EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.90 | gene LRA3, genetic organization, L-rhamnose catabolism genes RHA1, LRA2, LRA3 and LRA4 are organized in a chromosomal gene cluster in Scheffersomyces stipitis. In Scheffersomyces stipitis, another transcription factor than TRC1 is found in the L-rhamnose cluster, FST14. This transcription factor is more up-regulated than TRC1 on L-rhamnose and it might be co-regulating the pectinolytic genes together with TRC1 | Scheffersomyces stipitis |
4.2.1.90 | gene lraC, genetic organization, genes lraA, lraB and lraC, as well as the L-rhamnose regulator RhaR are organized in a cluster | Aspergillus niger |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.1.90 | additional information | construction of a DELTAlraC knockout mutant from strain CBS 141257, gene deletion and transcriptomic analysis is performed to elucidate the function of LraC, protoplast-mediated transformations of Aspergillus niger. Deletion of genes lraA, lraB or lraC causes a reduction in expression level of the other genes of the L-rhamnose pathway. Strongly reduced expression levels of lraA, lraB, rhaR, rglB, rgxA and rgaeA are observed in the DELTAlraC mutant upon the 2 h transfer to L-rhamnonate compared to the reference strain. But none of the deletions of genes lraA, lraB or lraC results in accumulation of the inducer of the pathway regulator (RhaR) | Aspergillus niger |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.90 | L-rhamnonate | Aspergillus niger | - |
2-dehydro-3-deoxy-L-rhamnonate + H2O | - |
? | |
4.2.1.90 | L-rhamnonate | Scheffersomyces stipitis | - |
2-dehydro-3-deoxy-L-rhamnonate + H2O | - |
? | |
4.2.1.90 | L-rhamnonate | Scheffersomyces stipitis ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545 | - |
2-dehydro-3-deoxy-L-rhamnonate + H2O | - |
? | |
4.2.1.90 | L-rhamnonate | Aspergillus niger CBS 141257 | - |
2-dehydro-3-deoxy-L-rhamnonate + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.90 | Aspergillus niger | - |
the CBS 141257 strain is obtained by transformation of strain N593.20 with uridine (pyrG) from Aspergillus oryzae | - |
4.2.1.90 | Aspergillus niger CBS 141257 | - |
the CBS 141257 strain is obtained by transformation of strain N593.20 with uridine (pyrG) from Aspergillus oryzae | - |
4.2.1.90 | Scheffersomyces stipitis | A3LZU6 | i.e. Pichia stipitis | - |
4.2.1.90 | Scheffersomyces stipitis ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545 | A3LZU6 | i.e. Pichia stipitis | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
4.2.1.90 | mycelium | - |
Aspergillus niger | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.90 | L-rhamnonate | - |
Aspergillus niger | 2-dehydro-3-deoxy-L-rhamnonate + H2O | - |
? | |
4.2.1.90 | L-rhamnonate | - |
Scheffersomyces stipitis | 2-dehydro-3-deoxy-L-rhamnonate + H2O | - |
? | |
4.2.1.90 | L-rhamnonate | - |
Scheffersomyces stipitis ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545 | 2-dehydro-3-deoxy-L-rhamnonate + H2O | - |
? | |
4.2.1.90 | L-rhamnonate | - |
Aspergillus niger CBS 141257 | 2-dehydro-3-deoxy-L-rhamnonate + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.90 | LRA3 | - |
Scheffersomyces stipitis |
4.2.1.90 | LraC | - |
Aspergillus niger |
4.2.1.90 | PICST_50672 | - |
Scheffersomyces stipitis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.2.1.90 | malfunction | a double deletion of lraC and lraA in Aspergillus niger results in a strain that exhibits no L-rhamnose dehydrogenase activity and cannot consume or grow on L-rhamnose. Deletion of the clustered genes lraA, lraB or lraC causes a reduction in expression level of the other genes of the L-rhamnose pathway, rhtA is not expressed in the DELTAlraC and DELATrhaR mutants. Strongly reduced expression levels of lraA, lraB, rhaR, rglB, rgxA and rgaeA are observed in the DELTAlraC mutant upon the 2 h transfer to L-rhamnonate compared to the reference strain | Aspergillus niger |
4.2.1.90 | metabolism | L-rhamnonate dehydratase (LRA3) converts L-rhamnonate to 2-dehydro-3-deoxy-L-rhamnonate (L-KDR), catalyzing the third step of the non-phosphorylative pathway in Schefferomyces stipitis. The inducer of RhaR is beyond L-rhamnonate dehydratase (LraC) and is likely to be the 2-keto-3-L-deoxyrhamnonate | Scheffersomyces stipitis |
4.2.1.90 | metabolism | L-rhamnonate dehydratase (LraC) probably converts L-rhamnonate to 2-dehydro-3-deoxy-L-rhamnonate (L-KDR), catalyzing the third step of the non-phosphorylative pathway. The inducer of RhaR is beyond L-rhamnonate dehydratase (LraC) and is likely to be the 2-keto-3-L-deoxyrhamnonate | Aspergillus niger |