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Literature summary extracted from

  • Park, C.
    Characterization of a recombinant L-rhamnose isomerase from Bacillus subtilis and its application on production of L-lyxose and L-mannose (2014), Biotechnol. Bioprocess Eng., 19, 18-25 .
No PubMed abstract available

Application

EC Number Application Comment Organism
5.3.1.14 synthesis 40 g/l L-lyxose is produced from 100 g/l L-xylulose by the enzyme during 60 min, while 25 g/l L-mannose is produced from 100 g/l L-fructose in 80 min Bacillus subtilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.14 expression in Escherichia coli Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.14 53
-
 L-rhamnose pH 8.0, 60°C Bacillus subtilis
5.3.1.14 86
-
 L-Lyxose pH 8.0, 60°C Bacillus subtilis
5.3.1.14 97
-
 L-Mannose pH 8.0, 60°C Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.14 Mn2+ 1 mM, 25fold increase in activity Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.14 194000
-
 gel filtration Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.14 Bacillus subtilis
-
-
-
5.3.1.14 Bacillus subtilis ATCC 23857
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.14 0.47
-
 substrate L-allose, pH 8.0, 60°C Bacillus subtilis
5.3.1.14 0.92
-
 substrate L-mannose, pH 8.0, 60°C Bacillus subtilis
5.3.1.14 2.58
-
 substrate L-lyxose, pH 8.0, 60°C Bacillus subtilis
5.3.1.14 3.58
-
 substrate L-rhamnose, pH 8.0, 60°C Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.14 D-allose
-
 Bacillus subtilis D-psicose
-
 r
5.3.1.14 D-allose
-
 Bacillus subtilis ATCC 23857 D-psicose
-
 r
5.3.1.14 D-gulose
-
 Bacillus subtilis D-sorbose
-
 r
5.3.1.14 D-gulose
-
 Bacillus subtilis ATCC 23857 D-sorbose
-
 r
5.3.1.14 L-lyxose
-
 Bacillus subtilis L-xylulose
-
 r
5.3.1.14 L-lyxose
-
 Bacillus subtilis ATCC 23857 L-xylulose
-
 r
5.3.1.14 L-Mannose
-
 Bacillus subtilis L-Fructose
-
 r
5.3.1.14 L-Mannose
-
 Bacillus subtilis ATCC 23857 L-Fructose
-
 r
5.3.1.14 L-Rhamnose
-
 Bacillus subtilis L-Rhamnulose
-
 r
5.3.1.14 L-Rhamnose
-
 Bacillus subtilis ATCC 23857 L-Rhamnulose
-
 r

Subunits

EC Number Subunits Comment Organism
5.3.1.14 tetramer 4 * 48600, SDS-PAGE, 4 * 49468, calculated from sequence Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
5.3.1.14 RhaA
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.14 60
-
-
 Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.14 70
-
 2 h, 53% residual activity Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.14 37
-
 L-Mannose pH 8.0, 60°C Bacillus subtilis
5.3.1.14 53
-
 L-Lyxose pH 8.0, 60°C Bacillus subtilis
5.3.1.14 153
-
 L-rhamnose pH 8.0, 60°C Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.14 8
-
-
 Bacillus subtilis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.3.1.14 0.38
-
 L-Mannose pH 8.0, 60°C Bacillus subtilis
5.3.1.14 0.62
-
 L-Lyxose pH 8.0, 60°C Bacillus subtilis
5.3.1.14 2.89
-
 L-rhamnose pH 8.0, 60°C Bacillus subtilis