Literature summary extracted from
Morehouse, B.R.; Kumar, R.P.; Matos, J.O.; Olsen, S.N.; Entova, S.; Oprian, D.D.
Functional and structural characterization of a (+)-limonene synthase from Citrus sinensis (2017), Biochemistry, 56, 1706-1715 .
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
4.2.3.20 |
expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL cells |
Citrus sinensis |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
4.2.3.20 |
apoenzyme, sitting drop vapor diffusion method, using 12-16% (w/v) PEG 8000 as precipitant |
Citrus sinensis |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
4.2.3.20 |
Mn2+ |
the enzyme is strongly inhibited at concentrations of more than 1 mM |
Citrus sinensis |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
4.2.3.20 |
0.0131 |
- |
geranyl diphosphate |
at pH 8.0, temperature not specified in the publication |
Citrus sinensis |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
4.2.3.20 |
Mg2+ |
may replace Mn2+ but with much lower efficiency (maximum activity at 20 mM) |
Citrus sinensis |
|
4.2.3.20 |
Mn2+ |
required for activity, 0.4 mM used in assay conditions |
Citrus sinensis |
|
4.2.3.20 |
additional information |
not affected by K+ and Na+ |
Citrus sinensis |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
4.2.3.20 |
70000 |
- |
gel filtration |
Citrus sinensis |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
4.2.3.20 |
geranyl diphosphate |
Citrus sinensis |
- |
(R)-limonene + diphosphate |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.2.3.20 |
Citrus sinensis |
A0A1C9J6A7 |
navel orange |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
4.2.3.20 |
Ni-Sepharose column chromatography |
Citrus sinensis |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.2.3.20 |
geranyl diphosphate |
- |
Citrus sinensis |
(R)-limonene + diphosphate |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
4.2.3.20 |
monomer |
1 * 70000, SDS-PAGE |
Citrus sinensis |
4.2.3.20 |
monomer |
1 * 65700, calculated from amino acid sequence |
Citrus sinensis |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
4.2.3.20 |
(+)-limonene synthase |
- |
Citrus sinensis |
4.2.3.20 |
(+)-LS |
- |
Citrus sinensis |
Turnover Number [1/s]
EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Comment |
Organism |
Structure |
---|
4.2.3.20 |
0.186 |
- |
geranyl diphosphate |
at pH 8.0, temperature not specified in the publication |
Citrus sinensis |
|