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Literature summary extracted from

  • Tsaballa, A.; Nikolaidis, A.; Trikka, F.; Ignea, C.; Kampranis, S.; Makris, A.; Argiriou, A.
    Use of the de novo transcriptome analysis of silver-leaf nightshade (Solanum elaeagnifolium) to identify gene expression changes associated with wounding and terpene biosynthesis (2015), BMC Genomics, 16, 504 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.117 gene cl9841, the TPS gene identified, transcript cl9841, is a cluster of 9 sequences but only one is expressed in leaves and flowers. The protein shares 78% similarity with Solanum lycopersicum TPS3 protein, a monoterpene (-)-camphene/tricyclene synthase (UniProt ID G1JUH1) and a putative (-)-camphene/tricyclene synthase from Solanum tuberosum, DNA and amino acid sequence determination and analysis, recombinant coexpression with copalyl diphosphate synthase from Salvia pomifera and Saccharomyces cerevisiae ERG20 mutant F96C producing GGPP in Saccharomyces cerevisiae strain AM 238 Solanum elaeagnifolium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.117 geranyl diphosphate Solanum elaeagnifolium
-
 (-)-camphene + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.117 Solanum elaeagnifolium
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.117 geranyl diphosphate
-
 Solanum elaeagnifolium (-)-camphene + diphosphate
-
 ?
4.2.3.117 additional information gene cl9841 is an active monoterpene synthase enzyme producing a range of monoterpenes with the most prominent being camphene (52.55%), beta-myrcene (11.01%), and limonene (10.44%) and several minor additional compounds. GC-MS qualitative and semi-quantitative analysis of terpene compounds, overview Solanum elaeagnifolium ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.117 cl9841
-
 Solanum elaeagnifolium

General Information

EC Number General Information Comment Organism
4.2.3.117 evolution the enzyme shares 78% similarity with Solanum lycopersicum TPS3 protein, a monoterpene (-)-camphene/tricyclene synthase (UniProt ID G1JUH1) and a putative (-)-camphene/tricyclene synthase from Solanum tuberosum, suggesting it to have (-)-camphene synthase activity Solanum elaeagnifolium