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Literature summary extracted from

  • Sutiono, S.; Carsten, J.; Sieber, V.
    Structure-guided engineering of alpha-keto acid decarboxylase for the production of higher alcohols at elevated temperature (2018), ChemSusChem, 11, 3335-3344 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.72 V461I 3.3 degrees increase in thermostability compared to wild-type Lactococcus lactis
4.1.1.72 V461I/A290M increase in thermostability compared to wild-type Lactococcus lactis
4.1.1.72 V461I/A290M/Q252N/D306G/E316R/F388Y increase in thermostability compared to wild-type Lactococcus lactis
4.1.1.72 V461I/A290M/Q252N/D306G/E316R/F388Y/I404C increase in thermostability compared to wild-type Lactococcus lactis
4.1.1.72 V461I/A290M/Q252N/D306G/E316R/F388Y/L434C increase in thermostability compared to wild-type Lactococcus lactis
4.1.1.72 V461I/A290M/Q252N/D306G/E316R/F388Y/L434C/M290C greater than 400fold improvement of half-life at 70°C and greater than 600fold increase in process stability in the presence of 4% isobutanol at 50°C Lactococcus lactis
4.1.1.72 V461I/A290M/Q252N/D306G/E316R/F388Y/L434C/M290V increase in thermostability compared to wild-type Lactococcus lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.72 2.6
-
3-methyl-2-oxobutanoate mutant V461I/A290M/Q252N/D306G/E316R/F388Y/I404C, pH 6.5, 30°C Lactococcus lactis
4.1.1.72 5.1
-
3-methyl-2-oxobutanoate wild-type, pH 6.5, 30°C Lactococcus lactis
4.1.1.72 5.2
-
3-methyl-2-oxobutanoate mutant V461I/A290M/Q252N/D306G/E316R/F388Y/L434C/M290C, pH 6.5, 30°C Lactococcus lactis
4.1.1.72 5.6
-
3-methyl-2-oxobutanoate mutant V461I, pH 6.5, 30°C Lactococcus lactis
4.1.1.72 6.6
-
3-methyl-2-oxobutanoate mutant V461I/A290M/Q252N/D306G/E316R/F388Y/L434C/M290V, pH 6.5, 30°C Lactococcus lactis
4.1.1.72 8.6
-
3-methyl-2-oxobutanoate mutant A290M/Q252N/D306G/E316R/F388Y/L434C, pH 6.5, 30°C Lactococcus lactis
4.1.1.72 9.3
-
3-methyl-2-oxobutanoate mutant V461I/A290M, pH 6.5, 30°C Lactococcus lactis
4.1.1.72 14.6
-
3-methyl-2-oxobutanoate mutant V461I/A290M/Q252N/D306G/E316R/F388Y, pH 6.5, 30°C Lactococcus lactis

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.72 Lactococcus lactis Q6QBS4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.72 3-methyl-2-oxobutanoate
-
Lactococcus lactis 2-methylpropanal + CO2
-
?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.72 KdcA
-
Lactococcus lactis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.72 61
-
wild-type, melting temperature Lactococcus lactis
4.1.1.72 64
-
mutant V461I, melting temperature Lactococcus lactis
4.1.1.72 66.5
-
mutant V461I/A290M, melting temperature Lactococcus lactis
4.1.1.72 70.5
-
mutant V461I/A290M/Q252N/D306G/E316R/F388Y, melting temperature Lactococcus lactis
4.1.1.72 74.5
-
mutant V461I/A290M/Q252N/D306G/E316R/F388Y/L434C, melting temperature Lactococcus lactis
4.1.1.72 74.5
-
mutant V461I/A290M/Q252N/D306G/E316R/F388Y/L434C/M290C, melting temperature Lactococcus lactis
4.1.1.72 74.5
-
mutant V461I/A290M/Q252N/D306G/E316R/F388Y/L434C/M290V, melting temperature Lactococcus lactis
4.1.1.72 75.5
-
mutant V461I/A290M/Q252N/D306G/E316R/F388Y/I404C, melting temperature Lactococcus lactis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.72 5.2
-
3-methyl-2-oxobutanoate mutant V461I/A290M/Q252N/D306G/E316R/F388Y/I404C, pH 6.5, 30°C Lactococcus lactis
4.1.1.72 43
-
3-methyl-2-oxobutanoate mutant V461I/A290M/Q252N/D306G/E316R/F388Y/L434C/M290V, pH 6.5, 30°C Lactococcus lactis
4.1.1.72 69
-
3-methyl-2-oxobutanoate mutant V461I/A290M/Q252N/D306G/E316R/F388Y/L434C/M290C, pH 6.5, 30°C Lactococcus lactis
4.1.1.72 95
-
3-methyl-2-oxobutanoate mutant A290M/Q252N/D306G/E316R/F388Y/L434C, pH 6.5, 30°C Lactococcus lactis
4.1.1.72 118
-
3-methyl-2-oxobutanoate mutant V461I/A290M, pH 6.5, 30°C Lactococcus lactis
4.1.1.72 120
-
3-methyl-2-oxobutanoate mutant V461I, pH 6.5, 30°C Lactococcus lactis
4.1.1.72 145
-
3-methyl-2-oxobutanoate wild-type, pH 6.5, 30°C Lactococcus lactis
4.1.1.72 149
-
3-methyl-2-oxobutanoate mutant V461I/A290M/Q252N/D306G/E316R/F388Y, pH 6.5, 30°C Lactococcus lactis