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Literature summary extracted from

  • De Luca, V.; Vullo, D.; Del Prete, S.; Carginale, V.; Osman, S.M.; AlOthman, Z.; Supuran, C.T.; Capasso, C.
    Cloning, characterization and anion inhibition studies of a gamma-carbonic anhydrase from the Antarctic bacterium Colwellia psychrerythraea (2016), Bioorg. Med. Chem., 24, 835-840 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.1 gene CpsCAgamma, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) (Arctic Express DE3 competent cells), subcloning in Escherichia coli strain DH5alpha Colwellia psychrerythraea

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.1 acetazolamide sulfonamide CA inhibitor (CAI) Colwellia psychrerythraea
4.2.1.1 B4O72-
-
 Colwellia psychrerythraea
4.2.1.1 CF3SO3-
-
 Colwellia psychrerythraea
4.2.1.1 Cl-
-
 Colwellia psychrerythraea
4.2.1.1 CN-
-
 Colwellia psychrerythraea
4.2.1.1 CNO-
-
 Colwellia psychrerythraea
4.2.1.1 CO32-
-
 Colwellia psychrerythraea
4.2.1.1 CS32-
-
 Colwellia psychrerythraea
4.2.1.1 Et2NCS2-
-
 Colwellia psychrerythraea
4.2.1.1 FSO3-
-
 Colwellia psychrerythraea
4.2.1.1 H2NSO2NH2
-
 Colwellia psychrerythraea
4.2.1.1 H2NSO3H
-
 Colwellia psychrerythraea
4.2.1.1 HCO3-
-
 Colwellia psychrerythraea
4.2.1.1 HS-
-
 Colwellia psychrerythraea
4.2.1.1 HSO3-
-
 Colwellia psychrerythraea
4.2.1.1 I-
-
 Colwellia psychrerythraea
4.2.1.1 additional information perchlorate, tetrafluoroborate, azide, fluoride, and bromide are not inhibitory for the enzyme Colwellia psychrerythraea
4.2.1.1 NH(SO3)22-
-
 Colwellia psychrerythraea
4.2.1.1 NO2-
-
 Colwellia psychrerythraea
4.2.1.1 NO3-
-
 Colwellia psychrerythraea
4.2.1.1 P2O74-
-
 Colwellia psychrerythraea
4.2.1.1 PF6-
-
 Colwellia psychrerythraea
4.2.1.1 Ph-AsO3H2
-
 Colwellia psychrerythraea
4.2.1.1 Ph-B(OH)2
-
 Colwellia psychrerythraea
4.2.1.1 ReO4-
-
 Colwellia psychrerythraea
4.2.1.1 RuO4-
-
 Colwellia psychrerythraea
4.2.1.1 S2O82-
-
 Colwellia psychrerythraea
4.2.1.1 SCN-
-
 Colwellia psychrerythraea
4.2.1.1 SeCN-
-
 Colwellia psychrerythraea
4.2.1.1 SeO42-
-
 Colwellia psychrerythraea
4.2.1.1 SnO32-
-
 Colwellia psychrerythraea
4.2.1.1 SO42-
-
 Colwellia psychrerythraea
4.2.1.1 TeO42-
-
 Colwellia psychrerythraea
4.2.1.1 V2O74-
-
 Colwellia psychrerythraea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.1 4700
-
 CO2 pH 8.3, 22°C Colwellia psychrerythraea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.1 Zn2+ required, metalloenzyme. The three amino acid residues Asn73, Gln75 and Asn202, which constitute the triad involved in the network of hydrogen bonds with the zinc-coordinated water molecule, are conserved in all the sequences of gamma-CA enzymes. The enzyme CpsCA trimer contains 3 active sites and each monomer contributes His residues located on the surface to coordinate the Zn(II) ion crucial for catalysis Colwellia psychrerythraea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.1 55000
-
 recombinant enzyme, native PAGE Colwellia psychrerythraea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.1 CO2 + H2O Colwellia psychrerythraea
-
 H2CO3
-
 r
4.2.1.1 CO2 + H2O Colwellia psychrerythraea 34H / ATCC BAA-681
-
 H2CO3
-
 r
4.2.1.1 H2CO3 Colwellia psychrerythraea
-
 CO2 + H2O
-
 r
4.2.1.1 H2CO3 Colwellia psychrerythraea 34H / ATCC BAA-681
-
 CO2 + H2O
-
 r
4.2.1.1 additional information Colwellia psychrerythraea the enzyme shows a significant catalytic activity for the physiologic reaction of CO2 hydration to bicarbonate and protons ?
-
 ?
4.2.1.1 additional information Colwellia psychrerythraea 34H / ATCC BAA-681 the enzyme shows a significant catalytic activity for the physiologic reaction of CO2 hydration to bicarbonate and protons ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.1 Colwellia psychrerythraea Q47YG3 i.e. Vibrio psychroerythus
-
4.2.1.1 Colwellia psychrerythraea 34H / ATCC BAA-681 Q47YG3 i.e. Vibrio psychroerythus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.1 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)/ArcticExpress DE3 cells by nickel affinity chromatography Colwellia psychrerythraea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.1 CO2 + H2O
-
 Colwellia psychrerythraea H2CO3
-
 r
4.2.1.1 CO2 + H2O
-
 Colwellia psychrerythraea 34H / ATCC BAA-681 H2CO3
-
 r
4.2.1.1 H2CO3
-
 Colwellia psychrerythraea CO2 + H2O
-
 r
4.2.1.1 H2CO3
-
 Colwellia psychrerythraea 34H / ATCC BAA-681 CO2 + H2O
-
 r
4.2.1.1 additional information the enzyme shows a significant catalytic activity for the physiologic reaction of CO2 hydration to bicarbonate and protons Colwellia psychrerythraea ?
-
 ?
4.2.1.1 additional information the enzyme shows a significant catalytic activity for the physiologic reaction of CO2 hydration to bicarbonate and protons Colwellia psychrerythraea 34H / ATCC BAA-681 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.1 homotrimer 3 * 20000, recombinant enzyme, SDS-PAGE Colwellia psychrerythraea
4.2.1.1 More cross-sectional profiles of the gamma-CA trimer reveal that each monomer resembles an equilateral triangle, which is stabilized by hydrogen bonds, salt bridges and hydrophobic interactions. The trimer contains 3 active sites and each monomer contributes His residues located on the surface to coordinate the Zn(II) ion crucial for catalysis Colwellia psychrerythraea

Synonyms

EC Number Synonyms Comment Organism
4.2.1.1 CpsCA
-
 Colwellia psychrerythraea
4.2.1.1 CpsCAgamma
-
 Colwellia psychrerythraea
4.2.1.1 gamma-CA
-
 Colwellia psychrerythraea
4.2.1.1 gamma-carbonic anhydrase
-
 Colwellia psychrerythraea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.1 22
-
 assay at room temperature Colwellia psychrerythraea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.1 600000
-
 CO2 pH 8.3, 22°C Colwellia psychrerythraea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.1 8.3
-
 assay at Colwellia psychrerythraea

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.1 0.000502
-
 acetazolamide pH 8.3, 22°C Colwellia psychrerythraea
4.2.1.1 0.008
-
 H2NSO2NH2 pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
4.2.1.1 0.026
-
 Ph-B(OH)2 pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
4.2.1.1 0.037
-
 H2NSO3H pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
4.2.1.1 0.038
-
 Ph-AsO3H2 pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
4.2.1.1 0.58
-
 Et2NCS2- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
4.2.1.1 1.4
-
 CO32- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
4.2.1.1 1.7
-
 HCO3- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
4.2.1.1 1.8
-
 SnO32- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
4.2.1.1 2.5
-
 CNO- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
4.2.1.1 3.3
-
 SCN- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
4.2.1.1 3.8
-
 CS32- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
4.2.1.1 4.3
-
 FSO3- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
4.2.1.1 4.4
-
 CN- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
4.2.1.1 4.5
-
 S2O82- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
4.2.1.1 4.7
-
 B4O72- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
4.2.1.1 4.8
-
 HS- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
4.2.1.1 5.1
-
 TeO42- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
4.2.1.1 5.6
-
 PF6- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
4.2.1.1 5.8
-
 V2O74- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
4.2.1.1 6
-
 SeCN- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
4.2.1.1 7.3
-
 P2O74- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
4.2.1.1 7.7
-
 CF3SO3- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
4.2.1.1 7.8
-
 ReO4- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
4.2.1.1 7.9
-
 RuO4- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
4.2.1.1 7.9
-
 NH(SO3)22- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
4.2.1.1 8.3
-
 SeO42- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
4.2.1.1 8.7
-
 I- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
4.2.1.1 8.8
-
 NO2- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
4.2.1.1 9
-
 Cl- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
4.2.1.1 9.4
-
 SO42- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
4.2.1.1 21.7
-
 NO3- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea
4.2.1.1 37.5
-
 HSO3- pH 8.3, 22°C, inhibition of CO2 hydration activity Colwellia psychrerythraea