EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
5.4.99.16 | Ca2+ | recombinant enzyme by 19% | Thermus thermophilus | |
5.4.99.16 | DTT | recombinant enzyme by 41% | Thermus thermophilus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
5.4.99.16 | recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta (DE3). Plasmids with different promoters and copy numbers are important for the expression of trehalose synthase genes in Escherichia coli. The trehalose synthase from Thermus thermophilus is best expressed from plasmid pET-22b showing high activity in trehalose production with 1.996 U/mg protein, co-expression of molecular chaperones sigma32, GroEL, GroES, DnaK and DnaJ greatly increasing the solubility of the trehalose synthase protein | Thermus thermophilus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.4.99.16 | additional information | enzyme expression in Escherichia coli strain Rosetta (DE3) for trehalose production, method optimization to 50°C, pH 9.0, 10 h, 10% maltose solution, and co-expression of molecular chaperones sigma32, GroEL, GroES, DnaK and DnaJ greatly increasing the solubility of the trehalose synthase protein. Addition of Ca2+ and DTT increase the activity by 19% and 41%, respectively | Thermus thermophilus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.4.99.16 | Mg2+ | about 50% inhibition | Thermus thermophilus | |
5.4.99.16 | Mn2+ | 85% inhibition | Thermus thermophilus | |
5.4.99.16 | Zn2+ | 80% inhibition | Thermus thermophilus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.99.16 | maltose | Thermus thermophilus | - |
alpha,alpha-trehalose | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.4.99.16 | Thermus thermophilus | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.99.16 | maltose | - |
Thermus thermophilus | alpha,alpha-trehalose | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.4.99.16 | Trehalose synthase | - |
Thermus thermophilus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.4.99.16 | 50 | - |
recombinant enzyme | Thermus thermophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.4.99.16 | 9 | - |
recombinant enzyme | Thermus thermophilus |