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Literature summary extracted from

  • Bodea, S.; Funk, M.A.; Balskus, E.P.; Drennan, C.L.
    Molecular basis of C-N bond cleavage by the glycyl radical enzyme choline trimethylamine-lyase (2016), Cell Chem. Biol., 23, 1206-1216 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.3.99.4 optimized crystals are grown using hanging drop vapor diffusion at 21°C Oleidesulfovibrio alaskensis

Protein Variants

EC Number Protein Variants Comment Organism
4.3.99.4 E491A no activity detectable Oleidesulfovibrio alaskensis
4.3.99.4 E491Q no activity detectable Oleidesulfovibrio alaskensis
4.3.99.4 T502A turnover number for choline is 4400fold lower than for wild-type enzyme Oleidesulfovibrio alaskensis
4.3.99.4 Y208F turnover number for choline is 215fold lower than for wild-type enzyme Oleidesulfovibrio alaskensis
4.3.99.4 Y208F/Y506F turnover number for choline is 1050fold lower than for wild-type enzyme Oleidesulfovibrio alaskensis
4.3.99.4 Y506F turnover number for choline is 48fold lower than for wild-type enzyme Oleidesulfovibrio alaskensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.99.4 0.12
-
 choline pH 8.0, 21°C, mutant enzyme Y506F Oleidesulfovibrio alaskensis
4.3.99.4 0.13
-
 choline pH 8.0, 21°C, wild-type enzyme Oleidesulfovibrio alaskensis
4.3.99.4 0.22
-
 choline pH 8.0, 21°C, mutant enzyme Y208F Oleidesulfovibrio alaskensis
4.3.99.4 1.53
-
 choline pH 8.0, 21°C, mutant enzyme Y208F/Y506F Oleidesulfovibrio alaskensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.99.4 choline Oleidesulfovibrio alaskensis important route for the production of trimethylamine trimethylamine + acetaldehyde
-
 ?
4.3.99.4 choline Oleidesulfovibrio alaskensis G20 important route for the production of trimethylamine trimethylamine + acetaldehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.99.4 Oleidesulfovibrio alaskensis Q30W70
-
-
4.3.99.4 Oleidesulfovibrio alaskensis G20 Q30W70
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.99.4 choline
-
 Oleidesulfovibrio alaskensis trimethylamine + acetaldehyde
-
 ?
4.3.99.4 choline important route for the production of trimethylamine Oleidesulfovibrio alaskensis trimethylamine + acetaldehyde
-
 ?
4.3.99.4 choline
-
 Oleidesulfovibrio alaskensis G20 trimethylamine + acetaldehyde
-
 ?
4.3.99.4 choline important route for the production of trimethylamine Oleidesulfovibrio alaskensis G20 trimethylamine + acetaldehyde
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.3.99.4 cutC
-
 Oleidesulfovibrio alaskensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3.99.4 0.036
-
 choline pH 8.0, 21°C, mutant enzyme T502A Oleidesulfovibrio alaskensis
4.3.99.4 0.15
-
 choline pH 8.0, 21°C, mutant enzyme Y208F/Y506F Oleidesulfovibrio alaskensis
4.3.99.4 0.73
-
 choline pH 8.0, 21°C, mutant enzyme Y208F Oleidesulfovibrio alaskensis
4.3.99.4 3.27
-
 choline pH 8.0, 21°C, mutant enzyme Y506F Oleidesulfovibrio alaskensis
4.3.99.4 157
-
 choline pH 8.0, 21°C, wild-type enzyme Oleidesulfovibrio alaskensis