EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.1.1.7 | mutant enzyme T377L/A460Y, hanging drop vapor diffusion method, using 0.1 M Tris (pH 8.5), 0.15 M CaCl2 and 22% PEG 400 (w/w) | Pseudomonas putida |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.1.1.7 | T377L/A460Y | the mutant exhibits a greater than 10000fold increase in pyruvate/benzoylformate substrate utilization ratio compared to that of the wild type enzyme | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.7 | 0.14 | - |
4-methyl-2-oxopentanoate | mutant enzyme T377L/A460Y, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 0.14 | - |
2-Oxohexanoate | mutant enzyme T377L/A460Y, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 0.14 | - |
4-methylthio-2-oxobutanoate | mutant enzyme T377L/A460Y, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 0.15 | - |
2-oxopentanoate | mutant enzyme T377L/A460Y, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 0.27 | - |
benzoylformate | wild type enzyme, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 0.39 | - |
2-oxobutanoate | mutant enzyme T377L/A460Y, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 0.45 | - |
benzoylformate | mutant enzyme T377L/A460Y, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 0.77 | - |
3-methyl-2-oxobutanoate | mutant enzyme T377L/A460Y, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 0.82 | - |
phenylpyruvate | mutant enzyme T377L/A460Y, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 1.2 | - |
3-methyl-2-oxopentanoate | mutant enzyme T377L/A460Y, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 1.3 | - |
4-methylthio-2-oxobutanoate | wild type enzyme, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 4.1 | - |
2-oxobutanoate | wild type enzyme, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 5.3 | - |
2-Oxohexanoate | wild type enzyme, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 9.6 | - |
4-methyl-2-oxopentanoate | wild type enzyme, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 11 | - |
2-oxopentanoate | wild type enzyme, at pH 6.0 and 30°C | Pseudomonas putida |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.7 | Mg2+ | required, 1 mM used in assay conditions | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.7 | benzoylformate | Pseudomonas putida | - |
benzaldehyde + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.7 | Pseudomonas putida | P20906 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.1.7 | - |
Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.7 | 2-oxobutanoate | - |
Pseudomonas putida | propionaldehyde + CO2 | - |
? | |
4.1.1.7 | 2-oxohexanoate | - |
Pseudomonas putida | valeraldehyde + CO2 | - |
? | |
4.1.1.7 | 2-oxopentanoate | - |
Pseudomonas putida | butyraldehyde + CO2 | - |
? | |
4.1.1.7 | 3-methyl-2-oxobutanoate | - |
Pseudomonas putida | 2-methylpropanal + CO2 | - |
? | |
4.1.1.7 | 3-methyl-2-oxopentanoate | - |
Pseudomonas putida | 2-methylbutanal + CO2 | - |
? | |
4.1.1.7 | 4-methyl-2-oxopentanoate | - |
Pseudomonas putida | 3-methylbutanal + CO2 | - |
? | |
4.1.1.7 | 4-methylthio-2-oxobutanoate | - |
Pseudomonas putida | 3-(methylsulfanyl)propanal + CO2 | - |
? | |
4.1.1.7 | benzoylformate | - |
Pseudomonas putida | benzaldehyde + CO2 | - |
? | |
4.1.1.7 | benzoylformate | highest activity | Pseudomonas putida | benzaldehyde + CO2 | - |
? | |
4.1.1.7 | additional information | the enzyme exhibits very limited activity with pyruvate | Pseudomonas putida | ? | - |
? | |
4.1.1.7 | Phenylpyruvate | - |
Pseudomonas putida | Phenylacetaldehyde + CO2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.1.7 | BFDC | - |
Pseudomonas putida |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.7 | 1.1 | - |
2-oxopentanoate | mutant enzyme T377L/A460Y, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 2 | - |
phenylpyruvate | mutant enzyme T377L/A460Y, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 2.7 | - |
4-methyl-2-oxopentanoate | mutant enzyme T377L/A460Y, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 4.1 | - |
2-Oxohexanoate | wild type enzyme, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 4.5 | - |
4-methylthio-2-oxobutanoate | wild type enzyme, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 4.6 | - |
4-methylthio-2-oxobutanoate | mutant enzyme T377L/A460Y, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 5.9 | - |
2-Oxohexanoate | mutant enzyme T377L/A460Y, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 6 | - |
2-oxobutanoate | mutant enzyme T377L/A460Y, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 6 | - |
2-oxopentanoate | wild type enzyme, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 7.5 | - |
2-oxobutanoate | wild type enzyme, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 11 | - |
4-methyl-2-oxopentanoate | wild type enzyme, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 12 | - |
3-methyl-2-oxobutanoate | mutant enzyme T377L/A460Y, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 15 | - |
benzoylformate | mutant enzyme T377L/A460Y, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 16 | - |
3-methyl-2-oxopentanoate | mutant enzyme T377L/A460Y, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 320 | - |
benzoylformate | wild type enzyme, at pH 6.0 and 30°C | Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.7 | thiamine diphosphate | - |
Pseudomonas putida |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.7 | 0.1 | - |
phenylpyruvate | wild type enzyme, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 0.5 | - |
2-oxobutanoate | wild type enzyme, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 1.1 | - |
4-methyl-2-oxopentanoate | wild type enzyme, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 1.3 | - |
2-Oxohexanoate | wild type enzyme, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 1.6 | - |
3-methyl-2-oxopentanoate | wild type enzyme, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 1.8 | - |
2-oxopentanoate | wild type enzyme, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 2.5 | - |
phenylpyruvate | mutant enzyme T377L/A460Y, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 2.6 | - |
3-methyl-2-oxobutanoate | wild type enzyme, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 3.5 | - |
4-methylthio-2-oxobutanoate | wild type enzyme, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 7.5 | - |
2-oxopentanoate | mutant enzyme T377L/A460Y, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 13 | - |
3-methyl-2-oxopentanoate | mutant enzyme T377L/A460Y, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 15 | - |
3-methyl-2-oxobutanoate | mutant enzyme T377L/A460Y, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 16 | - |
2-oxobutanoate | mutant enzyme T377L/A460Y, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 20 | - |
4-methyl-2-oxopentanoate | mutant enzyme T377L/A460Y, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 33 | - |
4-methylthio-2-oxobutanoate | mutant enzyme T377L/A460Y, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 34 | - |
benzoylformate | mutant enzyme T377L/A460Y, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 42 | - |
2-Oxohexanoate | mutant enzyme T377L/A460Y, at pH 6.0 and 30°C | Pseudomonas putida | |
4.1.1.7 | 1180 | - |
benzoylformate | wild type enzyme, at pH 6.0 and 30°C | Pseudomonas putida |