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Literature summary extracted from

  • Lei, L.; Cherukuri, K.P.; Alcolombri, U.; Meltzer, D.; Tawfik, D.S.
    The dimethylsulfoniopropionate (DMSP) lyase and lyase-like cupin family consists of bona fide DMSP lyases as well as other enzymes with unknown function (2018), Biochemistry, 57, 3364-3377 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.3 expression in Escherichia coli Alcaligenes faecalis
4.4.1.3 expression in Escherichia coli Halodesulfovibrio aestuarii

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.3 N,N,N',N'-tetrakis(2-pyridylmethyl)ethane-1,2-diamine selectively inhibits all known members of the cupin-DMSP lyases and lyase-like-superfamily that exhibit DMSP lyase activity Alcaligenes faecalis
4.4.1.3 N,N,N',N'-tetrakis(2-pyridylmethyl)ethane-1,2-diamine selectively inhibits all known members of the cupin-DMSP lyases and lyase-like-superfamily that exhibit DMSP lyase activity Halodesulfovibrio aestuarii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.3 0.85
-
 beta-dimethylsulfoniopropanoate pH 8.0, 30°C Halodesulfovibrio aestuarii
4.4.1.3 2.56
-
 beta-dimethylsulfoniopropanoate pH 8.0, 30°C Alcaligenes faecalis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.4.1.3 Ca2+
-
 Alcaligenes faecalis
4.4.1.3 Ca2+
-
 Halodesulfovibrio aestuarii
4.4.1.3 Mn2+
-
 Alcaligenes faecalis
4.4.1.3 Mn2+
-
 Halodesulfovibrio aestuarii

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.3 Alcaligenes faecalis E7DDH2
-
-
4.4.1.3 Halodesulfovibrio aestuarii A0A8G2CC70
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.3 902
-
 pH 8, 30°C Alcaligenes faecalis
4.4.1.3 1391
-
 pH 8, 30°C Halodesulfovibrio aestuarii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.3 beta-dimethylsulfoniopropanoate
-
 Alcaligenes faecalis dimethylsulfide + acrylate
-
 ?
4.4.1.3 beta-dimethylsulfoniopropanoate
-
 Halodesulfovibrio aestuarii dimethylsulfide + acrylate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.4.1.3 Dddy
-
 Alcaligenes faecalis
4.4.1.3 Dddy
-
 Halodesulfovibrio aestuarii
4.4.1.3 SAMN05660830_03106
-
 Halodesulfovibrio aestuarii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4.1.3 900
-
 beta-dimethylsulfoniopropanoate pH 8.0, 30°C Alcaligenes faecalis
4.4.1.3 1130
-
 beta-dimethylsulfoniopropanoate pH 8.0, 30°C Halodesulfovibrio aestuarii

General Information

EC Number General Information Comment Organism
4.4.1.3 physiological function comparison of DMSP lyases and lyase-like enzymes. Two conserved sequence motifs of the superfamily include the metal-ligating residues that are absolutely essential and other residues including an active site tyrosine that seems to play a relatively minor role in DMSP lysis Alcaligenes faecalis
4.4.1.3 physiological function comparison of DMSP lyases and lyase-like enzymes. Two conserved sequence motifs of the superfamily include the metal-ligating residues that are absolutely essential and other residues including an active site tyrosine that seems to play a relatively minor role in DMSP lysis Halodesulfovibrio aestuarii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.4.1.3 350
-
 beta-dimethylsulfoniopropanoate pH 8.0, 30°C Alcaligenes faecalis
4.4.1.3 1320
-
 beta-dimethylsulfoniopropanoate pH 8.0, 30°C Halodesulfovibrio aestuarii