising for the enzymatic synthesis of inositol without ATP supplementation Trypanosoma brucei

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.63
-
 Arthrobacter sp.
3.1.3.25
-
 Escherichia coli
5.5.1.4
-
 Trypanosoma brucei
5.5.1.4 expressed in Escherichia coli Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.5.1.4 additional information not inhibited by up to 50 mM D-glucose Trypanosoma brucei
5.5.1.4 phosphate the enzyme activity is almost completely abolished at 125 mM Trypanosoma brucei
5.5.1.4 sodium hexametaphosphate the enzyme activity is almost completely abolished at 20 mM Trypanosoma brucei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.5.1.4 D-Glucose 6-phosphate Trypanosoma brucei
-
 L-myo-Inositol 1-phosphate
-
 ir
5.5.1.4 D-Glucose 6-phosphate Trypanosoma brucei TREU927
-
 L-myo-Inositol 1-phosphate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.63 Arthrobacter sp. A0A176UHL5
-
-
2.7.1.63 Arthrobacter sp. OY3WO11 A0A176UHL5
-
-
3.1.3.25 Escherichia coli
-
-
-
5.5.1.4 Trypanosoma brucei
-
-
-
5.5.1.4 Trypanosoma brucei Q38AQ2
-
-
5.5.1.4 Trypanosoma brucei TREU927
-
-
-
5.5.1.4 Trypanosoma brucei TREU927 Q38AQ2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.63
-
 Arthrobacter sp.
3.1.3.25
-
 Escherichia coli
5.5.1.4
-
 Trypanosoma brucei
5.5.1.4 Ni-NTA column chromatography Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.5.1.4 D-Glucose 6-phosphate
-
 Trypanosoma brucei L-myo-Inositol 1-phosphate
-
 ir
5.5.1.4 D-Glucose 6-phosphate
-
 Trypanosoma brucei TREU927 L-myo-Inositol 1-phosphate
-
 ir

Synonyms

EC Number Synonyms Comment Organism
2.7.1.63 polyphosphate glucokinase
-
 Arthrobacter sp.
2.7.1.63 PPGK
-
 Arthrobacter sp.
3.1.3.25 IMP
-
 Escherichia coli
3.1.3.25 inositol monophsphatase
-
 Escherichia coli
5.5.1.4 Inositol 1-phosphate synthase
-
 Trypanosoma brucei
5.5.1.4 IPS
-
 Trypanosoma brucei

Cofactor

EC Number Cofactor Comment Organism Structure
5.5.1.4 NAD+ required Trypanosoma brucei

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
5.5.1.4 15
-
 at pH 7.0 and 37°C Trypanosoma brucei sodium hexametaphosphate
5.5.1.4 50
-
 at pH 7.0 and 37°C Trypanosoma brucei phosphate