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Literature summary extracted from

  • Li, Y.; Sun, X.; Feng, Y.; Yuan, Q.
    Cloning, expression and activity optimization of trehalose synthase from Thermus thermophilus HB27 (2017), Chem. Eng. Sci., 135, 323-329 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.4.99.16 Ca2+ recombinant enzyme by 19% Thermus thermophilus
5.4.99.16 DTT recombinant enzyme by 41% Thermus thermophilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.16 recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta (DE3). Plasmids with different promoters and copy numbers are important for the expression of trehalose synthase genes in Escherichia coli. The trehalose synthase from Thermus thermophilus is best expressed from plasmid pET-22b showing high activity in trehalose production with 1.996 U/mg protein, co-expression of molecular chaperones sigma32, GroEL, GroES, DnaK and DnaJ greatly increasing the solubility of the trehalose synthase protein Thermus thermophilus

Protein Variants

EC Number Protein Variants Comment Organism
5.4.99.16 additional information enzyme expression in Escherichia coli strain Rosetta (DE3) for trehalose production, method optimization to 50°C, pH 9.0, 10 h, 10% maltose solution, and co-expression of molecular chaperones sigma32, GroEL, GroES, DnaK and DnaJ greatly increasing the solubility of the trehalose synthase protein. Addition of Ca2+ and DTT increase the activity by 19% and 41%, respectively Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.99.16 Mg2+ about 50% inhibition Thermus thermophilus
5.4.99.16 Mn2+ 85% inhibition Thermus thermophilus
5.4.99.16 Zn2+ 80% inhibition Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.99.16 maltose Thermus thermophilus
-
alpha,alpha-trehalose
-
r

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.16 Thermus thermophilus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.16 maltose
-
Thermus thermophilus alpha,alpha-trehalose
-
r

Synonyms

EC Number Synonyms Comment Organism
5.4.99.16 Trehalose synthase
-
Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.4.99.16 50
-
recombinant enzyme Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.99.16 9
-
recombinant enzyme Thermus thermophilus