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Literature summary extracted from

  • Ortega-Ramos, M.; Canniffe, D.; Radle, M.; Neil Hunter, C.; Bryant, D.; Golbeck, J.
    Engineered biosynthesis of bacteriochlorophyll gF in Rhodobacter sphaeroides (2018), Biochim. Biophys. Acta, 1859, 501-509 .
No PubMed abstract available

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.165 additional information deletion of gene bchF in a BChl b-producing strain of Rhodobacter sphaeroides leads to the production of an analogue of bacteriochlorophyllide g, BChl gP, where P is phytyl, rather than the native BChl aP. In the bchF-deletion mutant, hydration of the C3-vinyl group in 3-vinyl bacteriochlorophyllide a is blocked, but this precursor can be esterified with phytol. Enzyme BchF deletion- in the DELTAbciA/bchXYZBv background results in formation of BChl g esterified with phytol (BChl gP) Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.165 3-vinyl bacteriochlorophyllide a + H2O Cereibacter sphaeroides
-
 3-(1-hydroxyethyl) bacteriochlorophyllide a
-
 r
4.2.1.165 bacteriochlorophyllide g + H2O Cereibacter sphaeroides
-
 3-(1-hydroxyethyl) bacteriochlorophyllide g
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.165 Cereibacter sphaeroides Q53222
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.165 3-vinyl bacteriochlorophyllide a + H2O
-
 Cereibacter sphaeroides 3-(1-hydroxyethyl) bacteriochlorophyllide a
-
 r
4.2.1.165 bacteriochlorophyllide g + H2O
-
 Cereibacter sphaeroides 3-(1-hydroxyethyl) bacteriochlorophyllide g
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.2.1.165 2-vinyl bacteriochlorophyllide hydratase UniProt Cereibacter sphaeroides
4.2.1.165 bchF
-
 Cereibacter sphaeroides
4.2.1.165 BchF hydratase
-
 Cereibacter sphaeroides

General Information

EC Number General Information Comment Organism
4.2.1.165 malfunction deletion of gene bchF in a bacteriochlorophyll (BChl) b-producing strain of Rhodobacter sphaeroides leads to the production of an analogue of bacteriochlorophyllide g, BChl gP, where P is phytyl, rather than the native BChl aP. In the bchF-deletion mutant, hydration of the C3-vinyl group in 3-vinyl bacteriochlorophyllide a is blocked, but this precursor can be esterified with phytol. Enzyme BchF deletion- in the DELTAbciA/bchXYZBv background results in formation of BChl g esterified with phytol (BChl gP). Pigment analysis in several bch mutants, overview Cereibacter sphaeroides
4.2.1.165 metabolism the enzyme is involved in the biosynthetic pathways of different baceriochlorophyllides, e.g. a and g, overview Cereibacter sphaeroides
4.2.1.165 physiological function gene bchF encodes an enzyme responsible for the hydration of the C3-vinyl group of a precursor of bacteriochlorophylls Cereibacter sphaeroides