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Literature summary extracted from

  • Deng, H.; McMahon, S.; Eustáquio, A.; Moore, B.; Naismith, J.; OHagan, D.
    Mechanistic insights into water activation in SAM hydroxide adenosyltransferase (duf-62) (2009), ChemBioChem, 10, 2455-2459 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.13.2.3 expressed in Escherichia coli BL21(DE3) cells Salinispora arenicola
3.13.2.3 expressed in Escherichia coli Rosetta cells Pyrococcus horikoshii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.13.2.3 sitting drop vapor diffusion method, using PEG 6000 (19.83 %), KCl (0.26 M) and MOPS (0.1 M, pH 7.0) Pyrococcus horikoshii
3.13.2.3 sitting drop vapor diffusion method, using PEG 6000 (19.83 %), KCl (0.26 M) and MOPS (0.1 M, pH 7.0) Salinispora arenicola

Protein Variants

EC Number Protein Variants Comment Organism
3.13.2.3 D72A inactive Salinispora arenicola
3.13.2.3 H130A the mutant shows about 25% of wild type activity Salinispora arenicola
3.13.2.3 H130Y the mutant shows about 5% of wild type activity Salinispora arenicola
3.13.2.3 R79A the mutant shows about 0.15% of wild type activity Salinispora arenicola

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.13.2.3 S-adenosyl-L-homocysteine
-
 Pyrococcus horikoshii
3.13.2.3 S-adenosyl-L-homocysteine
-
 Salinispora arenicola

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.13.2.3 0.001
-
 S-adenosyl-L-methionine wild type enzyme, at pH 7.9 and 37°C Salinispora arenicola
3.13.2.3 0.0014
-
 S-adenosyl-L-methionine mutant enzyme H130A, at pH 7.9 and 37°C Salinispora arenicola
3.13.2.3 0.0017
-
 S-adenosyl-L-methionine mutant enzyme H130Y, at pH 7.9 and 37°C Salinispora arenicola

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.13.2.3 S-adenosyl-L-methionine + H2O Pyrococcus horikoshii
-
 adenosine + L-methionine
-
 ?
3.13.2.3 S-adenosyl-L-methionine + H2O Salinispora arenicola
-
 adenosine + L-methionine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.13.2.3 Pyrococcus horikoshii O58212
-
-
3.13.2.3 Salinispora arenicola
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.13.2.3
-
 Pyrococcus horikoshii
3.13.2.3
-
 Salinispora arenicola

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.13.2.3 S-adenosyl-L-methionine + H2O
-
 Pyrococcus horikoshii adenosine + L-methionine
-
 ?
3.13.2.3 S-adenosyl-L-methionine + H2O
-
 Salinispora arenicola adenosine + L-methionine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.13.2.3 duf-62
-
 Pyrococcus horikoshii
3.13.2.3 duf-62
-
 Salinispora arenicola
3.13.2.3 S-adenosyl-L-methionine hydrolase
-
 Pyrococcus horikoshii
3.13.2.3 S-adenosyl-L-methionine hydrolase
-
 Salinispora arenicola
3.13.2.3 SAM hydroxide adenosyltransferase
-
 Pyrococcus horikoshii
3.13.2.3 SAM hydroxide adenosyltransferase
-
 Salinispora arenicola

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.13.2.3 0.00066
-
 S-adenosyl-L-methionine mutant enzyme H130Y, at pH 7.9 and 37°C Salinispora arenicola
3.13.2.3 0.0027
-
 S-adenosyl-L-methionine mutant enzyme H130A, at pH 7.9 and 37°C Salinispora arenicola
3.13.2.3 0.0075
-
 S-adenosyl-L-methionine wild type enzyme, at pH 7.9 and 37°C Salinispora arenicola

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.13.2.3 0.38
-
 S-adenosyl-L-methionine mutant enzyme H130Y, at pH 7.9 and 37°C Salinispora arenicola
3.13.2.3 1.9
-
 S-adenosyl-L-methionine mutant enzyme H130A, at pH 7.9 and 37°C Salinispora arenicola
3.13.2.3 7.5
-
 S-adenosyl-L-methionine wild type enzyme, at pH 7.9 and 37°C Salinispora arenicola