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Literature summary extracted from

  • Ma, X.; Guo, J.; Ma, Y.; Jin, B.; Zhan, Z.; Yuan, Y.; Huang, L.
    Characterization of a monoterpene synthase from Paeonia lactiflora producing alpha-pinene as its single product (2016), Biotechnol. Lett., 38, 1213-1219 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.119 enzyme PlPIN, cloned from roots, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression in Escherichia coli strain Transetta (DE3) Paeonia lactiflora
4.2.3.121 enzyme PlPIN, cloned from roots, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression in Escherichia coli strain Transetta (DE3) Paeonia lactiflora

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.119 Mg2+ required Paeonia lactiflora
4.2.3.121 Mg2+ required Paeonia lactiflora

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.119 geranyl diphosphate Paeonia lactiflora
-
 alpha-pinene + diphosphate
-
 ?
4.2.3.121 geranyl diphosphate Paeonia lactiflora
-
 alpha-pinene + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.119 Paeonia lactiflora A0A0G3BIB7 i.e. Paeonia albiflora
-
4.2.3.121 Paeonia lactiflora A0A0G3BIB7 i.e. Paeonia albiflora
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.119 geranyl diphosphate
-
 Paeonia lactiflora alpha-pinene + diphosphate
-
 ?
4.2.3.119 geranyl diphosphate stereochemistry of the product is not specified in the publication Paeonia lactiflora alpha-pinene + diphosphate
-
 ?
4.2.3.119 additional information GC-MS analysis reaction product analysis. Enzyme PlPIN employs geranyl diphosphate as a specific substrate to produce a-pinene as a unique product Paeonia lactiflora ?
-
 ?
4.2.3.121 geranyl diphosphate
-
 Paeonia lactiflora alpha-pinene + diphosphate
-
 ?
4.2.3.121 additional information GC-MS analysis reaction product analysis. Enzyme PlPIN employs geranyl diphosphate as a specific substrate to produce alpha-pinene as a unique product Paeonia lactiflora ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.119 alpha pinene sythase
-
 Paeonia lactiflora
4.2.3.119 alpha-pinene sythase
-
 Paeonia lactiflora
4.2.3.119 More cf. EC 4.2.3.121 Paeonia lactiflora
4.2.3.119 PlPIN
-
 Paeonia lactiflora
4.2.3.121 alpha pinene sythase
-
 Paeonia lactiflora
4.2.3.121 alpha-pinene sythase
-
 Paeonia lactiflora
4.2.3.121 More cf. EC 4.2.3.119 Paeonia lactiflora
4.2.3.121 PlPIN
-
 Paeonia lactiflora

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.119 30
-
 assay at Paeonia lactiflora
4.2.3.121 30
-
 assay at Paeonia lactiflora

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.119 7
-
 assay at Paeonia lactiflora
4.2.3.121 7
-
 assay at Paeonia lactiflora

General Information

EC Number General Information Comment Organism
4.2.3.119 evolution the enzyme belongs to the TPS enzyme superfamily, clade TPS-b, where it is clustered with monoterpene synthases Paeonia lactiflora
4.2.3.119 physiological function the terpene synthase PlPIN from Paeonia lactiflora catalyzes the conversion of geranyl pyrophosphate to alpha-pinene and is involved in the biosynthesis of paeoniflorin Paeonia lactiflora
4.2.3.121 evolution the enzyme belongs to the TPS enzyme superfamily, clade TPS-b, where it is clustered with monoterpene synthases Paeonia lactiflora
4.2.3.121 physiological function the terpene synthase PlPIN from Paeonia lactiflora catalyzes the conversion of geranyl pyrophosphate to alpha-pinene and is involved in the biosynthesis of paeoniflorin Paeonia lactiflora