Any feedback?
Literature summary extracted from
- Characterization of an L-arabinose isomerase from Bacillus coagulans NL01 and its application for D-tagatose production (2016), BMC Biotechnol., 16, 55 .
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 5.3.1.4 | synthesis | under optimal conditions, recombinant Escherichia coli cells expressing AraA can convert 150 g/l and 250 g/l D-galactose to D-tagatose with conversion rates of 32% (32 h) and 27% (48 h) | Weizmannia coagulans |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.3.1.4 | expression in Escherichia coli | Weizmannia coagulans |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.1.4 | EDTA | 60% loss of activity | Weizmannia coagulans |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.1.4 | 269.8 | - |
L-arabinose | pH 7.5, 60°C | Weizmannia coagulans | |
| 5.3.1.4 | 355.1 | - |
D-galactose | pH 7.5, 60°C | Weizmannia coagulans | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.1.4 | Co2+ | 0.5 mM, 190% of the activity of EDTA-treated enzyme | Weizmannia coagulans | |
| 5.3.1.4 | Mn2+ | 0.5 mM, 270% of the activity of EDTA-treated enzyme | Weizmannia coagulans | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.1.4 | Weizmannia coagulans | A0A191W0D9 | - |
- |
| 5.3.1.4 | Weizmannia coagulans NL01 | A0A191W0D9 | - |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.4 | 1.1 | - |
substrate D-galactose, pH 7.5, 60°C | Weizmannia coagulans |
| 5.3.1.4 | 8 | - |
substrate L-arabinose, pH 7.5, 60°C | Weizmannia coagulans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.1.4 | D-galactose | - |
Weizmannia coagulans | D-tagatose | - |
? | |
| 5.3.1.4 | D-galactose | - |
Weizmannia coagulans NL01 | D-tagatose | - |
? | |
| 5.3.1.4 | L-arabinose | - |
Weizmannia coagulans | L-ribulose | - |
? | |
| 5.3.1.4 | L-arabinose | - |
Weizmannia coagulans NL01 | L-ribulose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.3.1.4 | ? | x * 55000, SDS-PAGE, x * 53500, calculated from sequence | Weizmannia coagulans |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.3.1.4 | AraA | - |
Weizmannia coagulans |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.4 | 60 | - |
- |
Weizmannia coagulans |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.4 | 50 | 70 | more than 85% of maximum activity | Weizmannia coagulans |
| 5.3.1.4 | 80 | - |
above 60% of maximum activity | Weizmannia coagulans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.4 | 7.5 | - |
- |
Weizmannia coagulans |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.4 | 8 | 9 | more than 90% of maximum activity | Weizmannia coagulans |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.1.4 | 0.017 | - |
D-galactose | pH 7.5, 60°C | Weizmannia coagulans | |
| 5.3.1.4 | 0.145 | - |
L-arabinose | pH 7.5, 60°C | Weizmannia coagulans | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
