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Literature summary extracted from

  • Kim, S.; Sung, B.H.; Kim, S.C.; Lee, H.S.
    Genetic incorporation of l-dihydroxyphenylalanine (DOPA) biosynthesized by a tyrosine phenol-lyase (2018), Chem. Commun. (Camb.), 54, 3002-3005 .
    View publication on PubMed

Application

EC Number Application Comment Organism
4.1.99.2 synthesis the tyrosine phenol lyase (TPL) catalyzes synthesis of L-DOPA and can be used for large scale L-DOPA synthesis with direct incorporation of L-DOPA into proteins Citrobacter freundii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.99.2 gene tnaA, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH10beta Citrobacter freundii

Protein Variants

EC Number Protein Variants Comment Organism
4.1.99.2 additional information L-dihydroxyphenylalanine (DOPA) is biosynthesized by a tyrosine-phenol lyase from catechol, pyruvate, and ammonia in recombinant Escherichia coli expressing the enzyme, and the biosynthesized amino acid is directly incorporated into proteins. Three biochemical experiments with mutant proteins containing DOPA confirm the genetic incorporation of biosynthesized DOPA, and reveal its potential for various biochemical applications Citrobacter freundii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.99.2 L-tyrosine + H2O Citrobacter freundii
-
 phenol + pyruvate + NH3
-
 r
4.1.99.2 L-tyrosine + H2O Citrobacter freundii ATCC 8090
-
 phenol + pyruvate + NH3
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.2 Citrobacter freundii P31013
-
-
4.1.99.2 Citrobacter freundii ATCC 8090 P31013
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.99.2 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Citrobacter freundii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.2 catechol + pyruvate
-
 Citrobacter freundii L-DOPA + H2O
-
 r
4.1.99.2 catechol + pyruvate
-
 Citrobacter freundii ATCC 8090 L-DOPA + H2O
-
 r
4.1.99.2 L-tyrosine + H2O
-
 Citrobacter freundii phenol + pyruvate + NH3
-
 r
4.1.99.2 L-tyrosine + H2O
-
 Citrobacter freundii ATCC 8090 phenol + pyruvate + NH3
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.1.99.2 TPL
-
 Citrobacter freundii

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.99.2 pyridoxal 5'-phosphate dependent on Citrobacter freundii