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Literature summary extracted from

  • Schlee, S.; Klein, T.; Schumacher, M.; Nazet, J.; Merkl, R.; Steinhoff, H.J.; Sterner, R.
    Relationship of Catalysis and Active Site Loop Dynamics in the (betaalpha)8 barrel enzyme indole-3-glycerol phosphate synthase (2018), Biochemistry, 57, 3265-3277 .
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.48 molecular dynamics simulations of wild-type at 25°C in the apo form and in the presence of the substrate Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.48 F246S mutation at C-terminus, increases flexibility Saccharolobus solfataricus
4.1.1.48 G212E mutation interferes with phosphate binding Saccharolobus solfataricus
4.1.1.48 L236Q mutation destabilizes helix alpha8' Saccharolobus solfataricus
4.1.1.48 M237T mutation destabilizes helix alpha8' Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.48 0.000085
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate wild-type, pH 7.5, 25°C Saccharolobus solfataricus
4.1.1.48 0.000105
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate wild-type, pH 7.5, 60°C Saccharolobus solfataricus
4.1.1.48 0.0013
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate mutant F246S, pH 7.5, 25°C Saccharolobus solfataricus
4.1.1.48 0.0029
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate mutant M237T, pH 7.5, 25°C Saccharolobus solfataricus
4.1.1.48 0.0034
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate mutant L236Q, pH 7.5, 25°C Saccharolobus solfataricus
4.1.1.48 0.0217
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate mutant G212E, pH 7.5, 25°C Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.48 Saccharolobus solfataricus Q06121
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.48 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 Saccharolobus solfataricus 1-(3-indolyl)glycerol-3-phosphate + CO2 + H2O
-
 ?
4.1.1.48 additional information a different degree of loop mobility correlates with different rate-limiting steps in the catalytic mechanism. Both the application of activating mutations and temperature increase lead to a net increase in the catalytic turnover number due to acceleration of the product release rate relative to the chemical transformation steps Saccharolobus solfataricus ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.48 0.11
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate wild-type, pH 7.5, 25°C Saccharolobus solfataricus
4.1.1.48 0.16
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate mutant G212E, pH 7.5, 25°C Saccharolobus solfataricus
4.1.1.48 0.2
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate mutant L236Q, pH 7.5, 25°C Saccharolobus solfataricus
4.1.1.48 0.23
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate mutant M237T, pH 7.5, 25°C Saccharolobus solfataricus
4.1.1.48 0.32
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate mutant F246S, pH 7.5, 25°C Saccharolobus solfataricus
4.1.1.48 2.1
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate wild-type, pH 7.5, 60°C Saccharolobus solfataricus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.1.48 10
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate mutant G212E, pH 7.5, 25°C Saccharolobus solfataricus
4.1.1.48 60
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate mutant L236Q, pH 7.5, 25°C Saccharolobus solfataricus
4.1.1.48 80
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate mutant M237T, pH 7.5, 25°C Saccharolobus solfataricus
4.1.1.48 240
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate mutant F246S, pH 7.5, 25°C Saccharolobus solfataricus
4.1.1.48 1180
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate wild-type, pH 7.5, 25°C Saccharolobus solfataricus
4.1.1.48 20000
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate wild-type, pH 7.5, 60°C Saccharolobus solfataricus