Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kotb, E.
    Production, purification and partial characterization for an oxalate decarboxylase (OxDcase) from the probiote Lactobacillus plantarum KSK-II (2018), Curr. Sci., 114, 835-844 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.2 arabic gum about 120% activity at 0.1% (w/v) Lactiplantibacillus plantarum
4.1.1.2 arabinogalactan about 150% activity at 0.1% (w/v) Lactiplantibacillus plantarum
4.1.1.2 ferrocyanide about 112% activity at 5 mM Lactiplantibacillus plantarum
4.1.1.2 fructooligosaccharide about 160% activity at 0.1% (w/v) Lactiplantibacillus plantarum
4.1.1.2 guar gum about 140% activity at 0.1% (w/v) Lactiplantibacillus plantarum
4.1.1.2 inulin about 110% activity at 0.1% (w/v) Lactiplantibacillus plantarum
4.1.1.2 L-ascorbate about 110% activity at 5 mM Lactiplantibacillus plantarum
4.1.1.2 lactitol about 130% activity at 0.1% (w/v) Lactiplantibacillus plantarum
4.1.1.2 maltodextrin about 145% activity at 0.1% (w/v) Lactiplantibacillus plantarum
4.1.1.2 o-phenylenediamine about 138% activity at 5 mM Lactiplantibacillus plantarum
4.1.1.2 Triton X-100 about 115% activity at 5 mM Lactiplantibacillus plantarum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.2 2-oxoglutaric acid about 82% residual activity at 25 mM Lactiplantibacillus plantarum
4.1.1.2 Citric acid about 77% residual activity at 25 mM Lactiplantibacillus plantarum
4.1.1.2 Fumaric acid about 73% residual activity at 25 mM Lactiplantibacillus plantarum
4.1.1.2 hydroxylamine about 50% residual activity at 5 mM Lactiplantibacillus plantarum
4.1.1.2 Maleic acid about 69% residual activity at 25 mM Lactiplantibacillus plantarum
4.1.1.2 malonic acid about 68% residual activity at 25 mM Lactiplantibacillus plantarum
4.1.1.2 NaBr about 71% residual activity at 50 mM Lactiplantibacillus plantarum
4.1.1.2 NaCl about 98% residual activity at 50 mM Lactiplantibacillus plantarum
4.1.1.2 NaF about 68% residual activity at 50 mM Lactiplantibacillus plantarum
4.1.1.2 NaI about 82% residual activity at 50 mM Lactiplantibacillus plantarum
4.1.1.2 oxaloacetic acid about 68% residual activity at 25 mM Lactiplantibacillus plantarum
4.1.1.2 Phthalic acid about 97% residual activity at 25 mM Lactiplantibacillus plantarum
4.1.1.2 Sodium dithionite about 75% residual activity at 5 mM Lactiplantibacillus plantarum
4.1.1.2 sodium sulfite about 60% residual activity at 5 mM Lactiplantibacillus plantarum
4.1.1.2 succinic acid about 77% residual activity at 25 mM Lactiplantibacillus plantarum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.2 12.7
-
 oxalate at pH 5.0 and 35°C Lactiplantibacillus plantarum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.2 oxalate + H+ Lactiplantibacillus plantarum
-
 formate + CO2
-
 ?
4.1.1.2 oxalate + H+ Lactiplantibacillus plantarum KSK-II
-
 formate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.2 Lactiplantibacillus plantarum
-
-
-
4.1.1.2 Lactiplantibacillus plantarum KSK-II
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.2 DEAE-Sepharose column chromatography and Sephadex G-100 gel filtration Lactiplantibacillus plantarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.2 oxalate + H+
-
 Lactiplantibacillus plantarum formate + CO2
-
 ?
4.1.1.2 oxalate + H+
-
 Lactiplantibacillus plantarum KSK-II formate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.2 ? x * 63000, SDS-PAGE Lactiplantibacillus plantarum

Synonyms

EC Number Synonyms Comment Organism
4.1.1.2 OxDcase
-
 Lactiplantibacillus plantarum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.2 35
-
-
 Lactiplantibacillus plantarum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.1.2 20 50 more than 70% activity between 20 and 50°C Lactiplantibacillus plantarum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.2 47
-
 the enzyme is stable at pH 4.0-7.0 for 1 h Lactiplantibacillus plantarum
4.1.1.2 70
-
 the enzyme is maximally stable till 70?C for 1 h Lactiplantibacillus plantarum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.2 64
-
 oxalate at pH 5.0 and 35°C Lactiplantibacillus plantarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.2 5
-
-
 Lactiplantibacillus plantarum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.2 4 6 more than 70% activity between pH 4.0 and 6.0 Lactiplantibacillus plantarum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.1.1.2 Lactiplantibacillus plantarum isoelectric focusing
-
 3.5

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.1.2 5.05
-
 oxalate at pH 5.0 and 35°C Lactiplantibacillus plantarum