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Literature summary extracted from

  • Raghava, S.; Gupta, M.
    Purification and characterization of an alcohol dehydrogenase with an unusual specificity towards glycerol from Thermus thermophilus (2010), Biores. Technol., 101, 2554-2557 .
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.6 0.005
-
 NADH pH and temperature not specified in the publication Thermus thermophilus
1.1.1.6 0.015
-
 NAD+ pH and temperature not specified in the publication Thermus thermophilus
1.1.1.6 0.07
-
 D-glyceraldehyde pH and temperature not specified in the publication Thermus thermophilus
1.1.1.6 0.2
-
 glycerol pH and temperature not specified in the publication Thermus thermophilus
1.1.1.6 0.3
-
 2-butanol pH and temperature not specified in the publication Thermus thermophilus
1.1.1.6 3
-
 1-phenylethanol pH and temperature not specified in the publication Thermus thermophilus
1.1.1.6 4
-
 2-propanol pH and temperature not specified in the publication Thermus thermophilus
1.1.1.6 35
-
 ethanol pH and temperature not specified in the publication Thermus thermophilus
1.1.1.6 46
-
 1-propanol pH and temperature not specified in the publication Thermus thermophilus
1.1.1.6 56
-
 ethylene glycol pH and temperature not specified in the publication Thermus thermophilus
1.1.1.6 71
-
 1,4-Butanediol pH and temperature not specified in the publication Thermus thermophilus
1.1.1.6 76
-
 benzyl alcohol pH and temperature not specified in the publication Thermus thermophilus
1.1.1.6 840
-
 1-butanol pH and temperature not specified in the publication Thermus thermophilus
1.1.1.6 840
-
 1-Octanol pH and temperature not specified in the publication Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.6 Zn2+ contains Zn2+ Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.6 43000
-
 SDS-PAGE Thermus thermophilus
1.1.1.6 170000
-
 analytical ultracentrifugation Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.6 Thermus thermophilus
-
-
-
1.1.1.6 Thermus thermophilus MTCC 1494
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.6 purified with 25fold purification and 68% yield using a single chromatographic step Thermus thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.6 18.5
-
 pH and temperature not specified in the publication Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.6 1,4-butanediol + NAD+
-
 Thermus thermophilus ? + NADH + H+
-
 ?
1.1.1.6 1-butanol + NAD+
-
 Thermus thermophilus butanal + NADH + H+
-
 ?
1.1.1.6 1-butanol + NAD+
-
 Thermus thermophilus MTCC 1494 butanal + NADH + H+
-
 ?
1.1.1.6 1-octanol + NAD+
-
 Thermus thermophilus octanal + NADH + H+
-
 ?
1.1.1.6 1-octanol + NAD+
-
 Thermus thermophilus MTCC 1494 octanal + NADH + H+
-
 ?
1.1.1.6 1-phenylethanol + NAD+
-
 Thermus thermophilus acetophenone + NADH + H+
-
 ?
1.1.1.6 1-propanol + NAD+
-
 Thermus thermophilus propanal + NADH + H+
-
 ?
1.1.1.6 1-propanol + NAD+
-
 Thermus thermophilus MTCC 1494 propanal + NADH + H+
-
 ?
1.1.1.6 2-butanol + NAD+
-
 Thermus thermophilus 2-butanone + NADH + H+
-
 ?
1.1.1.6 2-propanol + NAD+
-
 Thermus thermophilus acetone + NADH + H+
-
 ?
1.1.1.6 2-propanol + NAD+
-
 Thermus thermophilus MTCC 1494 acetone + NADH + H+
-
 ?
1.1.1.6 benzyl alcohol + NAD+
-
 Thermus thermophilus benzaldehyde + NADH + H+
-
 ?
1.1.1.6 D-glyceraldehyde + NADH + H+
-
 Thermus thermophilus glycerol + NAD+
-
 r
1.1.1.6 ethanol + NAD+
-
 Thermus thermophilus acetaldehyde + NADH + H+
-
 ?
1.1.1.6 ethylene glycol + NAD+
-
 Thermus thermophilus ? + NADH + H+
-
 ?
1.1.1.6 glycerol + NAD+ no activity with NADP+. The enzyme shows much higher activity towards glycerol as compared to short chain primary and secondary alcohols. The thermostable enzyme is highly stereospecific in oxidation of glycerol and converts glycerol into D-glyceraldehyde Thermus thermophilus D-glyceraldehyde + NADH + H+
-
 r
1.1.1.6 glycerol + NAD+ no activity with NADP+. The enzyme shows much higher activity towards glycerol as compared to short chain primary and secondary alcohols. The thermostable enzyme is highly stereospecific in oxidation of glycerol and converts glycerol into D-glyceraldehyde Thermus thermophilus MTCC 1494 D-glyceraldehyde + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.6 homotetramer 4 * 43000, SDS-PAGE Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.6 TTHADH
-
 Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.6 80
-
 glycerol oxidation Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.6 89
-
 Tm-value Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.6 8.8
-
 glycerol oxidation Thermus thermophilus