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Literature summary extracted from

  • Toporkova, Y.Y.; Gorina, S.S.; Bessolitsyna, E.K.; Smirnova, E.O.; Fatykhova, V.S.; Bruehlmann, F.; Ilyina, T.M.; Mukhtarova, L.S.; Grechkin, A.N.
    Double function hydroperoxide lyases/epoxyalcohol synthases (CYP74C) of higher plants identification and conversion into allene oxide synthases by site-directed mutagenesis (2018), Biochim. Biophys. Acta, 1863, 369-378 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.92 CYP74C13_MT gene, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta-gami(DE3)pLysS B Glycine max
4.2.1.92 CYP74C13_MT gene, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)pLysS Cucumis sativus
4.2.1.92 CYP74C13_MT gene, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain Rosetta-gami(DE3)pLysS B Medicago truncatula
4.2.1.92 CYP74C1_CS gene, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged wild-type enzyme in Escherichia coli strain BL21(DE3)pLysS Cucumis sativus
4.2.1.92 CYP74C4_ST gene, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged wild-type enzyme in Escherichia coli strain BL21(DE3)pLysS Solanum tuberosum
4.2.1.92 sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS Cucumis melo

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.92 L93F/G283A site-directed mutagenesis, the mutant shows altered substrate specificity and increased activity compared to the wild-type enzyme Solanum tuberosum
4.2.1.92 L93F/G283A site-directed mutagenesis, the mutant shows altered substrate specificity and increased activity compared to the wild-type enzyme. The mutant form preferentially utilise (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid Cucumis sativus
4.2.1.92 L97F site-directed mutagenesis, L97F/TCFNSF, multiple mutations of the hydroperoxide-binding domain leading to the TCFNSF motif virtually do not alter the specificity of catalysis compared to the wild-type enzyme. Conversely, the L97F variant offers good allene oxide synthase (AOS) activity with (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid (9-HPOD). The mutant form preferentially utilise (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid Medicago truncatula
4.2.1.92 L97F site-directed mutagenesis, the mutant shows altered substrate specificity and decreased activity compared to the wild-type enzyme Solanum tuberosum
4.2.1.92 L98F/A287G site-directed mutagenesis, the mutant shows altered substrate specificity and increased activity compared to the wild-type enzyme Solanum tuberosum
4.2.1.92 L98F/A287G site-directed mutagenesis, the mutant shows altered substrate specificity and increased activity compared to the wild-type enzyme. The mutant form preferentially utilise (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid Cucumis sativus
4.2.1.92 additional information reaction products are identified and quantified using HPLC chromatography and mass spectrometry Cucumis sativus
4.2.1.92 additional information reaction products are identified and quantified using HPLC chromatography and mass spectrometry Medicago truncatula
4.2.1.92 additional information reaction products are identified and quantified using HPLC chromatography and mass spectrometry. All three mutant forms preferentially utilise (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid. Multiple mutations of the hydroperoxide-binding domain leading to the TCFNSF motif virtually did not alter the specificity of catalysis compared to the wild-type enzyme. Conversely, the L97F variant offers good allene oxide synthase (AOS) activity with (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid (9-HPOD) Solanum tuberosum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.92 (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid Cucumis sativus
-
 (9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
4.2.1.92 (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid Glycine max
-
 (9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
4.2.1.92 (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid Solanum tuberosum
-
 (9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
4.2.1.92 (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid Medicago truncatula
-
 (9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
4.2.1.92 (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid Cucumis sativus
-
 (9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid + 9-hydroxy-10-oxo-12-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + H2O + ?
-
 ?
4.2.1.92 (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid Cucumis melo
-
 (9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid + 9-hydroxy-10-oxo-12-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + H2O + ?
-
 ?
4.2.1.92 (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid Cucumis sativus
-
 9-hydroxynonanoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
4.2.1.92 (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid Glycine max
-
 9-hydroxynonanoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
4.2.1.92 (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid Medicago truncatula
-
 9-hydroxynonanoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
4.2.1.92 (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid Solanum tuberosum
-
 (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
4.2.1.92 (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid Cucumis sativus
-
 (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
4.2.1.92 (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid Cucumis melo
-
 (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
4.2.1.92 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid Solanum tuberosum
-
 (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ?
-
 ?
4.2.1.92 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid Cucumis sativus
-
 11-hydroxy-12,13-epoxy-9-octadecenoic acid + 9-hydroxy-12,13-epoxy-10-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ?
-
 ?
4.2.1.92 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid Glycine max
-
 11-hydroxy-12,13-epoxy-9-octadecenoic acid + 9-hydroxy-12,13-epoxy-10-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ?
-
 ?
4.2.1.92 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid Cucumis melo
-
 11-hydroxy-12,13-epoxy-9-octadecenoic acid + 9-hydroxy-12,13-epoxy-10-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ?
-
 ?
4.2.1.92 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid Medicago truncatula
-
 11-hydroxy-12,13-epoxy-9-octadecenoic acid + 9-hydroxy-12,13-epoxy-10-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ?
-
 ?
4.2.1.92 (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid Cucumis sativus
-
 (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ?
-
 ?
4.2.1.92 (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid Glycine max
-
 (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ?
-
 ?
4.2.1.92 (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid Cucumis melo
-
 (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ?
-
 ?
4.2.1.92 (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid Medicago truncatula
-
 (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ?
-
 ?
4.2.1.92 additional information Cucumis sativus reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specificities, overview ?
-
 ?
4.2.1.92 additional information Cucumis melo reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specificities, overview ?
-
 ?
4.2.1.92 additional information Glycine max reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specifities, overview ?
-
 ?
4.2.1.92 additional information Solanum tuberosum reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specifities, overview ?
-
 ?
4.2.1.92 additional information Cucumis sativus reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specifities, overview ?
-
 ?
4.2.1.92 additional information Medicago truncatula reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specifities, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.92 Cucumis melo Q93XR3
-
-
4.2.1.92 Cucumis sativus A0A0A0K2U8
-
-
4.2.1.92 Cucumis sativus U5YTM6
-
-
4.2.1.92 Glycine max M4WFN0
-
-
4.2.1.92 Medicago truncatula Q7X9B3
-
-
4.2.1.92 Solanum tuberosum C7ENW3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.92 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography Cucumis melo
4.2.1.92 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography Cucumis sativus
4.2.1.92 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta-gami(DE3)pLysS B by nickel affinity chromatography Medicago truncatula
4.2.1.92 recombinant His-tagged wild-type enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography Cucumis sativus
4.2.1.92 recombinant His-tagged wild-type enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography Solanum tuberosum
4.2.1.92 recombinant His-tagged wild-type enzyme from Escherichia coli strain Rosetta-gami(DE3)pLysS B by nickel affinity chromatography Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.92 (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid
-
 Cucumis sativus (9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
4.2.1.92 (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid
-
 Glycine max (9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
4.2.1.92 (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid
-
 Solanum tuberosum (9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
4.2.1.92 (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid
-
 Medicago truncatula (9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
4.2.1.92 (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid
-
 Cucumis sativus (9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid + 9-hydroxy-10-oxo-12-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + H2O + ?
-
 ?
4.2.1.92 (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid
-
 Cucumis melo (9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid + 9-hydroxy-10-oxo-12-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + H2O + ?
-
 ?
4.2.1.92 (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid
-
 Cucumis sativus 9-hydroxynonanoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
4.2.1.92 (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid
-
 Glycine max 9-hydroxynonanoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
4.2.1.92 (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid
-
 Medicago truncatula 9-hydroxynonanoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
4.2.1.92 (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid
-
 Solanum tuberosum (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
4.2.1.92 (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid
-
 Cucumis sativus (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
4.2.1.92 (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid
-
 Cucumis melo (9Z)-12-hydroxy-9-dodecenoic acid + ?
-
 ?
4.2.1.92 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid
-
 Solanum tuberosum (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ?
-
 ?
4.2.1.92 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid
-
 Cucumis sativus 11-hydroxy-12,13-epoxy-9-octadecenoic acid + 9-hydroxy-12,13-epoxy-10-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ?
-
 ?
4.2.1.92 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid
-
 Glycine max 11-hydroxy-12,13-epoxy-9-octadecenoic acid + 9-hydroxy-12,13-epoxy-10-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ?
-
 ?
4.2.1.92 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid
-
 Cucumis melo 11-hydroxy-12,13-epoxy-9-octadecenoic acid + 9-hydroxy-12,13-epoxy-10-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ?
-
 ?
4.2.1.92 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid
-
 Medicago truncatula 11-hydroxy-12,13-epoxy-9-octadecenoic acid + 9-hydroxy-12,13-epoxy-10-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ?
-
 ?
4.2.1.92 (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid
-
 Cucumis sativus (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ?
-
 ?
4.2.1.92 (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid
-
 Glycine max (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ?
-
 ?
4.2.1.92 (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid
-
 Cucumis melo (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ?
-
 ?
4.2.1.92 (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid
-
 Medicago truncatula (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ?
-
 ?
4.2.1.92 additional information reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specificities, overview Cucumis sativus ?
-
 ?
4.2.1.92 additional information reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specificities, overview Cucumis melo ?
-
 ?
4.2.1.92 additional information reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specifities, overview Cucumis sativus ?
-
 ?
4.2.1.92 additional information reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specifities, overview Glycine max ?
-
 ?
4.2.1.92 additional information reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specifities, overview Solanum tuberosum ?
-
 ?
4.2.1.92 additional information reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specifities, overview Medicago truncatula ?
-
 ?
4.2.1.92 additional information no activity with (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid Solanum tuberosum ?
-
 ?
4.2.1.92 additional information reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specifities, overview. No activity with (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid Solanum tuberosum ?
-
 ?
4.2.1.92 additional information reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specifities, overview. No activity with (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid Cucumis melo ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.92 More three-dimensional modelling of CYP74s and structure comparisons Cucumis sativus
4.2.1.92 More three-dimensional modelling of CYP74s and structure comparisons Glycine max
4.2.1.92 More three-dimensional modelling of CYP74s and structure comparisons Solanum tuberosum
4.2.1.92 More three-dimensional modelling of CYP74s and structure comparisons Cucumis melo
4.2.1.92 More three-dimensional modelling of CYP74s and structure comparisons Medicago truncatula

Synonyms

EC Number Synonyms Comment Organism
4.2.1.92 9/13 hydroperoxide lyase
-
 Medicago truncatula
4.2.1.92 CmHPL
-
 Cucumis melo
4.2.1.92 Csa_7G075590
-
 Cucumis sativus
4.2.1.92 CYP74C13_GM
-
 Glycine max
4.2.1.92 CYP74C13_MT
-
 Medicago truncatula
4.2.1.92 CYP74C1_CS
-
 Cucumis sativus
4.2.1.92 CYP74C2
-
 Cucumis melo
4.2.1.92 CYP74C31
-
 Cucumis sativus
4.2.1.92 CYP74C4_ST
-
 Solanum tuberosum
4.2.1.92 fatty acid 9-hydroperoxide lyase UniProt Cucumis sativus
4.2.1.92 fatty acid 9-hydroperoxide lyase UniProt Cucumis melo
4.2.1.92 fatty acid hydroperoxide lyase UniProt Solanum tuberosum
4.2.1.92 fatty acid hydroperoxide lyase UniProt Medicago truncatula
4.2.1.92 HPL
-
 Cucumis sativus
4.2.1.92 HPL
-
 Glycine max
4.2.1.92 HPL
-
 Solanum tuberosum
4.2.1.92 HPL
-
 Cucumis melo
4.2.1.92 HPL
-
 Medicago truncatula
4.2.1.92 HPL1
-
 Cucumis sativus
4.2.1.92 HPL1
-
 Medicago truncatula
4.2.1.92 hydroperoxide lyase
-
 Cucumis sativus
4.2.1.92 hydroperoxide lyase
-
 Glycine max
4.2.1.92 hydroperoxide lyase
-
 Solanum tuberosum
4.2.1.92 hydroperoxide lyase
-
 Cucumis melo
4.2.1.92 hydroperoxide lyase
-
 Medicago truncatula
4.2.1.92 MtrunA17_Chr4g0034141
-
 Medicago truncatula

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.92 23
-
 assay at Cucumis sativus
4.2.1.92 23
-
 assay at Glycine max
4.2.1.92 23
-
 assay at Solanum tuberosum
4.2.1.92 23
-
 assay at Cucumis melo
4.2.1.92 23
-
 assay at Medicago truncatula

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.92 173.8
-
 (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid pH 7.0, 23°C, recombinant wild-type enzyme Glycine max
4.2.1.92 187.1
-
 (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid pH 7.0, 23°C, recombinant mutant L97F/TCFNSF enzyme Medicago truncatula
4.2.1.92 228.6
-
 (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid pH 7.0, 23°C, recombinant wild-type enzyme Glycine max
4.2.1.92 290
-
 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid pH 7.0, 23°C, recombinant wild-type enzyme Glycine max
4.2.1.92 337.6
-
 (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid pH 7.0, 23°C, recombinant wild-type enzyme Cucumis sativus
4.2.1.92 356.1
-
 (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid pH 7.0, 23°C, recombinant mutant L98F/A287G enzyme Cucumis sativus
4.2.1.92 375.4
-
 (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid pH 7.0, 23°C, recombinant wild-type enzyme Cucumis sativus
4.2.1.92 377.2
-
 (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid pH 7.0, 23°C, recombinant wild-type enzyme Cucumis sativus
4.2.1.92 455.3
-
 (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid pH 7.0, 23°C, recombinant wild-type enzyme Cucumis sativus
4.2.1.92 471.4
-
 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid pH 7.0, 23°C, recombinant mutant L97F/TCFNSF enzyme Medicago truncatula
4.2.1.92 530.9
-
 (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid pH 7.0, 23°C, recombinant wild-type enzyme Cucumis sativus
4.2.1.92 550
-
 (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid pH 7.0, 23°C, recombinant mutant L97F/TCFNSF enzyme Medicago truncatula
4.2.1.92 571.4
-
 (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid pH 7.0, 23°C, recombinant mutant L97F/TCFNSF enzyme Medicago truncatula
4.2.1.92 596.4
-
 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid pH 7.0, 23°C, recombinant wild-type enzyme Cucumis sativus
4.2.1.92 600
-
 (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid pH 7.0, 23°C, recombinant wild-type enzyme Solanum tuberosum
4.2.1.92 619.8
-
 (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid pH 7.0, 23°C, recombinant mutant L93F/G283A enzyme Cucumis sativus
4.2.1.92 676.4
-
 (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid pH 7.0, 23°C, recombinant wild-type enzyme Cucumis sativus
4.2.1.92 712.7
-
 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid pH 7.0, 23°C, recombinant mutant L93F/G283A enzyme Cucumis sativus
4.2.1.92 732.4
-
 (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid pH 7.0, 23°C, recombinant mutant L93F/G283A enzyme Cucumis sativus
4.2.1.92 752.4
-
 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid pH 7.0, 23°C, recombinant wild-type enzyme Cucumis sativus
4.2.1.92 768.9
-
 (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid pH 7.0, 23°C, recombinant wild-type enzyme Medicago truncatula
4.2.1.92 773.3
-
 (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid pH 7.0, 23°C, recombinant wild-type enzyme Medicago truncatula
4.2.1.92 829.1
-
 (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid pH 7.0, 23°C, recombinant mutant L93F/G283A enzyme Cucumis sativus
4.2.1.92 867.7
-
 (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid pH 7.0, 23°C, recombinant wild-type enzyme Medicago truncatula
4.2.1.92 913.3
-
 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid pH 7.0, 23°C, recombinant wild-type enzyme Medicago truncatula
4.2.1.92 949.6
-
 (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid pH 7.0, 23°C, recombinant mutant L98F/A287G enzyme Cucumis sativus
4.2.1.92 997
-
 (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid pH 7.0, 23°C, recombinant mutant L98F/A287G enzyme Cucumis sativus
4.2.1.92 1011.6
-
 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid pH 7.0, 23°C, recombinant wild-type enzyme Solanum tuberosum
4.2.1.92 1115.9
-
 (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid pH 7.0, 23°C, recombinant wild-type enzyme Solanum tuberosum
4.2.1.92 1587.5
-
 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid pH 7.0, 23°C, recombinant mutant L98F/A287G enzyme Cucumis sativus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.92 7
-
 assay at, the reaction mixture acidifies to pH 6.0 Cucumis sativus
4.2.1.92 7
-
 assay at, the reaction mixture acidifies to pH 6.0 Glycine max
4.2.1.92 7
-
 assay at, the reaction mixture acidifies to pH 6.0 Solanum tuberosum
4.2.1.92 7
-
 assay at, the reaction mixture acidifies to pH 6.0 Cucumis melo
4.2.1.92 7
-
 assay at, the reaction mixture acidifies to pH 6.0 Medicago truncatula

General Information

EC Number General Information Comment Organism
4.2.1.92 evolution hydroxyperoxide lyases belong to the CYP74C subfamily of fatty acid hydroperoxide transforming enzymes, unrooted phylogenetic tree of CYP74 family with special emphasis to CYP74C subfamily, overview Cucumis sativus
4.2.1.92 evolution hydroxyperoxide lyases belong to the CYP74C subfamily of fatty acid hydroperoxide transforming enzymes, unrooted phylogenetic tree of CYP74 family with special emphasis to CYP74C subfamily, overview Glycine max
4.2.1.92 evolution hydroxyperoxide lyases belong to the CYP74C subfamily of fatty acid hydroperoxide transforming enzymes, unrooted phylogenetic tree of CYP74 family with special emphasis to CYP74C subfamily, overview Solanum tuberosum
4.2.1.92 evolution hydroxyperoxide lyases belong to the CYP74C subfamily of fatty acid hydroperoxide transforming enzymes, unrooted phylogenetic tree of CYP74 family with special emphasis to CYP74C subfamily, overview Cucumis melo
4.2.1.92 evolution hydroxyperoxide lyases belong to the CYP74C subfamily of fatty acid hydroperoxide transforming enzymes, unrooted phylogenetic tree of CYP74 family with special emphasis to CYP74C subfamily, overview Medicago truncatula
4.2.1.92 malfunction the mutant enzyme preferentially utilises 9-hydroperoxylinoleic acid Cucumis sativus
4.2.1.92 malfunction the mutant enzyme preferentially utilises 9-hydroperoxylinoleic acid. Multiple mutations of the hydroperoxide-binding domain leading to the TCFNSF motif virtually do not alter the specificity of catalysis compared to the wild-type enzyme. Conversely, the L97F variant offers good allene oxide synthase (AOS) activity with 9-hydroperoxylinoleic acid (9-HPOD) Medicago truncatula
4.2.1.92 additional information three-dimensional modelling of CYP74s and structure comparisons Cucumis sativus
4.2.1.92 additional information three-dimensional modelling of CYP74s and structure comparisons Glycine max
4.2.1.92 additional information three-dimensional modelling of CYP74s and structure comparisons Solanum tuberosum
4.2.1.92 additional information three-dimensional modelling of CYP74s and structure comparisons Cucumis melo