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Literature summary extracted from

  • Joly, N.; Martino, L.; Gigant, E.; Dumont, J.; Pintard, L.
    Microtubule-severing activity of the AAA+ ATPase Katanin is essential for female meiotic spindle assembly (2016), Development, 143, 3604-3614 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.1.1 expression in Escherichia coli BL21 (DE3) Caenorhabditis elegans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.6.1.1 1.45
-
 ATP pH 8.0, 28°C, MEI-1 WT/MEI-2 wild-type enzyme Caenorhabditis elegans
5.6.1.1 2.19
-
 ATP pH 8.0, 28°C, mutant enzyme MEI-1 K293A/MEI-2 Caenorhabditis elegans
5.6.1.1 2.28
-
 ATP pH 8.0, 28°C, mutant enzyme MEI-1 K293Q/MEI-2 Caenorhabditis elegans
5.6.1.1 2.94
-
 ATP pH 8.0, 28°C, mutant enzyme MEI-1 K239A/MEI-2 Caenorhabditis elegans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.6.1.1 ATP + H2O + microtubule Caenorhabditis elegans assembly of female meiotic spindles requires MEI-1 and MEI-2, which constitute the microtubule-severing AAA+ ATPase Katanin ADP + phosphate + alpha/beta tubulin heterodimers
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.6.1.1 Caenorhabditis elegans P34808
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.1.1
-
 Caenorhabditis elegans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.1.1 ATP + H2O + microtubule
-
 Caenorhabditis elegans ADP + phosphate + alpha/beta tubulin heterodimers
-
 ?
5.6.1.1 ATP + H2O + microtubule assembly of female meiotic spindles requires MEI-1 and MEI-2, which constitute the microtubule-severing AAA+ ATPase Katanin Caenorhabditis elegans ADP + phosphate + alpha/beta tubulin heterodimers
-
 ?

Subunits

EC Number Subunits Comment Organism
5.6.1.1 hexamer MEI-2 subunit stabilizes the MEI-1 hexamer for optimal ATP-dependent microtubule severing Caenorhabditis elegans

Synonyms

EC Number Synonyms Comment Organism
5.6.1.1 katanin
-
 Caenorhabditis elegans

General Information

EC Number General Information Comment Organism
5.6.1.1 malfunction variants show disorganized microtubules that lack focused spindle poles reminiscent of the Katanin loss-of-function phenotype, demonstrating that the microtubule-severing activity is essential for meiotic spindle assembly in Caenorhabditis elegans Caenorhabditis elegans
5.6.1.1 physiological function assembly of female meiotic spindles requires MEI-1 and MEI-2, which constitute the microtubule-severing AAA+ ATPase Katanin Caenorhabditis elegans