Any feedback?
Literature summary extracted from
- Identification and characterization of 3,6-anhydro-L-galactonate cycloisomerase belonging to theenolase superfamily (2015), Biotechnol. Bioprocess Eng., 20, 462-472 .
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.5.1.25 | expressed in Escherichia coli BL21 cells | Postechiella marina |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.5.1.25 | Ba2+ | about 50% residual activity at 1 mM | Postechiella marina |  |
| 5.5.1.25 | Ca2+ | about 15% residual activity at 1 mM | Postechiella marina | |
| 5.5.1.25 | Cd2+ | about 30% residual activity at 1 mM | Postechiella marina | |
| 5.5.1.25 | Cu2+ | about 40% residual activity at 1 mM | Postechiella marina | |
| 5.5.1.25 | EDTA | about 40% residual activity at 1 mM | Postechiella marina | |
| 5.5.1.25 | Hg2+ | about 45% residual activity at 1 mM | Postechiella marina | |
| 5.5.1.25 | Ni2+ | about 55% residual activity at 1 mM | Postechiella marina | |
| 5.5.1.25 | Zn2+ | about 20% residual activity at 1 mM | Postechiella marina | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.5.1.25 | Co2+ | about 118% activity at 1 mM | Postechiella marina | |
| 5.5.1.25 | Mg2+ | about 115% activity at 1 mM | Postechiella marina | |
| 5.5.1.25 | additional information | not influenced by Mn2+ and Fe2+ | Postechiella marina |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.5.1.25 | 3,6-anhydro-L-galactonate | Postechiella marina | - |
2-dehydro-3-deoxy-L-galactonate | - |
? | |
| 5.5.1.25 | 3,6-anhydro-L-galactonate | Postechiella marina M091 | - |
2-dehydro-3-deoxy-L-galactonate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.5.1.25 | Postechiella marina | A0A0F7C8X0 | - |
- |
| 5.5.1.25 | Postechiella marina M091 | A0A0F7C8X0 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.5.1.25 | Ni-NTA agarose column chromatography | Postechiella marina |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.5.1.25 | 3,6-anhydro-L-galactonate | - |
Postechiella marina | 2-dehydro-3-deoxy-L-galactonate | - |
? | |
| 5.5.1.25 | 3,6-anhydro-L-galactonate | 100% activity | Postechiella marina | 2-dehydro-3-deoxy-L-galactonate | - |
r | |
| 5.5.1.25 | 3,6-anhydro-L-galactonate | - |
Postechiella marina M091 | 2-dehydro-3-deoxy-L-galactonate | - |
? | |
| 5.5.1.25 | 3,6-anhydro-L-galactonate | 100% activity | Postechiella marina M091 | 2-dehydro-3-deoxy-L-galactonate | - |
r | |
| 5.5.1.25 | galactarate | 1.8% activity compared to 3,6-anhydro-L-galactonate | Postechiella marina | ? | - |
? | |
| 5.5.1.25 | galactarate | 1.8% activity compared to 3,6-anhydro-L-galactonate | Postechiella marina M091 | ? | - |
? | |
| 5.5.1.25 | additional information | no activity with D-gluconate, D-galactonate, D-glucuronate, D-glucarate, L-galactono-1,4-lactone, D-lactate, L-lactate, D,L-glycerate, D,L-tartrate, or L-malate | Postechiella marina | ? | - |
? | |
| 5.5.1.25 | additional information | no activity with D-gluconate, D-galactonate, D-glucuronate, D-glucarate, L-galactono-1,4-lactone, D-lactate, L-lactate, D,L-glycerate, D,L-tartrate, or L-malate | Postechiella marina M091 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.5.1.25 | ? | x * 43000, SDS-PAGE | Postechiella marina |
| 5.5.1.25 | ? | x * 40358, calculated from amino acid sequence | Postechiella marina |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.5.1.25 | L-AnGA cycloisomerase | - |
Postechiella marina |
| 5.5.1.25 | L-AnGACI | - |
Postechiella marina |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.5.1.25 | 30 | - |
- |
Postechiella marina |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.5.1.25 | 10 | 60 | about 30% activity at 10°C, about 75% activity at 20°C, 100% activity at 30°C, about 90% activity at 40°C, about 60% activity at 50°C, about 30% activity at 60°C | Postechiella marina |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.5.1.25 | 8 | - |
- |
Postechiella marina |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.5.1.25 | 5 | 10 | about 50% activity at pH 5.0, about 70% activity at pH 6.0, about 90% activity at pH 7.0, 100% activity at pH 8.0, about 70% activity at pH 9.0, about 40% activity at pH 10.0 | Postechiella marina |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
