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Literature summary extracted from

  • Wang, Y.; Yang, C.; Xue, W.; Zhang, T.; Liu, X.; Ju, J.; Zhao, B.; Liu, D.
    Selection and characterization of alanine racemase inhibitors against Aeromonas hydrophila (2017), BMC Microbiol., 17, 122-138 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.1.1 gene alr-2, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Aeromonas hydrophila subsp. hydrophila

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.1.1 2-(9-acridinylamino)benzenepropanoic acid
-
 Aeromonas hydrophila subsp. hydrophila
5.1.1.1 anabellamide
-
 Aeromonas hydrophila subsp. hydrophila
5.1.1.1 D-cycloserine
-
 Aeromonas hydrophila subsp. hydrophila
5.1.1.1 hematoxylin
-
 Aeromonas hydrophila subsp. hydrophila
5.1.1.1 higenamine
-
 Aeromonas hydrophila subsp. hydrophila
5.1.1.1 homogentisic acid competitive inhibition, has minimal cytotoxicity against mammalian cells. Homogentisic acid binds to the active site of the racemase Aeromonas hydrophila subsp. hydrophila
5.1.1.1 hydroquinone noncompetitive inhibition, has minimal cytotoxicity against mammalian cells. Hydroquinone binds near the active center of alanine racemase Aeromonas hydrophila subsp. hydrophila
5.1.1.1 additional information inhibitor screening of a library of 2100 compounds, and molecular docking study Aeromonas hydrophila subsp. hydrophila
5.1.1.1 patulin
-
 Aeromonas hydrophila subsp. hydrophila
5.1.1.1 propyl gallate
-
 Aeromonas hydrophila subsp. hydrophila
5.1.1.1 quercetin
-
 Aeromonas hydrophila subsp. hydrophila
5.1.1.1 [4-(5-butyl-5-methyl-2(5H)-furanylidene)dihydro-3,5-dioxo-2(3H)-furanylidene]acetic acid
-
 Aeromonas hydrophila subsp. hydrophila

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.1.1 L-alanine Aeromonas hydrophila subsp. hydrophila
-
 D-alanine
-
 r
5.1.1.1 L-alanine Aeromonas hydrophila subsp. hydrophila HBNUAh01 / ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240
-
 D-alanine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.1 Aeromonas hydrophila subsp. hydrophila A0KL98
-
-
5.1.1.1 Aeromonas hydrophila subsp. hydrophila HBNUAh01 / ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240 A0KL98
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.1 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration Aeromonas hydrophila subsp. hydrophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.1 L-alanine
-
 Aeromonas hydrophila subsp. hydrophila D-alanine
-
 r
5.1.1.1 L-alanine
-
 Aeromonas hydrophila subsp. hydrophila HBNUAh01 / ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240 D-alanine
-
 r
5.1.1.1 additional information assay method optimization and validation, overview Aeromonas hydrophila subsp. hydrophila ?
-
 ?
5.1.1.1 additional information assay method optimization and validation, overview Aeromonas hydrophila subsp. hydrophila HBNUAh01 / ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.1.1.1 homodimer
-
 Aeromonas hydrophila subsp. hydrophila

Synonyms

EC Number Synonyms Comment Organism
5.1.1.1 alr-2
-
 Aeromonas hydrophila subsp. hydrophila
5.1.1.1 alr-2 gen ename Aeromonas hydrophila subsp. hydrophila

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.1.1 35
-
-
 Aeromonas hydrophila subsp. hydrophila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.1.1 11
-
-
 Aeromonas hydrophila subsp. hydrophila

Cofactor

EC Number Cofactor Comment Organism Structure
5.1.1.1 pyridoxal 5'-phosphate
-
 Aeromonas hydrophila subsp. hydrophila

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.1.1.1 0.0517
-
 homogentisic acid pH 11.0, 35°C, recombinant His-tagged enzyme Aeromonas hydrophila subsp. hydrophila
5.1.1.1 0.212
-
 hydroquinone pH 11.0, 35°C, recombinant His-tagged enzyme Aeromonas hydrophila subsp. hydrophila

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
5.1.1.1 0.0054
-
 pH 11.0, 35°C, recombinant His-tagged enzyme Aeromonas hydrophila subsp. hydrophila D-cycloserine
5.1.1.1 0.0066
-
 pH 11.0, 35°C, recombinant His-tagged enzyme Aeromonas hydrophila subsp. hydrophila anabellamide
5.1.1.1 0.0086
-
 pH 11.0, 35°C, recombinant His-tagged enzyme Aeromonas hydrophila subsp. hydrophila propyl gallate
5.1.1.1 0.0089
-
 pH 11.0, 35°C, recombinant His-tagged enzyme Aeromonas hydrophila subsp. hydrophila [4-(5-butyl-5-methyl-2(5H)-furanylidene)dihydro-3,5-dioxo-2(3H)-furanylidene]acetic acid
5.1.1.1 0.0125
-
 pH 11.0, 35°C, recombinant His-tagged enzyme Aeromonas hydrophila subsp. hydrophila homogentisic acid
5.1.1.1 0.0143
-
 pH 11.0, 35°C, recombinant His-tagged enzyme Aeromonas hydrophila subsp. hydrophila higenamine
5.1.1.1 0.0147
-
 pH 11.0, 35°C, recombinant His-tagged enzyme Aeromonas hydrophila subsp. hydrophila patulin
5.1.1.1 0.0155
-
 pH 11.0, 35°C, recombinant His-tagged enzyme Aeromonas hydrophila subsp. hydrophila quercetin
5.1.1.1 0.0156
-
 pH 11.0, 35°C, recombinant His-tagged enzyme Aeromonas hydrophila subsp. hydrophila hematoxylin
5.1.1.1 0.0177
-
 pH 11.0, 35°C, recombinant His-tagged enzyme Aeromonas hydrophila subsp. hydrophila 2-(9-acridinylamino)benzenepropanoic acid
5.1.1.1 0.0185
-
 pH 11.0, 35°C, recombinant His-tagged enzyme Aeromonas hydrophila subsp. hydrophila hydroquinone

General Information

EC Number General Information Comment Organism
5.1.1.1 additional information enzyme structure homology modeling using the crystal structure of Escherichia coli AlaR with PDB ID 2rjh Aeromonas hydrophila subsp. hydrophila
5.1.1.1 physiological function the enzyme catalyzes the interconversion of L-alanine to D-alanine. D-Alanine is an essential building block of the cell wall of both gram-positive and gram-negative bacteria Aeromonas hydrophila subsp. hydrophila