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Literature summary extracted from

  • Anufrieva, N.V.; Faleev, N.G.; Morozova, E.A.; Bazhulina, N.P.; Revtovich, S.V.; Timofeev, V.P.; Tkachev, Y.V.; Nikulin, A.D.; Demidkina, T.V.
    The role of active site tyrosine 58 in Citrobacter freundii methionine gamma-lyase (2015), Biochim. Biophys. Acta, 1854, 1220-1228 .
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.4.1.11 structure of mutant Y58F, to 1.96 A resolution. The mutation does not result in essential changes of the conformation of the active site Citrobacter freundii

Protein Variants

EC Number Protein Variants Comment Organism
4.4.1.11 Y58F mutation leads to a decrease of catalytic efficiencies in both gamma- and beta-elimination reactions of about two orders of magnitude and to a change of rate the limiting step of the physiological reaction Citrobacter freundii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.11 2.75
-
 S-Benzyl-L-cysteine mutant Y58F, pH 8.0, 30°C Citrobacter freundii
4.4.1.11 3.46
-
 DL-homocysteine mutant Y58F, pH 8.0, 30°C Citrobacter freundii
4.4.1.11 8.11
-
 S-ethyl-L-homocysteine mutant Y58F, pH 8.0, 30°C Citrobacter freundii
4.4.1.11 8.19
-
 S-ethyl-L-cysteine mutant Y58F, pH 8.0, 30°C Citrobacter freundii
4.4.1.11 19.65
-
 L-methionine mutant Y58F, pH 8.0, 30°C Citrobacter freundii

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.11 Citrobacter freundii Q84AR1
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.11 3.25
-
 mutant Y58F, pH 8.0, 30°C Citrobacter freundii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.11 DL-homocysteine + H2O
-
 Citrobacter freundii 2-oxobutanoate + NH3 + hydrogen sulfide
-
 ?
4.4.1.11 L-methionine + H2O
-
 Citrobacter freundii methanethiol + NH3 + 2-oxobutanoate
-
 ?
4.4.1.11 S-benzyl-L-cysteine + H2O
-
 Citrobacter freundii thiobenzyl alcohol + NH3 + 2-oxopropanoate
-
 ?
4.4.1.11 S-ethyl-L-cysteine + H2O
-
 Citrobacter freundii ethanethiol + NH3 + 2-oxopropanoate
-
 ?
4.4.1.11 S-ethyl-L-homocysteine + H2O
-
 Citrobacter freundii ethanethiol + NH3 + 2-oxobutanoate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4.1.11 0.88
-
 DL-homocysteine mutant Y58F, pH 8.0, 30°C Citrobacter freundii
4.4.1.11 2.07
-
 S-ethyl-L-homocysteine mutant Y58F, pH 8.0, 30°C Citrobacter freundii
4.4.1.11 2.38
-
 S-ethyl-L-cysteine mutant Y58F, pH 8.0, 30°C Citrobacter freundii
4.4.1.11 2.66
-
 L-methionine mutant Y58F, pH 8.0, 30°C Citrobacter freundii
4.4.1.11 3.34
-
 S-Benzyl-L-cysteine mutant Y58F, pH 8.0, 30°C Citrobacter freundii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.4.1.11 0.121
-
 S-Benzyl-L-cysteine mutant Y58F, pH 8.0, 30°C Citrobacter freundii
4.4.1.11 0.135
-
 L-methionine mutant Y58F, pH 8.0, 30°C Citrobacter freundii
4.4.1.11 0.254
-
 DL-homocysteine mutant Y58F, pH 8.0, 30°C Citrobacter freundii
4.4.1.11 0.255
-
 S-ethyl-L-homocysteine mutant Y58F, pH 8.0, 30°C Citrobacter freundii
4.4.1.11 0.291
-
 S-ethyl-L-cysteine mutant Y58F, pH 8.0, 30°C Citrobacter freundii