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Literature summary extracted from

  • Morehouse, B.R.; Kumar, R.P.; Matos, J.O.; Olsen, S.N.; Entova, S.; Oprian, D.D.
    Functional and structural characterization of a (+)-limonene synthase from Citrus sinensis (2017), Biochemistry, 56, 1706-1715 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.20 expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL cells Citrus sinensis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.3.20 apoenzyme, sitting drop vapor diffusion method, using 12-16% (w/v) PEG 8000 as precipitant Citrus sinensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.20 Mn2+ the enzyme is strongly inhibited at concentrations of more than 1 mM Citrus sinensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.20 0.0131
-
geranyl diphosphate at pH 8.0, temperature not specified in the publication Citrus sinensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.20 Mg2+ may replace Mn2+ but with much lower efficiency (maximum activity at 20 mM) Citrus sinensis
4.2.3.20 Mn2+ required for activity, 0.4 mM used in assay conditions Citrus sinensis
4.2.3.20 additional information not affected by K+ and Na+ Citrus sinensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.20 70000
-
gel filtration Citrus sinensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.20 geranyl diphosphate Citrus sinensis
-
(R)-limonene + diphosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.20 Citrus sinensis A0A1C9J6A7 navel orange
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.20 Ni-Sepharose column chromatography Citrus sinensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.20 geranyl diphosphate
-
Citrus sinensis (R)-limonene + diphosphate
-
?

Subunits

EC Number Subunits Comment Organism
4.2.3.20 monomer 1 * 70000, SDS-PAGE Citrus sinensis
4.2.3.20 monomer 1 * 65700, calculated from amino acid sequence Citrus sinensis

Synonyms

EC Number Synonyms Comment Organism
4.2.3.20 (+)-limonene synthase
-
Citrus sinensis
4.2.3.20 (+)-LS
-
Citrus sinensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.20 0.186
-
geranyl diphosphate at pH 8.0, temperature not specified in the publication Citrus sinensis