EC Number | Cloned (Comment) | Organism |
---|---|---|
5.1.3.11 | expression in Escherichia coli ER2566 | Spirochaeta thermophila |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.1.3.11 | 3.5 | - |
mannobiose | pH 7.0, 60°C | Spirochaeta thermophila | |
5.1.3.11 | 4.3 | - |
cellobiose | pH 7.0, 60°C | Spirochaeta thermophila |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.1.3.11 | 47000 | - |
SDS-PAGE, gel filtration | Spirochaeta thermophila |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.1.3.11 | Caldicellulosiruptor saccharolyticus | A4XGA6 | - |
- |
5.1.3.11 | Spirochaeta thermophila | E0RU15 | - |
- |
5.1.3.11 | Spirochaeta thermophila DSM 6724 | E0RU15 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.1.3.11 | - |
Caldicellulosiruptor saccharolyticus |
5.1.3.11 | - |
Spirochaeta thermophila |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
5.1.3.11 | 0.009 | - |
substrate: 4-O-alpha-D-glucopyranosyl-D-mannose, pH 7.0, 60°C | Spirochaeta thermophila |
5.1.3.11 | 0.02 | - |
substrate: D-glucose, pH 7.0, 60°C | Spirochaeta thermophila |
5.1.3.11 | 0.05 | - |
substrate: D-mannose, pH 7.0, 60°C | Spirochaeta thermophila |
5.1.3.11 | 0.1 | - |
substrate: cellotriose, pH 7.0, 60°C | Spirochaeta thermophila |
5.1.3.11 | 0.42 | - |
substrate: maltotriose, pH 7.0, 60°C | Spirochaeta thermophila |
5.1.3.11 | 6.3 | - |
substrate: mannotriose, pH 7.0, 60°C | Spirochaeta thermophila |
5.1.3.11 | 7.8 | - |
substrate: epilactose, pH 7.0, 60°C | Spirochaeta thermophila |
5.1.3.11 | 9.9 | - |
substrate: maltose, pH 7.0, 60°C | Spirochaeta thermophila |
5.1.3.11 | 14.3 | - |
substrate: 4-O-beta-D-glucopyranosyl-D-mannose, pH 7.0, 60°C | Spirochaeta thermophila |
5.1.3.11 | 15.5 | - |
substrate: lactose, pH 7.0, 60°C | Spirochaeta thermophila |
5.1.3.11 | 21.8 | - |
substrate: cellobiose, pH 7.0, 60°C | Caldicellulosiruptor saccharolyticus |
5.1.3.11 | 25.5 | - |
substrate: mannobiose, pH 7.0, 60°C | Spirochaeta thermophila |
5.1.3.11 | 29.2 | - |
substrate: cellobiose, pH 7.0, 60°C | Spirochaeta thermophila |
5.1.3.11 | 41.9 | - |
substrate: mannobiose, pH 7.0, 60°C | Caldicellulosiruptor saccharolyticus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.1.3.11 | 4-O-alpha-D-glucopyranosyl-D-mannose | - |
Spirochaeta thermophila | maltose | - |
r | |
5.1.3.11 | 4-O-beta-D-glucopyranosyl-D-mannose | - |
Spirochaeta thermophila | cellobiose | - |
r | |
5.1.3.11 | 4-O-beta-D-mannopyranosyl-D-glucose | - |
Spirochaeta thermophila | mannobiose | - |
r | |
5.1.3.11 | cellobiose | i.e. 4-O-beta-D-glucopyranosyl-D-mannose | Caldicellulosiruptor saccharolyticus | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
5.1.3.11 | cellobiose | i.e. 4-O-beta-D-glucopyranosyl-D-mannose | Spirochaeta thermophila | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
5.1.3.11 | cellobiose | i.e. 4-O-beta-D-glucopyranosyl-D-mannose | Spirochaeta thermophila DSM 6724 | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
5.1.3.11 | cellotriose | - |
Spirochaeta thermophila | 4-O-beta-D-glucopyranosyl-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
5.1.3.11 | D-glucose | - |
Spirochaeta thermophila | D-mannose | - |
r | |
5.1.3.11 | D-glucose | - |
Spirochaeta thermophila DSM 6724 | D-mannose | - |
r | |
5.1.3.11 | D-mannose | - |
Spirochaeta thermophila | D-glucose | - |
r | |
5.1.3.11 | D-mannose | - |
Spirochaeta thermophila DSM 6724 | D-glucose | - |
r | |
5.1.3.11 | epilactose | i.e. 4-O-beta-D-galactopyranosyl-D-mannose | Spirochaeta thermophila | lactose | - |
r | |
5.1.3.11 | lactose | i.e. 4-O-beta-D-galactopyranosyl-D-glucose | Spirochaeta thermophila | epilactose | - |
r | |
5.1.3.11 | lactose | i.e. 4-O-beta-D-galactopyranosyl-D-glucose | Spirochaeta thermophila DSM 6724 | epilactose | - |
r | |
5.1.3.11 | maltose | i.e.4-O-alpha-D-glucopyranosyl-D-glucose | Spirochaeta thermophila | 4-O-alpha-D-glucopyranosyl-D-mannose | - |
r | |
5.1.3.11 | maltotriose | - |
Spirochaeta thermophila | 4-O-alpha-D-glucopyranosyl-O-alpha-D-glucopyranosyl-D-mannose | - |
r | |
5.1.3.11 | mannobiose | i.e 4-O-b-D-mannopyranosyl-D-mannose | Caldicellulosiruptor saccharolyticus | 4-O-beta-D-mannopyranosyl-D-glucose | - |
r | |
5.1.3.11 | mannobiose | i.e 4-O-beta-D-mannopyranosyl-D-mannose | Spirochaeta thermophila | 4-O-beta-D-mannopyranosyl-D-glucose | - |
r | |
5.1.3.11 | mannotriose | - |
Spirochaeta thermophila | 4-O-beta-D-mannopyranosyl-O-beta-D-mannopyranosyl-D-glucose | - |
r | |
5.1.3.11 | additional information | the enzyme exhibits a higher epimerization activity for mannose or the mannose moiety at the reducing end of beta- and alpha-1,4-glycosylmannose than for glucose or the glucose moiety of beta- and alpha-1,4-glycosyl-glucose | Spirochaeta thermophila | ? | - |
? | |
5.1.3.11 | additional information | the enzyme exhibits a higher epimerization activity for mannose or the mannose moiety at the reducing end of beta- and alpha-1,4-glycosylmannose than for glucose or the glucose moiety of beta- and alpha-1,4-glycosyl-glucose | Spirochaeta thermophila DSM 6724 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.1.3.11 | monomer | 1 * 47000, SDS-PAGE | Spirochaeta thermophila |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.1.3.11 | mannobiose 2-epimerase | - |
Spirochaeta thermophila |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.1.3.11 | 60 | - |
- |
Spirochaeta thermophila |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.1.3.11 | 50 | 70 | 50°C: about 80% of maximal activity, 70°C: about 60% of maximal activity | Spirochaeta thermophila |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.1.3.11 | 55 | - |
half-life: 717 h | Spirochaeta thermophila |
5.1.3.11 | 60 | - |
half-life: 124 h | Spirochaeta thermophila |
5.1.3.11 | 65 | - |
half-life: 28 h | Spirochaeta thermophila |
5.1.3.11 | 70 | - |
half-life: 0.3 h | Spirochaeta thermophila |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.1.3.11 | 2 | 8 | cellobiose | pH 7.0, 60°C | Spirochaeta thermophila | |
5.1.3.11 | 35.7 | - |
mannobiose | pH 7.0, 60°C | Spirochaeta thermophila |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.1.3.11 | 7 | - |
- |
Spirochaeta thermophila |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
5.1.3.11 | 6 | 8 | pH 6.0: about 60% of maximal activity, pH 8.0: about 50% of maximal activity | Spirochaeta thermophila |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.1.3.11 | 6.5 | - |
cellobiose | pH 7.0, 60°C | Spirochaeta thermophila | |
5.1.3.11 | 10.2 | - |
mannobiose | pH 7.0, 60°C | Spirochaeta thermophila |