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Literature summary extracted from

  • Yeom, S.J.; Kim, M.; Kim, S.K.; Lee, D.H.; Kwon, K.K.; Lee, H.; Kim, H.; Kim, D.M.; Lee, S.G.
    Molecular and biochemical characterization of a novel isoprene synthase from Metrosideros polymorpha (2018), BMC Plant Biol., 18, 118 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.27 expressed in Escherichia coli BL21(DE3) cells Metrosideros polymorpha

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.27 Ca2+ about 50% residual activity at 1 mM Metrosideros polymorpha
4.2.3.27 Co2+ about 75% residual activity at 1 mM Metrosideros polymorpha
4.2.3.27 Cu2+ about 85% residual activity at 1 mM Metrosideros polymorpha
4.2.3.27 EDTA complete inhibition at 1 mM Metrosideros polymorpha
4.2.3.27 Fe2+ about 5% residual activity at 1 mM Metrosideros polymorpha
4.2.3.27 Mg2+ about 55% residual activity at 1 mM Metrosideros polymorpha
4.2.3.27 Ni2+ about 80% residual activity at 1 mM Metrosideros polymorpha
4.2.3.27 Zn2+ about 90% residual activity at 1 mM Metrosideros polymorpha

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.27 8.11
-
 dimethylallyl diphosphate at pH 6.0 and 50°C Metrosideros polymorpha

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.27 Mn2+ required, maximum activity at 5 mM Mn2+ Metrosideros polymorpha

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.27 65000
-
 gel filtration Metrosideros polymorpha

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.27 dimethylallyl diphosphate Metrosideros polymorpha
-
 isoprene + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.27 Metrosideros polymorpha
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.27 IMAC column chromatography Metrosideros polymorpha

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.27 dimethylallyl diphosphate
-
 Metrosideros polymorpha isoprene + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.27 monomer 1 * 65000, SDS-PAGE Metrosideros polymorpha
4.2.3.27 monomer 1 * 64950, calculated from amino acid sequence Metrosideros polymorpha

Synonyms

EC Number Synonyms Comment Organism
4.2.3.27 ISPS
-
 Metrosideros polymorpha

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.27 55
-
-
 Metrosideros polymorpha

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.3.27 30 45 about 60% activity at 30°C, about 70% activity at 35°C, about 80% activity at 40°C, about 90% activity at 45°C and 55°C, about 85% activity at 60°C Metrosideros polymorpha

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.27 0.35
-
 dimethylallyl diphosphate at pH 6.0 and 50°C Metrosideros polymorpha

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.27 6
-
-
 Metrosideros polymorpha

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.3.27 5.5 7 at pH 5.5 and 7.0, the activity is approximately 40% of the maximum Metrosideros polymorpha

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.3.27 0.043
-
 dimethylallyl diphosphate at pH 6.0 and 50°C Metrosideros polymorpha