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Literature summary extracted from

  • Pizzuto, R.; Paventi, G.; Porcile, C.; Sarnataro, D.; Daniele, A.; Passarella, S.
    L-Lactate metabolism in HEP G2 cell mitochondria due to the L-lactate dehydrogenase determines the occurrence of the lactate/pyruvate shuttle and the appearance of oxaloacetate, malate and citrate outside mitochondria (2012), Biochim. Biophys. Acta, 1817, 1679-1690 .
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.27 additional information
-
 additional information Km values for pyruvate, NADH, (S)-lactate and NAD+ are measured in the solubilized mirochondrial Hep G2 fractions. They differ from the values measured in the cytosolic fractions Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.27 mitochondrion L-lactate dehydrogenase is restricted to the inner mitochondrial compartments Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.27 (S)-lactate + NAD+ Homo sapiens since the mitochondrial metabolism of (S)-lactate results in synthesis and export of oxaloacetate, malate and citrate into the extramitochondrial phase, an anaplerotic role for the mitochondrial (S)-lactate metabolism is proposed pyruvate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.27 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.27 Hep-G2 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.27 (S)-lactate + NAD+
-
 Homo sapiens pyruvate + NADH + H+
-
 r
1.1.1.27 (S)-lactate + NAD+ since the mitochondrial metabolism of (S)-lactate results in synthesis and export of oxaloacetate, malate and citrate into the extramitochondrial phase, an anaplerotic role for the mitochondrial (S)-lactate metabolism is proposed Homo sapiens pyruvate + NADH + H+
-
 r
1.1.1.27 pyruvate + NADH + H+
-
 Homo sapiens (S)-lactate + NAD+
-
 r

General Information

EC Number General Information Comment Organism
1.1.1.27 metabolism since the mitochondrial metabolism of (S)-lactate results in synthesis and export of oxaloacetate, malate and citrate into the extramitochondrial phase, an anaplerotic role for the mitochondrial (S)-lactate metabolism is proposed Homo sapiens