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Literature summary extracted from

  • Yu, J.; Liu, Q.; Liu, L.; Chen, J.
    Cloning and characterization of dichloromethane dehalogenase from Methylobacterium rhodesianum for dichloromethane degradation (2017), Bioremediat. J., 21, 71-80 .
No PubMed abstract available

Application

EC Number Application Comment Organism
4.5.1.3 degradation enzyme activity in recombinant cells is 3 times higher than that in the wild-type Methylorubrum rhodesianum. Degradation efficiency of dichloromethane reaches 86.11% within 20 h and is highly associated with glutathione concentration Methylorubrum rhodesianum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.5.1.3 expression in Escherichia coli Methylorubrum rhodesianum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.5.1.3 10.96
-
 Dichloromethane pH 7.0, 40°C Methylorubrum rhodesianum

Organism

EC Number Organism UniProt Comment Textmining
4.5.1.3 Methylorubrum rhodesianum A0A088MAR6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.5.1.3 recombinant protein Methylorubrum rhodesianum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.5.1.3 dichloromethane + H2O
-
 Methylorubrum rhodesianum formaldehyde + 2 Cl-
-
 ?

Subunits

EC Number Subunits Comment Organism
4.5.1.3 ? x * 33058, calculated from sequence, x * 33000, SDS-PAGE Methylorubrum rhodesianum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.5.1.3 40
-
-
 Methylorubrum rhodesianum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5.1.3 7 8
-
 Methylorubrum rhodesianum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.5.1.3 Methylorubrum rhodesianum calculated from sequence
-
 5.1