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Literature summary extracted from

  • Syren, P.O.; Henche, S.; Eichler, A.; Nestl, B.M.; Hauer, B.
    Squalene-hopene cyclases-evolution, dynamics and catalytic scope (2016), Curr. Opin. Struct. Biol., 41, 73-82 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
5.4.99.17 D376C inactive Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
5.4.99.17 F365C the mutant is able to convert geraniol into (S)-gamma-cyclogeraniol and cyclogeraniol hydrate Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
5.4.99.17 G600F the mutant is able to convert geraniol into (S)-gamma-cyclogeraniol and cyclogeraniol hydrate Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
5.4.99.17 I261A the mutant exclusively converts (S)-citronellal to ( -)-iso-isopulegol isomer (1R,2S,5S)-5-methyl-2-(prop-1-en-2-yl)cyclohexan-1-ol with an 11times improved activity as compared to the wild type enzyme Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
5.4.99.17 L607F the mutant produces the farnesyl ether with 6times higher yield from farnesol than the wild type enzyme Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
5.4.99.17 Y420W/G600F the mutant produces selectively (1S)-3-(hydroxymethyl)-2,2-dimethyl-4-methylidenecyclohexan-1-ol from 6,7-epoxygeraniol with a conversion of up to 78% Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.99.17 squalene Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
-
 hop-22(29)-ene
-
 ?
5.4.99.17 squalene + H2O Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
-
 hopan-22-ol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.17 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 P33247
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.17 (2E,6E)-farnesol + H2O
-
 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 [(1S,4aS,8aS)-5,5,8a-trimethyl-2-methylidenedecahydronaphthalen-1-yl]methanol + [(1S,4aS,8aS)-2,5,5,8a-tetramethyl-1,4,4a,5,6,7,8,8a-octahydronaphthalen-1-yl]methanol + (1S,2R,4aS,8aS)-1-(hydroxymethyl)-2,5,5,8a-tetramethyldecahydronaphthalen-2-ol + (1S,2R,4aS,8aS)-1-(hydroxymethyl)-2-O-((2E,6E)-farnesyl)-2,5,5,8a-tetramethyldecahydronaphthalen-2-ol
-
 ?
5.4.99.17 6,7-epoxygeraniol
-
 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 ?
-
 ?
5.4.99.17 beta-pinene + H2O
-
 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 alpha-pinene + terpinolene + 1-methyl-4-(propan-2-yl)cyclohexa-1,3-diene + camphene + 2-(4-methylcyclohexa-1,3-dien-1-yl)propan-2-ol + limonene
-
 ?
5.4.99.17 citronellal + H2O
-
 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 isopulegol + neo-isopulegol + iso-isopulegol
-
 ?
5.4.99.17 additional information no activity with geraniol Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 ?
-
 ?
5.4.99.17 squalene
-
 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 hop-22(29)-ene
-
 ?
5.4.99.17 squalene + H2O
-
 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446 hopan-22-ol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.4.99.17 SHC
-
 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
5.4.99.17 squalene-hopene cyclase
-
 Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446