BRENDA - Enzyme Database

The dimethylsulfoniopropionate (DMSP) lyase and lyase-like cupin family consists of bona fide DMSP lyases as well as other enzymes with unknown function

Lei, L.; Cherukuri, K.P.; Alcolombri, U.; Meltzer, D.; Tawfik, D.S.; Biochemistry 57, 3364-3377 (2018)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.4.1.3
expression in Escherichia coli
Alcaligenes faecalis
4.4.1.3
expression in Escherichia coli
Halodesulfovibrio aestuarii
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.4.1.3
N,N,N',N'-tetrakis(2-pyridylmethyl)ethane-1,2-diamine
selectively inhibits all known members of the cupin-DMSP lyases and lyase-like-superfamily that exhibit DMSP lyase activity
Alcaligenes faecalis
4.4.1.3
N,N,N',N'-tetrakis(2-pyridylmethyl)ethane-1,2-diamine
selectively inhibits all known members of the cupin-DMSP lyases and lyase-like-superfamily that exhibit DMSP lyase activity
Halodesulfovibrio aestuarii
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.4.1.3
0.85
-
beta-dimethylsulfoniopropanoate
pH 8.0, 30C
Halodesulfovibrio aestuarii
4.4.1.3
2.56
-
beta-dimethylsulfoniopropanoate
pH 8.0, 30C
Alcaligenes faecalis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.4.1.3
Ca2+
-
Alcaligenes faecalis
4.4.1.3
Ca2+
-
Halodesulfovibrio aestuarii
4.4.1.3
Mn2+
-
Alcaligenes faecalis
4.4.1.3
Mn2+
-
Halodesulfovibrio aestuarii
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.4.1.3
Alcaligenes faecalis
E7DDH2
-
-
4.4.1.3
Halodesulfovibrio aestuarii
A0A1M6LQI1
-
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4.4.1.3
902
-
pH 8, 30C
Alcaligenes faecalis
4.4.1.3
1391
-
pH 8, 30C
Halodesulfovibrio aestuarii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.4.1.3
beta-dimethylsulfoniopropanoate
-
747144
Alcaligenes faecalis
dimethylsulfide + acrylate
-
-
-
?
4.4.1.3
beta-dimethylsulfoniopropanoate
-
747144
Halodesulfovibrio aestuarii
dimethylsulfide + acrylate
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.4.1.3
900
-
beta-dimethylsulfoniopropanoate
pH 8.0, 30C
Alcaligenes faecalis
4.4.1.3
1130
-
beta-dimethylsulfoniopropanoate
pH 8.0, 30C
Halodesulfovibrio aestuarii
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.4.1.3
expression in Escherichia coli
Alcaligenes faecalis
4.4.1.3
expression in Escherichia coli
Halodesulfovibrio aestuarii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.4.1.3
N,N,N',N'-tetrakis(2-pyridylmethyl)ethane-1,2-diamine
selectively inhibits all known members of the cupin-DMSP lyases and lyase-like-superfamily that exhibit DMSP lyase activity
Alcaligenes faecalis
4.4.1.3
N,N,N',N'-tetrakis(2-pyridylmethyl)ethane-1,2-diamine
selectively inhibits all known members of the cupin-DMSP lyases and lyase-like-superfamily that exhibit DMSP lyase activity
Halodesulfovibrio aestuarii
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.4.1.3
0.85
-
beta-dimethylsulfoniopropanoate
pH 8.0, 30C
Halodesulfovibrio aestuarii
4.4.1.3
2.56
-
beta-dimethylsulfoniopropanoate
pH 8.0, 30C
Alcaligenes faecalis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.4.1.3
Ca2+
-
Alcaligenes faecalis
4.4.1.3
Ca2+
-
Halodesulfovibrio aestuarii
4.4.1.3
Mn2+
-
Alcaligenes faecalis
4.4.1.3
Mn2+
-
Halodesulfovibrio aestuarii
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4.4.1.3
902
-
pH 8, 30C
Alcaligenes faecalis
4.4.1.3
1391
-
pH 8, 30C
Halodesulfovibrio aestuarii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.4.1.3
beta-dimethylsulfoniopropanoate
-
747144
Alcaligenes faecalis
dimethylsulfide + acrylate
-
-
-
?
4.4.1.3
beta-dimethylsulfoniopropanoate
-
747144
Halodesulfovibrio aestuarii
dimethylsulfide + acrylate
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.4.1.3
900
-
beta-dimethylsulfoniopropanoate
pH 8.0, 30C
Alcaligenes faecalis
4.4.1.3
1130
-
beta-dimethylsulfoniopropanoate
pH 8.0, 30C
Halodesulfovibrio aestuarii
General Information
EC Number
General Information
Commentary
Organism
4.4.1.3
physiological function
comparison of DMSP lyases and lyase-like enzymes. Two conserved sequence motifs of the superfamily include the metal-ligating residues that are absolutely essential and other residues including an active site tyrosine that seems to play a relatively minor role in DMSP lysis
Alcaligenes faecalis
4.4.1.3
physiological function
comparison of DMSP lyases and lyase-like enzymes. Two conserved sequence motifs of the superfamily include the metal-ligating residues that are absolutely essential and other residues including an active site tyrosine that seems to play a relatively minor role in DMSP lysis
Halodesulfovibrio aestuarii
General Information (protein specific)
EC Number
General Information
Commentary
Organism
4.4.1.3
physiological function
comparison of DMSP lyases and lyase-like enzymes. Two conserved sequence motifs of the superfamily include the metal-ligating residues that are absolutely essential and other residues including an active site tyrosine that seems to play a relatively minor role in DMSP lysis
Alcaligenes faecalis
4.4.1.3
physiological function
comparison of DMSP lyases and lyase-like enzymes. Two conserved sequence motifs of the superfamily include the metal-ligating residues that are absolutely essential and other residues including an active site tyrosine that seems to play a relatively minor role in DMSP lysis
Halodesulfovibrio aestuarii
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.4.1.3
350
-
beta-dimethylsulfoniopropanoate
pH 8.0, 30C
Alcaligenes faecalis
4.4.1.3
1320
-
beta-dimethylsulfoniopropanoate
pH 8.0, 30C
Halodesulfovibrio aestuarii
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.4.1.3
350
-
beta-dimethylsulfoniopropanoate
pH 8.0, 30C
Alcaligenes faecalis
4.4.1.3
1320
-
beta-dimethylsulfoniopropanoate
pH 8.0, 30C
Halodesulfovibrio aestuarii