BRENDA - Enzyme Database

Expression, purification, and biological characterization of Anaplasma phagocytophilum enolase

Gao, X.; Zheng, C.; Liu, X.; Biosci. Trends 11, 651-657 (2018)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.11
expression in Escherichia coli
Anaplasma phagocytophilum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
6.053
-
2-phospho-D-glycerate
pH 7.0, 30°C
Anaplasma phagocytophilum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Anaplasma phagocytophilum
Q2GK24
-
-
4.2.1.11
Anaplasma phagocytophilum HZ
Q2GK24
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
747330
Anaplasma phagocytophilum
phosphoenolpyruvate + H2O
-
-
-
?
4.2.1.11
2-phospho-D-glycerate
-
747330
Anaplasma phagocytophilum HZ
phosphoenolpyruvate + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 72000, SDS-PAGE, recombinant protein with GST-tag
Anaplasma phagocytophilum
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.1.11
50
-
-
Anaplasma phagocytophilum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
8
-
-
Anaplasma phagocytophilum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
expression in Escherichia coli
Anaplasma phagocytophilum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
6.053
-
2-phospho-D-glycerate
pH 7.0, 30°C
Anaplasma phagocytophilum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
747330
Anaplasma phagocytophilum
phosphoenolpyruvate + H2O
-
-
-
?
4.2.1.11
2-phospho-D-glycerate
-
747330
Anaplasma phagocytophilum HZ
phosphoenolpyruvate + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 72000, SDS-PAGE, recombinant protein with GST-tag
Anaplasma phagocytophilum
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.1.11
50
-
-
Anaplasma phagocytophilum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
8
-
-
Anaplasma phagocytophilum
General Information
EC Number
General Information
Commentary
Organism
4.2.1.11
physiological function
recombinant enolase retains its enzymatic activity and binds to human plasminogen. Binding can be significantly reduced in the presence of epsilon-aminocaproic acid. Eno promotes plasminogen to plasmin conversion in the presence of plasminogen activator
Anaplasma phagocytophilum
General Information (protein specific)
EC Number
General Information
Commentary
Organism
4.2.1.11
physiological function
recombinant enolase retains its enzymatic activity and binds to human plasminogen. Binding can be significantly reduced in the presence of epsilon-aminocaproic acid. Eno promotes plasminogen to plasmin conversion in the presence of plasminogen activator
Anaplasma phagocytophilum