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Literature summary extracted from

  • Park, C.; Kim, J.; Lee, S.; Kim, Y.; Kang, L.; Oh, D.
    Characterization of a recombinant mannobiose 2-epimerase from Spirochaeta thermophila that is suggested to be a cellobiose 2-epimerase (2013), Biotechnol. Lett., 35, 1873-1880 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.11 expression in Escherichia coli ER2566 Spirochaeta thermophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.3.11 3.5
-
mannobiose pH 7.0, 60°C Spirochaeta thermophila
5.1.3.11 4.3
-
cellobiose pH 7.0, 60°C Spirochaeta thermophila

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.3.11 47000
-
SDS-PAGE, gel filtration Spirochaeta thermophila

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.11 Caldicellulosiruptor saccharolyticus A4XGA6
-
-
5.1.3.11 Spirochaeta thermophila E0RU15
-
-
5.1.3.11 Spirochaeta thermophila DSM 6724 E0RU15
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.11
-
Caldicellulosiruptor saccharolyticus
5.1.3.11
-
Spirochaeta thermophila

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.3.11 0.009
-
substrate: 4-O-alpha-D-glucopyranosyl-D-mannose, pH 7.0, 60°C Spirochaeta thermophila
5.1.3.11 0.02
-
substrate: D-glucose, pH 7.0, 60°C Spirochaeta thermophila
5.1.3.11 0.05
-
substrate: D-mannose, pH 7.0, 60°C Spirochaeta thermophila
5.1.3.11 0.1
-
substrate: cellotriose, pH 7.0, 60°C Spirochaeta thermophila
5.1.3.11 0.42
-
substrate: maltotriose, pH 7.0, 60°C Spirochaeta thermophila
5.1.3.11 6.3
-
substrate: mannotriose, pH 7.0, 60°C Spirochaeta thermophila
5.1.3.11 7.8
-
substrate: epilactose, pH 7.0, 60°C Spirochaeta thermophila
5.1.3.11 9.9
-
substrate: maltose, pH 7.0, 60°C Spirochaeta thermophila
5.1.3.11 14.3
-
substrate: 4-O-beta-D-glucopyranosyl-D-mannose, pH 7.0, 60°C Spirochaeta thermophila
5.1.3.11 15.5
-
substrate: lactose, pH 7.0, 60°C Spirochaeta thermophila
5.1.3.11 21.8
-
substrate: cellobiose, pH 7.0, 60°C Caldicellulosiruptor saccharolyticus
5.1.3.11 25.5
-
substrate: mannobiose, pH 7.0, 60°C Spirochaeta thermophila
5.1.3.11 29.2
-
substrate: cellobiose, pH 7.0, 60°C Spirochaeta thermophila
5.1.3.11 41.9
-
substrate: mannobiose, pH 7.0, 60°C Caldicellulosiruptor saccharolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.11 4-O-alpha-D-glucopyranosyl-D-mannose
-
Spirochaeta thermophila maltose
-
r
5.1.3.11 4-O-beta-D-glucopyranosyl-D-mannose
-
Spirochaeta thermophila cellobiose
-
r
5.1.3.11 4-O-beta-D-mannopyranosyl-D-glucose
-
Spirochaeta thermophila mannobiose
-
r
5.1.3.11 cellobiose i.e. 4-O-beta-D-glucopyranosyl-D-mannose Caldicellulosiruptor saccharolyticus 4-O-beta-D-glucopyranosyl-D-mannose
-
r
5.1.3.11 cellobiose i.e. 4-O-beta-D-glucopyranosyl-D-mannose Spirochaeta thermophila 4-O-beta-D-glucopyranosyl-D-mannose
-
r
5.1.3.11 cellobiose i.e. 4-O-beta-D-glucopyranosyl-D-mannose Spirochaeta thermophila DSM 6724 4-O-beta-D-glucopyranosyl-D-mannose
-
r
5.1.3.11 cellotriose
-
Spirochaeta thermophila 4-O-beta-D-glucopyranosyl-O-beta-D-glucopyranosyl-D-mannose
-
r
5.1.3.11 D-glucose
-
Spirochaeta thermophila D-mannose
-
r
5.1.3.11 D-glucose
-
Spirochaeta thermophila DSM 6724 D-mannose
-
r
5.1.3.11 D-mannose
-
Spirochaeta thermophila D-glucose
-
r
5.1.3.11 D-mannose
-
Spirochaeta thermophila DSM 6724 D-glucose
-
r
5.1.3.11 epilactose i.e. 4-O-beta-D-galactopyranosyl-D-mannose Spirochaeta thermophila lactose
-
r
5.1.3.11 lactose i.e. 4-O-beta-D-galactopyranosyl-D-glucose Spirochaeta thermophila epilactose
-
r
5.1.3.11 lactose i.e. 4-O-beta-D-galactopyranosyl-D-glucose Spirochaeta thermophila DSM 6724 epilactose
-
r
5.1.3.11 maltose i.e.4-O-alpha-D-glucopyranosyl-D-glucose Spirochaeta thermophila 4-O-alpha-D-glucopyranosyl-D-mannose
-
r
5.1.3.11 maltotriose
-
Spirochaeta thermophila 4-O-alpha-D-glucopyranosyl-O-alpha-D-glucopyranosyl-D-mannose
-
r
5.1.3.11 mannobiose i.e 4-O-b-D-mannopyranosyl-D-mannose Caldicellulosiruptor saccharolyticus 4-O-beta-D-mannopyranosyl-D-glucose
-
r
5.1.3.11 mannobiose i.e 4-O-beta-D-mannopyranosyl-D-mannose Spirochaeta thermophila 4-O-beta-D-mannopyranosyl-D-glucose
-
r
5.1.3.11 mannotriose
-
Spirochaeta thermophila 4-O-beta-D-mannopyranosyl-O-beta-D-mannopyranosyl-D-glucose
-
r
5.1.3.11 additional information the enzyme exhibits a higher epimerization activity for mannose or the mannose moiety at the reducing end of beta- and alpha-1,4-glycosylmannose than for glucose or the glucose moiety of beta- and alpha-1,4-glycosyl-glucose Spirochaeta thermophila ?
-
?
5.1.3.11 additional information the enzyme exhibits a higher epimerization activity for mannose or the mannose moiety at the reducing end of beta- and alpha-1,4-glycosylmannose than for glucose or the glucose moiety of beta- and alpha-1,4-glycosyl-glucose Spirochaeta thermophila DSM 6724 ?
-
?

Subunits

EC Number Subunits Comment Organism
5.1.3.11 monomer 1 * 47000, SDS-PAGE Spirochaeta thermophila

Synonyms

EC Number Synonyms Comment Organism
5.1.3.11 mannobiose 2-epimerase
-
Spirochaeta thermophila

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.3.11 60
-
-
Spirochaeta thermophila

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.1.3.11 50 70 50°C: about 80% of maximal activity, 70°C: about 60% of maximal activity Spirochaeta thermophila

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.1.3.11 55
-
half-life: 717 h Spirochaeta thermophila
5.1.3.11 60
-
half-life: 124 h Spirochaeta thermophila
5.1.3.11 65
-
half-life: 28 h Spirochaeta thermophila
5.1.3.11 70
-
half-life: 0.3 h Spirochaeta thermophila

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1.3.11 2 8 cellobiose pH 7.0, 60°C Spirochaeta thermophila
5.1.3.11 35.7
-
mannobiose pH 7.0, 60°C Spirochaeta thermophila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.3.11 7
-
-
Spirochaeta thermophila

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.1.3.11 6 8 pH 6.0: about 60% of maximal activity, pH 8.0: about 50% of maximal activity Spirochaeta thermophila

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.1.3.11 6.5
-
cellobiose pH 7.0, 60°C Spirochaeta thermophila
5.1.3.11 10.2
-
mannobiose pH 7.0, 60°C Spirochaeta thermophila