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Literature summary extracted from

  • Zhu, W.; Reinhardt, L.A.; Richards, N.G.J.
    Second-shell hydrogen bond impacts transition-state structure in Bacillus subtilis oxalate decarboxylase (2018), Biochemistry, 57, 3425-3432 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.2 expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.2 W132F the mutant with increased manganese content shows reduced activity compared to the wild type enzyme Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.2 4
-
 oxalate wild type enzyme, at pH 4.2 and 25°C Bacillus subtilis
4.1.1.2 27
-
 oxalate mutant enzyme W132F, at pH 4.2 and 25°C Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.2 Mn2+ dependent on Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.2 oxalate + H+ Bacillus subtilis
-
 formate + CO2
-
 ?
4.1.1.2 oxalate + H+ Bacillus subtilis 168
-
 formate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.2 Bacillus subtilis O34714
-
-
4.1.1.2 Bacillus subtilis 168 O34714
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.2 Ni-NTA column chromatography Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.2 oxalate + H+
-
 Bacillus subtilis formate + CO2
-
 ?
4.1.1.2 oxalate + H+
-
 Bacillus subtilis 168 formate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.2 ? x * 44000, SDS-PAGE Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
4.1.1.2 OXDC
-
 Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.2 60
-
 oxalate mutant enzyme W132F, at pH 4.2 and 25°C Bacillus subtilis
4.1.1.2 60
-
 oxalate wild type enzyme, at pH 4.2 and 25°C Bacillus subtilis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.1.2 1.3
-
 oxalate mutant enzyme W132F, at pH 4.2 and 25°C Bacillus subtilis
4.1.1.2 10
-
 oxalate wild type enzyme, at pH 4.2 and 25°C Bacillus subtilis