BRENDA - Enzyme Database

Iron binding properties of recombinant class a protein disulfide isomerase from Arabidopsis thaliana

Remelli, W.; Santabarbara, S.; Carbonera, D.; Bonomi, F.; Ceriotti, A.; Casazza, A.P.; Biochemistry 56, 2116-2125 (2017)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
5.3.4.1
C51S
the mutant shows reduced reductase and oxidase activities compared to the recombinant wild type enzyme PDI-A
Arabidopsis thaliana
5.3.4.1
C55S
the mutant shows reduced reductase and oxidase activities compared to the recombinant wild type enzyme PDI-A
Arabidopsis thaliana
5.3.4.1
C85S/C92S
the mutant shows reduced reductase and oxidase activities compared to the recombinant wild type enzyme PDI-A
Arabidopsis thaliana
5.3.4.1
C92S
the mutant shows reduced reductase and oxidase activities compared to the recombinant wild type enzyme PDI-A
Arabidopsis thaliana
5.3.4.1
K56G
the mutant shows reduced reductase and oxidase activities compared to the recombinant wild type enzyme PDI-A
Arabidopsis thaliana
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.4.1
endoplasmic reticulum
-
Arabidopsis thaliana
5783
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.4.1
Arabidopsis thaliana
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.3.4.1
Ni-NTA resin column chromatography and DEAE column chromatography
Arabidopsis thaliana
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.4.1
NRCSQGSCWN
-
747122
Arabidopsis thaliana
?
-
-
-
?
5.3.4.1
oxidized insulin
-
747122
Arabidopsis thaliana
reduced insulin
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
5.3.4.1
?
x * 14000, SDS-PAGE
Arabidopsis thaliana
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.3.4.1
[2Fe-2S]-center
-
Arabidopsis thaliana
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.3.4.1
[2Fe-2S]-center
-
Arabidopsis thaliana
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
5.3.4.1
C51S
the mutant shows reduced reductase and oxidase activities compared to the recombinant wild type enzyme PDI-A
Arabidopsis thaliana
5.3.4.1
C55S
the mutant shows reduced reductase and oxidase activities compared to the recombinant wild type enzyme PDI-A
Arabidopsis thaliana
5.3.4.1
C85S/C92S
the mutant shows reduced reductase and oxidase activities compared to the recombinant wild type enzyme PDI-A
Arabidopsis thaliana
5.3.4.1
C92S
the mutant shows reduced reductase and oxidase activities compared to the recombinant wild type enzyme PDI-A
Arabidopsis thaliana
5.3.4.1
K56G
the mutant shows reduced reductase and oxidase activities compared to the recombinant wild type enzyme PDI-A
Arabidopsis thaliana
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.4.1
endoplasmic reticulum
-
Arabidopsis thaliana
5783
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.4.1
Ni-NTA resin column chromatography and DEAE column chromatography
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.4.1
NRCSQGSCWN
-
747122
Arabidopsis thaliana
?
-
-
-
?
5.3.4.1
oxidized insulin
-
747122
Arabidopsis thaliana
reduced insulin
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.3.4.1
?
x * 14000, SDS-PAGE
Arabidopsis thaliana