EC Number | Application | Comment | Organism |
---|---|---|---|
4.2.1.123 | diagnostics | tetrahymanol is a polycyclic triterpenoid lipid in the Tetrahymena ciliates. The diagenetic product, gammacerane, is often used as a biomarker for water column stratification in ancient ecosystems | Trepomonas sp. PC1 |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.123 | gene TPC1_17222, sequence comparisons and phylogenetic analysis, transcriptome analysis | Trepomonas sp. PC1 |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.123 | squalene + H2O | Trepomonas sp. PC1 | - |
tetrahymanol | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.123 | Trepomonas sp. PC1 | A0A146K6G2 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.123 | squalene + H2O | - |
Trepomonas sp. PC1 | tetrahymanol | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.123 | squalene-tetrahymanol cyclase | - |
Trepomonas sp. PC1 |
4.2.1.123 | STC | - |
Trepomonas sp. PC1 |
4.2.1.123 | TPC1_17222 | - |
Trepomonas sp. PC1 |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.2.1.123 | evolution | phylogenetic evidence reveals that the last common ancestor of diplomonads was dependent on a host and that Trepomonas has adapted secondarily to a free-living lifestyle. Free-living taxa are nested within parasites reveals more examples of secondarily free-living eukaryotes, transcriptome study of Trepomonas sp. PC1, a diplomonad isolated from marine sediment. Phylogenetic analyses show that most of the differences in metabolic capacity between free-living Trepomonas and the parasitic diplomonads are due to recent acquisitions of bacterial genes via gene transfer. The transcriptome includes a gene encoding squalene-tetrahymanol cyclase. This enzyme synthesizes the sterol substitute tetrahymanol in the absence of oxygen, potentially allowing Trepomonas to thrive under anaerobic conditions as a free-living bacterivore, without depending on sterols from other eukaryotes | Trepomonas sp. PC1 |
4.2.1.123 | physiological function | the enzyme synthesizes the sterol substitute tetrahymanol in the absence of oxygen, potentially allowing Trepomonas to thrive under anaerobic conditions as a free-living bacterivore, without depending on sterols from other eukaryotes. Several microbial eukaryotes that live under anoxic conditions employ the sterol substitute tetrahymanol, which can be synthesized without molecular oxygen. The Trepomonas transcriptome includes a homologue of STC, the enzyme required to synthesize tetrahymanol | Trepomonas sp. PC1 |