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Literature summary extracted from

  • Zhang, X.; Chung, L.W.
    Alternative mechanistic strategy for enzyme catalysis in a Ni-dependent lactate racemase (LarA) intermediate destabilization by the cofactor (2017), Chemistry, 23, 3623-3630 .
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.1.2.1 Ni2+ required, the Ni-tethered pincer cofactor in enzyme LarA increases reaction barriers and destabilizes NADH-like pyruvate intermediates, due to the less electrophilic Ni cofactor and to the ring strain in the pyruvate intermediates, the Ni ion decreases the electron affinity of cofactor Lactiplantibacillus plantarum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.2.1 (S)-lactate Lactiplantibacillus plantarum
-
 (R)-lactate
-
 r
5.1.2.1 (S)-lactate Lactiplantibacillus plantarum ATCC BAA-793 / NCIMB 8826 / WCFS1
-
 (R)-lactate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.1.2.1 Lactiplantibacillus plantarum F9USS9
-
-
5.1.2.1 Lactiplantibacillus plantarum ATCC BAA-793 / NCIMB 8826 / WCFS1 F9USS9
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
5.1.2.1 (S)-Lactate = (R)-lactate the Ni-tethered pincer cofactor in enzyme LarA increases reaction barriers and destabilizes NADH-like pyruvate intermediates, due to the less electrophilic Ni cofactor and to the ring strain in the pyruvate intermediates, reaction mechanism, active and transition state structure, modeling Lactiplantibacillus plantarum
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.2.1 (S)-lactate
-
 Lactiplantibacillus plantarum (R)-lactate
-
 r
5.1.2.1 (S)-lactate
-
 Lactiplantibacillus plantarum ATCC BAA-793 / NCIMB 8826 / WCFS1 (R)-lactate
-
 r

Synonyms

EC Number Synonyms Comment Organism
5.1.2.1 LARa
-
 Lactiplantibacillus plantarum

General Information

EC Number General Information Comment Organism
5.1.2.1 additional information the active-site structure is constructed from chain B of the recent crystal structure of Lactobacillus plantarum LarA, PDB ID 5HUQ Lactiplantibacillus plantarum