EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.99.12 | gene ribB, overexpression in Escherichia coli wild-type strain RF01 | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.1.99.12 | additional information | engineering of Escherichia coli for increased riboflavin production: overexpression of gene ribB to increase the flux from ribulose 5-phosphate to 3,4-dihydroxybutan-2-one 4-phosphate. Then ndk and gmk genes are overexpressed to enhance GTP supply. Subsequently, a R419L mutation is introduced into purA to reduce the flux from IMP to AMP. Co-overexpression of mutant purF and prs genes further increased riboflavin production. The final strain RF18S produces 387.6 mg riboflavin per liter with a yield of 44.8 mg riboflavin per gram glucose in shake-flask fermentations. The final titer and yield are 72.2% and 55.6%. Mutant strain and method evaluation, overview | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.99.12 | D-ribulose 5-phosphate | Escherichia coli | - |
formate + L-3,4-dihydroxybutan-2-one 4-phosphate | - |
? | |
4.1.99.12 | D-ribulose 5-phosphate | Escherichia coli K-12 / MG1655 | - |
formate + L-3,4-dihydroxybutan-2-one 4-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.99.12 | Escherichia coli | P0A7J0 | - |
- |
4.1.99.12 | Escherichia coli K-12 / MG1655 | P0A7J0 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.99.12 | D-ribulose 5-phosphate | - |
Escherichia coli | formate + L-3,4-dihydroxybutan-2-one 4-phosphate | - |
? | |
4.1.99.12 | D-ribulose 5-phosphate | - |
Escherichia coli K-12 / MG1655 | formate + L-3,4-dihydroxybutan-2-one 4-phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.99.12 | DHBP synthase | - |
Escherichia coli |
4.1.99.12 | ribB | - |
Escherichia coli |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.1.99.12 | physiological function | 3,4-dihydroxy-2-butanone 4-phosphate (DHBP) and GTP are the precursors for riboflavin biosynthesis | Escherichia coli |