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Literature summary extracted from

  • Andrews, F.H.; Horton, J.D.; Shin, D.; Yoon, H.J.; Logsdon, M.G.; Malik, A.M.; Rogers, M.P.; Kneen, M.M.; Suh, S.W.; McLeish, M.J.
    The kinetic characterization and X-ray structure of a putative benzoylformate decarboxylase from M. smegmatis highlights the difficulties in the functional annotation of ThDP-dependent enzymes (2015), Biochim. Biophys. Acta, 1854, 1001-1009 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.7 expressed in Escherichia coli Rosetta2(DE3)pLysS cells Mycolicibacterium smegmatis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.7 sitting drop vapor diffusion method, using 4.0 M sodium formate Mycolicibacterium smegmatis

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.7 I466A the mutant shows increased catalytic efficiency for benzoylformate compared to the wild type enzyme Mycolicibacterium smegmatis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.7 2 3 benzoylformate wild type enzyme, at pH 6.0 and 30°C Mycolicibacterium smegmatis
4.1.1.7 15
-
 benzoylformate mutant enzyme I466A, at pH 6.0 and 30°C Mycolicibacterium smegmatis
4.1.1.7 21
-
 2-Oxohexanoate wild type enzyme, at pH 6.0 and 30°C Mycolicibacterium smegmatis
4.1.1.7 21
-
 4-methylthio-2-oxobutanoate wild type enzyme, at pH 6.0 and 30°C Mycolicibacterium smegmatis

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.7 Mycolicibacterium smegmatis
-
-
-
4.1.1.7 Mycolicibacterium smegmatis mc2155
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.7 HiTrap Ni2+ chelating column chromatography Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.7 2-oxohexanoate best substrate Mycolicibacterium smegmatis valeraldehyde + CO2
-
 ?
4.1.1.7 2-oxohexanoate best substrate Mycolicibacterium smegmatis mc2155 valeraldehyde + CO2
-
 ?
4.1.1.7 4-methylthio-2-oxobutanoate
-
 Mycolicibacterium smegmatis 3-(methylsulfanyl)propanal + CO2
-
 ?
4.1.1.7 4-methylthio-2-oxobutanoate
-
 Mycolicibacterium smegmatis mc2155 3-(methylsulfanyl)propanal + CO2
-
 ?
4.1.1.7 benzoylformate
-
 Mycolicibacterium smegmatis benzaldehyde + CO2
-
 ?
4.1.1.7 benzoylformate
-
 Mycolicibacterium smegmatis mc2155 benzaldehyde + CO2
-
 ?
4.1.1.7 additional information very low activity with pyruvate, phenylpyruvate, 2-2oxopentanoate, 3-methyl-2-oxopentanoate, 2-oxobutanoate, and 3-methyl-2-oxobutanoate. No activity with 2-oxoglutarate Mycolicibacterium smegmatis ?
-
 ?
4.1.1.7 additional information very low activity with pyruvate, phenylpyruvate, 2-2oxopentanoate, 3-methyl-2-oxopentanoate, 2-oxobutanoate, and 3-methyl-2-oxobutanoate. No activity with 2-oxoglutarate Mycolicibacterium smegmatis mc2155 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.7 BFDC
-
 Mycolicibacterium smegmatis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.7 4.6
-
 benzoylformate mutant enzyme I466A, at pH 6.0 and 30°C Mycolicibacterium smegmatis
4.1.1.7 5.1
-
 benzoylformate wild type enzyme, at pH 6.0 and 30°C Mycolicibacterium smegmatis
4.1.1.7 20
-
 2-Oxohexanoate wild type enzyme, at pH 6.0 and 30°C Mycolicibacterium smegmatis
4.1.1.7 20
-
 4-methylthio-2-oxobutanoate wild type enzyme, at pH 6.0 and 30°C Mycolicibacterium smegmatis

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.7 thiamine diphosphate
-
 Mycolicibacterium smegmatis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.1.7 0.22
-
 benzoylformate wild type enzyme, at pH 6.0 and 30°C Mycolicibacterium smegmatis
4.1.1.7 0.3
-
 benzoylformate mutant enzyme I466A, at pH 6.0 and 30°C Mycolicibacterium smegmatis
4.1.1.7 0.94
-
 2-Oxohexanoate wild type enzyme, at pH 6.0 and 30°C Mycolicibacterium smegmatis
4.1.1.7 0.95
-
 4-methylthio-2-oxobutanoate wild type enzyme, at pH 6.0 and 30°C Mycolicibacterium smegmatis