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Literature summary extracted from

  • Kihara, T.; Hiroe, A.; Ishii-Hyakutake, M.; Mizuno, K.; Tsuge, T.
    Bacillus cereus-type polyhydroxyalkanoate biosynthetic gene cluster contains R-specific enoyl-CoA hydratase gene (2017), Biosci. Biotechnol. Biochem., 81, 1627-1635 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.119 gene phaJ_1, DNA and amino acid sequence determination and analysis, sequence comparisons, the gene encoding MaoC-like protein locates in the pha cluster, functional recombinant expression in Escherichia coli strains DH5alpha and LS5218 Bacillus cereus

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.119 additional information in an in vivo assay using Escherichia coli as a host for polyhydroxyalkanoate (PHA) accumulation, the recombinant strain expressing PhaJYB4 and PHA synthase leads to increased PHA accumulation, suggesting that PhaJYB4 functioned as a monomer supplier. The pha cluster from Bacillus cereus strain YB-4 functions to accumulate PHA in Escherichia coli, but it does not function when the phaJYB4 gene is deleted. The recombinant strain utilizes butyrate, valerate, hexanoate, and dodecanoate for PHA synthesis Bacillus cereus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.119 (2E)-2-enoyl-CoA + H2O Bacillus cereus
-
 (3R)-3-hydroxyacyl-CoA
-
 r
4.2.1.119 (2E)-2-enoyl-CoA + H2O Bacillus cereus YB-4
-
 (3R)-3-hydroxyacyl-CoA
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.119 Bacillus cereus A0A150CP14
-
-
4.2.1.119 Bacillus cereus YB-4 A0A150CP14
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.119 (2E)-2-enoyl-CoA + H2O
-
 Bacillus cereus (3R)-3-hydroxyacyl-CoA
-
 r
4.2.1.119 (2E)-2-enoyl-CoA + H2O
-
 Bacillus cereus YB-4 (3R)-3-hydroxyacyl-CoA
-
 r
4.2.1.119 (2E)-crotonyl-CoA + H2O
-
 Bacillus cereus (3R)-3-hydroxybutanoyl-CoA
-
 r
4.2.1.119 (2E)-crotonyl-CoA + H2O
-
 Bacillus cereus YB-4 (3R)-3-hydroxybutanoyl-CoA
-
 r
4.2.1.119 additional information PhaJYB4 activity is thought to be specific for short chain-length enoyl-CoA Bacillus cereus ?
-
 ?
4.2.1.119 additional information PhaJYB4 activity is thought to be specific for short chain-length enoyl-CoA Bacillus cereus YB-4 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.119 MaoC-like protein
-
 Bacillus cereus
4.2.1.119 phaJ
-
 Bacillus cereus
4.2.1.119 PhaJYB4
-
 Bacillus cereus
4.2.1.119 R-hydratase
-
 Bacillus cereus
4.2.1.119 R-specific enoyl-CoA hydratase
-
 Bacillus cereus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.119 30
-
 assay at Bacillus cereus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.119 8
-
 assay at Bacillus cereus

General Information

EC Number General Information Comment Organism
4.2.1.119 evolution enzyme PhaJYB4 shows a high homology to short chain-length (C4-C6)-specific PhaJs, e.g. Pseudomonas aeruginosa PhaJ1Pa and Aeromonas caviae PhaJAc, rather than medium chain-length (C8-C12)-specific PhaJs, e.g. Pseudomonas aeruginosa PhaJ4Pa4 Bacillus cereus
4.2.1.119 physiological function Bacillus cereus accumulates polyhydroxyalkanoate (PHA). The MaoC-like protein has an R-specific enoyl-CoA hydratase activity and is referred to as PhaJ when involved in the PHA metabolism. In an in vivo assay using Escherichia coli as a host for PHA accumulation, the recombinant strain expressing PhaJYB4 and PHA synthase leads to increased PHA accumulation, suggesting that PhaJYB4 functioned as a monomer supplier Bacillus cereus