Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Andrews, F.; Wechsler, C.; Rogers, M.; Meyer, D.; Tittmann, K.; McLeish, M.
    Mechanistic and structural insight to an evolved benzoylformate decarboxylase with enhanced pyruvate decarboxylase activity (2016), Catalysts, 6, 190 .
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.7 mutant enzyme T377L/A460Y, hanging drop vapor diffusion method, using 0.1 M Tris (pH 8.5), 0.15 M CaCl2 and 22% PEG 400 (w/w) Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.7 T377L/A460Y the mutant exhibits a greater than 10000fold increase in pyruvate/benzoylformate substrate utilization ratio compared to that of the wild type enzyme Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.7 0.14
-
4-methyl-2-oxopentanoate mutant enzyme T377L/A460Y, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 0.14
-
2-Oxohexanoate mutant enzyme T377L/A460Y, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 0.14
-
4-methylthio-2-oxobutanoate mutant enzyme T377L/A460Y, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 0.15
-
2-oxopentanoate mutant enzyme T377L/A460Y, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 0.27
-
benzoylformate wild type enzyme, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 0.39
-
2-oxobutanoate mutant enzyme T377L/A460Y, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 0.45
-
benzoylformate mutant enzyme T377L/A460Y, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 0.77
-
3-methyl-2-oxobutanoate mutant enzyme T377L/A460Y, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 0.82
-
phenylpyruvate mutant enzyme T377L/A460Y, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 1.2
-
3-methyl-2-oxopentanoate mutant enzyme T377L/A460Y, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 1.3
-
4-methylthio-2-oxobutanoate wild type enzyme, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 4.1
-
2-oxobutanoate wild type enzyme, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 5.3
-
2-Oxohexanoate wild type enzyme, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 9.6
-
4-methyl-2-oxopentanoate wild type enzyme, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 11
-
2-oxopentanoate wild type enzyme, at pH 6.0 and 30°C Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.7 Mg2+ required, 1 mM used in assay conditions Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.7 benzoylformate Pseudomonas putida
-
benzaldehyde + CO2
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.7 Pseudomonas putida P20906
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.7
-
Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.7 2-oxobutanoate
-
Pseudomonas putida propionaldehyde + CO2
-
?
4.1.1.7 2-oxohexanoate
-
Pseudomonas putida valeraldehyde + CO2
-
?
4.1.1.7 2-oxopentanoate
-
Pseudomonas putida butyraldehyde + CO2
-
?
4.1.1.7 3-methyl-2-oxobutanoate
-
Pseudomonas putida 2-methylpropanal + CO2
-
?
4.1.1.7 3-methyl-2-oxopentanoate
-
Pseudomonas putida 2-methylbutanal + CO2
-
?
4.1.1.7 4-methyl-2-oxopentanoate
-
Pseudomonas putida 3-methylbutanal + CO2
-
?
4.1.1.7 4-methylthio-2-oxobutanoate
-
Pseudomonas putida 3-(methylsulfanyl)propanal + CO2
-
?
4.1.1.7 benzoylformate
-
Pseudomonas putida benzaldehyde + CO2
-
?
4.1.1.7 benzoylformate highest activity Pseudomonas putida benzaldehyde + CO2
-
?
4.1.1.7 additional information the enzyme exhibits very limited activity with pyruvate Pseudomonas putida ?
-
?
4.1.1.7 Phenylpyruvate
-
Pseudomonas putida Phenylacetaldehyde + CO2
-
?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.7 BFDC
-
Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.7 1.1
-
2-oxopentanoate mutant enzyme T377L/A460Y, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 2
-
phenylpyruvate mutant enzyme T377L/A460Y, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 2.7
-
4-methyl-2-oxopentanoate mutant enzyme T377L/A460Y, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 4.1
-
2-Oxohexanoate wild type enzyme, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 4.5
-
4-methylthio-2-oxobutanoate wild type enzyme, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 4.6
-
4-methylthio-2-oxobutanoate mutant enzyme T377L/A460Y, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 5.9
-
2-Oxohexanoate mutant enzyme T377L/A460Y, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 6
-
2-oxobutanoate mutant enzyme T377L/A460Y, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 6
-
2-oxopentanoate wild type enzyme, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 7.5
-
2-oxobutanoate wild type enzyme, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 11
-
4-methyl-2-oxopentanoate wild type enzyme, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 12
-
3-methyl-2-oxobutanoate mutant enzyme T377L/A460Y, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 15
-
benzoylformate mutant enzyme T377L/A460Y, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 16
-
3-methyl-2-oxopentanoate mutant enzyme T377L/A460Y, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 320
-
benzoylformate wild type enzyme, at pH 6.0 and 30°C Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.7 thiamine diphosphate
-
Pseudomonas putida

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.1.7 0.1
-
phenylpyruvate wild type enzyme, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 0.5
-
2-oxobutanoate wild type enzyme, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 1.1
-
4-methyl-2-oxopentanoate wild type enzyme, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 1.3
-
2-Oxohexanoate wild type enzyme, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 1.6
-
3-methyl-2-oxopentanoate wild type enzyme, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 1.8
-
2-oxopentanoate wild type enzyme, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 2.5
-
phenylpyruvate mutant enzyme T377L/A460Y, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 2.6
-
3-methyl-2-oxobutanoate wild type enzyme, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 3.5
-
4-methylthio-2-oxobutanoate wild type enzyme, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 7.5
-
2-oxopentanoate mutant enzyme T377L/A460Y, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 13
-
3-methyl-2-oxopentanoate mutant enzyme T377L/A460Y, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 15
-
3-methyl-2-oxobutanoate mutant enzyme T377L/A460Y, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 16
-
2-oxobutanoate mutant enzyme T377L/A460Y, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 20
-
4-methyl-2-oxopentanoate mutant enzyme T377L/A460Y, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 33
-
4-methylthio-2-oxobutanoate mutant enzyme T377L/A460Y, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 34
-
benzoylformate mutant enzyme T377L/A460Y, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 42
-
2-Oxohexanoate mutant enzyme T377L/A460Y, at pH 6.0 and 30°C Pseudomonas putida
4.1.1.7 1180
-
benzoylformate wild type enzyme, at pH 6.0 and 30°C Pseudomonas putida