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Literature summary extracted from
- Rhodococcus erythropolis oleate hydratase a new member in the oleate hydratase family tree - biochemical and structural studies (2018), ChemCatChem, 10, 407-414 .
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.1.53 | expression in Escherichia coli | Rhodococcus erythropolis |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 4.2.1.53 | sitting-drop vapor-diffusion method | Rhodococcus erythropolis |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.2.1.53 | M77E | strong decrease (81%) in the conversion efficiency from oleic acid to 10-hydroxystearic acid | Rhodococcus erythropolis |
| 4.2.1.53 | V393T | strong decrease (90%) in the conversion efficiency from oleic acid to 10-hydroxystearic acid | Rhodococcus erythropolis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.53 | 0.49 | - |
oleate | pH 7.2, 28°C | Rhodococcus erythropolis |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.53 | Mg2+ | crystal structure reveals a coordinated Mg2+ ion close to the putative active site of the enzyme. Experimental data show that the Mg2+ ion has no influence on the hydration activity | Rhodococcus erythropolis | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.53 | Rhodococcus erythropolis | T5I9M6 | - |
- |
| 4.2.1.53 | Rhodococcus erythropolis DN1 | T5I9M6 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.53 | - |
Rhodococcus erythropolis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.53 | 0.15 | - |
alpha-linolenic acid, pH 7.2, 28°C | Rhodococcus erythropolis |
| 4.2.1.53 | 0.156 | - |
linoleic acid, pH 7.2, 28°C | Rhodococcus erythropolis |
| 4.2.1.53 | 0.615 | - |
gamma-linolenic acid, pH 7.2, 28°C | Rhodococcus erythropolis |
| 4.2.1.53 | 1.205 | - |
palmitoleic acid, pH 7.2, 28°C | Rhodococcus erythropolis |
| 4.2.1.53 | 1.266 | - |
oleate, pH 7.2, 28°C | Rhodococcus erythropolis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.53 | (6Z,9Z,12Z)-octadec-6,9,12-trienoic acid + H2O | - |
Rhodococcus erythropolis | (6Z,12Z)-10-hydroxyoctadec-6,12-dienoic acid | - |
? | |
| 4.2.1.53 | (6Z,9Z,12Z)-octadec-6,9,12-trienoic acid + H2O | - |
Rhodococcus erythropolis DN1 | (6Z,12Z)-10-hydroxyoctadec-6,12-dienoic acid | - |
? | |
| 4.2.1.53 | (9Z,12Z)-octadec-9,12-dienoic acid + H2O | - |
Rhodococcus erythropolis | (12Z)-10-hydroxyoctadec-12-enoic acid | - |
? | |
| 4.2.1.53 | (9Z,12Z)-octadec-9,12-dienoic acid + H2O | - |
Rhodococcus erythropolis DN1 | (12Z)-10-hydroxyoctadec-12-enoic acid | - |
? | |
| 4.2.1.53 | (9Z,12Z,15Z)-octadec-9,12,15-trienoic acid + H2O | - |
Rhodococcus erythropolis | (12Z,15Z)-10-hydroxyoctadec-12,15-dienoic acid | - |
? | |
| 4.2.1.53 | (9Z,12Z,15Z)-octadec-9,12,15-trienoic acid + H2O | - |
Rhodococcus erythropolis DN1 | (12Z,15Z)-10-hydroxyoctadec-12,15-dienoic acid | - |
? | |
| 4.2.1.53 | additional information | neither detectable activity for any saturated fatty acid tested (C14:0 to C18:0), nor for myristoleic acid, vaccenic acid, eicosatrienoic acid and eicosatetraenoic acid | Rhodococcus erythropolis | ? | - |
? | |
| 4.2.1.53 | additional information | neither detectable activity for any saturated fatty acid tested (C14:0 to C18:0), nor for myristoleic acid, vaccenic acid, eicosatrienoic acid and eicosatetraenoic acid | Rhodococcus erythropolis DN1 | ? | - |
? | |
| 4.2.1.53 | oleate + H2O | - |
Rhodococcus erythropolis | 10-hydroxystearic acid | - |
? | |
| 4.2.1.53 | Palmitoleic acid + H2O | - |
Rhodococcus erythropolis | 10-Hydroxypalmitic acid | - |
? | |
| 4.2.1.53 | Palmitoleic acid + H2O | - |
Rhodococcus erythropolis DN1 | 10-Hydroxypalmitic acid | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.1.53 | monomer | 1 * 63825, calculated from sequence | Rhodococcus erythropolis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.53 | 28 | - |
- |
Rhodococcus erythropolis |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.53 | 20 | 45 | 20°C: about 72% of maximal activity, 45°C: about 21% of maximal activity | Rhodococcus erythropolis |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.53 | 34 | - |
oleate | pH 7.2, 28°C | Rhodococcus erythropolis | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.53 | 7.2 | - |
- |
Rhodococcus erythropolis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.53 | 5 | 8 | more than 85% of maximal activity in the range from pH 5.0 to 8.0 | Rhodococcus erythropolis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.53 | FAD | binds FAD | Rhodococcus erythropolis | |
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