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Literature summary extracted from

  • Jun, S.Y.; Kim, S.H.; Kanth, B.K.; Lee, J.; Pack, S.P.
    Expression and characterization of a codon-optimized alkaline-stable carbonic anhydrase from Aliivibrio salmonicida for CO2 sequestration applications (2017), Bioprocess Biosyst. Eng., 40, 413-421 .
    View publication on PubMed

Application

EC Number Application Comment Organism
4.2.1.1 environmental protection recombinant engineered mASCA enzyme exhibits high production yield and sufficient stabilities against relatively high temperature and alkaline pH, which are required conditions for the development of more efficient enzymatic CCS systems. Carbon capture and storage (CCS) is a technology that can capture up to 90% of the carbon dioxide (CO2) emissions produced from the use of fossil fuels in electricity generation and industrial processes, preventing the carbon dioxide from entering the atmosphere. mASCA has the potential to play an important role in CCS systems, particularly in an enzyme-based CO2 capture system that requires large amounts of CA enzyme Aliivibrio salmonicida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.1 recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Aliivibrio salmonicida

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.1 additional information design of a mature form ofASCA(mASCA) using a codon optimization of ASCA gene and removal of the ASCA signal peptide. mASCA is highly expressed and highly stable. The recombinant engineered enzyme ASCA can promote CO2 absorption in an alkaline solvent required for efficient carbon capture Aliivibrio salmonicida

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.1 acetazolamide about 70% inhibition at 1 mM Aliivibrio salmonicida
4.2.1.1 azide about 30% inhibition at 1 mM Aliivibrio salmonicida
4.2.1.1 Iodide about 30% inhibition at 1 mM Aliivibrio salmonicida
4.2.1.1 nitrate about 70% inhibition at 1 mM Aliivibrio salmonicida
4.2.1.1 sulfanilamide about 70% inhibition at 1 mM Aliivibrio salmonicida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.1 Zn2+ required Aliivibrio salmonicida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.1 H2CO3 Aliivibrio salmonicida
-
CO2 + H2O
-
r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.1 Aliivibrio salmonicida B6EIU7 i.e. Vibrio salmonicida strain LFI1238
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.1 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis Aliivibrio salmonicida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.1 H2CO3
-
Aliivibrio salmonicida CO2 + H2O
-
r
4.2.1.1 additional information recombinant engineered enzyme ASCA exhibits esterase activity with 4-nitrophenyl acetate and can promote CO2 absorption in an alkaline solvent required for efficient carbon capture. Recombinant mASCA is capable of catalyzing the conversion of CO2 to CaCO3 (calcite form) in the presence of Ca2+. The CO2 hydration activity is determined by monitoring the time required for the pH of the assay solution to change from 8.3 to 7.0 and using the formula [[(t0 - tc)/tc]/protein(mg)] [Wilbur-Anderson units (WAU) per mg of protein] Aliivibrio salmonicida ?
-
?

Subunits

EC Number Subunits Comment Organism
4.2.1.1 ? x * 26000, about, recombinant optimized enzyme without signal peptide, SDS-PAGE, x * 25715, sequence calculation Aliivibrio salmonicida

Synonyms

EC Number Synonyms Comment Organism
4.2.1.1 alpha-type CA
-
Aliivibrio salmonicida
4.2.1.1 ASCA
-
Aliivibrio salmonicida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.1 22
-
CO2 hydration assay at room temperature Aliivibrio salmonicida
4.2.1.1 25
-
esterase activity assay at, p-NPA hydrolysis Aliivibrio salmonicida

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.1.1 10 60 activity range, esterase activity of recombinant enzyme Aliivibrio salmonicida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.1 65
-
purified recombinant enzyme mASCA, retains over 90% activity after 15 min Aliivibrio salmonicida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.1 8
-
-
Aliivibrio salmonicida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.1 6 11 activity range, esterase activity of recombinant enzyme Aliivibrio salmonicida

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.1.1 4
-
purified recombinant enzyme mASCA, about 30% activity remaining after 15 min Aliivibrio salmonicida
4.2.1.1 5
-
purified recombinant enzyme mASCA, about 70% activity remaining after 15 min Aliivibrio salmonicida
4.2.1.1 6 11 purified recombinant enzyme mASCA, over 90% activity remaining after 15 min within this range Aliivibrio salmonicida
4.2.1.1 10
-
purified recombinant enzyme mASCA, over 90% activity remaining after 48 h, 70% after 72 h Aliivibrio salmonicida