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Literature summary extracted from

  • Okano, K.; Uematsu, G.; Hama, S.; Tanaka, T.; Noda, H.; Kondo, A.; Honda, K.
    Metabolic engineering of Lactobacillus plantarum for direct L-lactic acid production from raw corn starch (2018), Biotechnol. J., 13, e1700517 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.2.1 gene larA encoded in the larA-E operon Lactiplantibacillus plantarum

Protein Variants

EC Number Protein Variants Comment Organism
5.1.2.1 additional information construction of an engineered Lactobacillus plantarum strain NCIMB 8826, which enables the production of optically pure L-lactate from raw starch for costeffective lactate production. Disruption of the operon encoding lactate racemase (larA-E), which catalyzes the interconversion between D- and L-lactate, completely abolishes D-lactate production. The DELTAldhD DELTAlarA-E mutant produces 87.0 g/l of L-lactate with an optical purity of 99.4%. A plasmid is introduced into the DELTAldhD DELTAlarA-E mutant for the secretion of alpha-amylase from Streptococcus bovis strain 148. The resulting strain produces 50.3 g/l of L-lactate from raw corn starch with a yield of 0.91 g per g of consumed sugar and an optical purity of 98.6% Lactiplantibacillus plantarum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.2.1 (S)-lactate Lactiplantibacillus plantarum
-
 (R)-lactate
-
 r
5.1.2.1 (S)-lactate Lactiplantibacillus plantarum ATCC BAA-793 / NCIMB 8826 / WCFS1
-
 (R)-lactate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.1.2.1 Lactiplantibacillus plantarum F9USS9
-
-
5.1.2.1 Lactiplantibacillus plantarum ATCC BAA-793 / NCIMB 8826 / WCFS1 F9USS9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.2.1 (S)-lactate
-
 Lactiplantibacillus plantarum (R)-lactate
-
 r
5.1.2.1 (S)-lactate
-
 Lactiplantibacillus plantarum ATCC BAA-793 / NCIMB 8826 / WCFS1 (R)-lactate
-
 r

Synonyms

EC Number Synonyms Comment Organism
5.1.2.1 LARa
-
 Lactiplantibacillus plantarum

General Information

EC Number General Information Comment Organism
5.1.2.1 malfunction disruption of the operon encoding lactate racemase (larA-E), which catalyzes the interconversion between D- and L-latate, completely abolishes D-lactate production. An engineered Lactobacillus plantarum strain lacking the enzyme is useful in the production of L-lactate from starchy materials Lactiplantibacillus plantarum